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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Normlizer420]
    #26925072 -

Yes. Good to see members from almost 20 years ago still around!


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #26925175 -

I’ve had a couple swabs that I streaked a bunch of plates and got nothing until I cut the whole handle off and jammed the swab into the agar.

Some people do the smarter version and tweezer some threads off to stick in the plate but I couldn’t get any to come loose.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario]
    #26925448 -

Thanks for the reply, I think I'll just have to transfer out what I come across and see what happens, learning experience.


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Ignored by me? Leave a rating.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Darkshadow]
    #26926520 -

.

Edited by PNDub (09/24/20 05:21 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PNDub]
    #26927875 -

PNDub said:
This is my first attempt with agar, looking for some opinions, please!  Do you see anything worth transferring??  Any and all feedback welcome.

These are from a print I took from a huge Amazonian cap I harvested:


Anyone see anything they would transfer?  Are the pics clear enough?  I've done TONS of reading on this, would love some feedback if anyone has a spare minute!!  Thank you :smile:


Hey man, I don't know which ones are best to harvest, but if you find out, can you tell me? I have some at the same stage by the look of it 10 days.

My plan was to just start picking out bits that start looking ropey and grain spawn from that but not sure now. what do you know?

Cheers


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====================================


We're all going to die

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Re: AGAR ENVY! (Anything and All things agar!) [Re: redpillcottage]
    #26927898 -

Hey red, :grin: it is perfectly acceptable for you to post pics as well to ask people about yours too.., no need to wait for people to give advice to some random dude for his trays, as his trays are not your trays lol :lol:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26928040 -

So this is an all out attempt to reduce the rate of auto-contams running about 30-40% currently.
Just say no to immaculate contamination!

9/9 Wednesday - Plates, Corning Borosilicate 100x15mm
20 plates in foil oven sterilized, 400 F, 2 hrs. ... should be 450 F
Cool down in oven 2 hrs
Carry in shoeboxes to SAB

9/10 9:00 Thursday - “Team Telephone” Agar
8g 1.6% Agar, 7.5g 1.5% Pilsner LME, green, no broth
Soak LME in 500mL bottled water before - 1 hr
Agar 20 min PC, took at least 20 to cool till pressure lock clicked.
Then opened the PC and tightened the media cap with foil still on.
Then off to the SAB to cool to 120F, this time.
Washed 90 Qrt SAB, rack, and table, w/Clorox & towel
Books on top of SAB to anchor it down and reduce effects of bumping.
Wet towel under 3” high rack. Foil wrapped plates in SAB.
Pour in 2 hrs.
What a pain taking the foil off in the SAB
Media bottle ring fell off, but only a couple drips.
I marked the drip spots on each plate with a Sharpie.
Maybe 200mL left over, some extra plates on top, to reduce condensation, of 4 stacks of 5 ea. 11:00
Oooh, really clear green!
Not going to let them sit too long before wrapping stacks of 5.
Trying to avoid auto-contam, immaculate contam.
But if there’s too much condensation,
then I’ll try putting a plate sleeve on them and cool next time...
Also this time, I have a sheet over the SAB,
because I might have a visitor.
12:00 wrapped! That’s a wrap!    :awedance:


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:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: redpillcottage]
    #26928154 -

.

Edited by PNDub (09/24/20 05:21 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PNDub]
    #26928541 -

Hey. So I’m on my second transfer of PE. I cut a large wedge of some rhyzomorphic myc which has continued to grow but it’s hovering over the new agar. My question is would you guys cut the wedge into thirds while the myc is strong and place onto new plates or just let it keep growing out until it hits the plate on its own. I know it was a large transfer but I’m still not great with the scalpel and I’m paranoid about contams so I was working quickly. I’m working on purging my agar thinner so this doesn’t happen again.

Another question. Do you guys transfer as soon as you think you have contamination on the plate or do you wait until the myc grows out but is still away from contamination. The plate has a spot of bacteria but it’s small so far. Thanks!




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LAGM 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Keefersutherland] * 1
    #26928545 -

I would transfer in 2 or 3 days personally.

Mold i try to transfer away from asap. But bacterial sattelites usually stay put


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Keefersutherland]
    #26928550 -

Depends what the contam is.

Mycelium generally can "outrun" bacteria efficienctly enough that waiting for it to grow out more will work well.

If it's thought to be contaminated with some type of sporulating mold...well then transfering ASAP is ideal.



So there are different answers I guess depending on what you're dealing with. Hopefully that provides some insight. Surely some other members have advice on this too.


I'm working on a RW clone culture but the mycelium is looking iffy. I think it might be the formula I've been using for agar as of late but IDK. Might try to whip up a batch with some grain soak water, see if I can get better looking growth :confused:

It's a seriously kick ass clone so, I reallly don't want to give up. Worried it has some hidden mold or something but who knows. The investigation continues



edit:

I really need to get on my slant game. Kind of embarassing to admit I've never made a slant in my life


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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #26928551 -

tryptkaloids said:
I would transfer in 2 or 3 days personally.

Mold i try to transfer away from asap. But bacterial sattelites usually stay put



Basically what I said in 1 sentence instead of several  :lol:

trypt :thumbup:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: sh4d0ws]
    #26928561 -

I need to make slants too.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #26928605 -

How does this plate look?  I've never had one try to pin so hard in the dish.  Worried something might be off.





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Re: AGAR ENVY! (Anything and All things agar!) [Re: mcnugget]
    #26928646 -

Thanks. I’ll wait a few days and post the results.


--------------------



LAGM 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ruK]
    #26928653 -

ruK said:


Lil B+ snowflake on PDA.


That's some clear pda you made. How did you filter it?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #26928981 -

hey y'all
first time posting
first time pouring agar (telephone+LME)

first run was a little bumpy... er... chunky... but poured some decent plates and learned a lot along the way, pumped to get something growing on these ^_^

s/o to everyone on here for the knowledge this place is incredible <3


Edited by Alvina (09/10/20 10:45 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Alvina]
    #26928998 -

Alvina said:
hey y'all
first time posting
first time pouring agar (telephone+LME)

first run was a little bumpy... er... chunky... but poured some decent plates and learned a lot along the way, pumped to get something growing on these ^_^

s/o to everyone on here for the knowledge this place is incredible <3




What are you gonna put on those? I'm at the tail end of a spore print giveaway if you need something to put on those plates! Shoot me a pm if you're interested!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #26929030 -

First PF classic transfer to MEA

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #26929037 -

roarkell said:
Alvina said:
hey y'all
first time posting
first time pouring agar (telephone+LME)

first run was a little bumpy... er... chunky... but poured some decent plates and learned a lot along the way, pumped to get something growing on these ^_^

s/o to everyone on here for the knowledge this place is incredible <3




What are you gonna put on those? I'm at the tail end of a spore print giveaway if you need something to put on those plates! Shoot me a pm if you're interested!


I've got some golden teacher LC en route and a probably filthy cambodian print from my first grow earlier this year :crazy:

I'll slide you a PM :grin:

Edited by Alvina (09/10/20 11:08 PM)

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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