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Doctor Mario
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: ruK]
#26914245 - 09/02/20 06:14 PM (5 years, 11 months ago)

ruK said:

A couple of days, but it depends on the culture. Requires some vigilance. It's mainly for bacteria-ridden plates, in my experience. Some swab plates that had competitor molds on them ended up beating the mycelium to the surface, in which case I had to start over. Myc seems to reliably beat bacteria, though.

Okay. I suppose I'm just being impatient. Its only been 48 hours since I swabbed plates and Its bothering me that there's not even bacteria showing lol. I'll forget about em for a few days.

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Rafiikii

Registered: 11/17/10
Posts: 2,892

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#26914253 - 09/02/20 06:16 PM (5 years, 11 months ago)

has any one used or considered reusing old cracker dry Petri dishes?

I have a handful of plates lying around from over the past however many months, years etc that look like this.

Still micropore taped, and also previously clean culture.

open under the flow hood remove the dried up agar, and pour again

thoughts?

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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."

Edited by Rafiikii (09/02/20 06:17 PM)

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tryptkaloids
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#26914256 - 09/02/20 06:17 PM (5 years, 11 months ago)

Germination can take up to 2 weeks. Imo a lot of recipes aren't great for germination, i like to dail back on the agar a tad and up on the nutes for germ plates

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
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tryptkaloids
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#26914284 - 09/02/20 06:22 PM (5 years, 11 months ago)

Thats exactly what i did to make this plate, a tranfer later it looks great! It wasnt that old, but it should still work.

Thhe donor plate was poured so thin i didn't even get any agar, i just scraped up some myc and transferred it

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

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MLPismyOPSEC
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#26914292 - 09/02/20 06:23 PM (5 years, 11 months ago)

tryptkaloids said:

Bacteria can't handle as much heat as myc so when it gets meshed into a culture it helps to pour warm agar over the culture and letting it grow through it before you take your transfer.

I think i know the answer to this but i assume you would want to do a hot pour while the culture is the size of a quarter or half dollar, then wait til the second layer of agar gets more fully colonized before transferring? Haven't done a hot pour yet, but hella considering it, i either have a couple cultures with bacteria intertwined or my grain prep is shit [most likely shit grain prep tbh].

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Doctor Mario
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#26914314 - 09/02/20 06:26 PM (5 years, 11 months ago)

tryptkaloids said:

Germination can take up to 2 weeks. Imo a lot of recipes aren't great for germination, i like to dail back on the agar a tad and up on the nutes for germ plates

Oh damn I thought I read somewhere that less nutes will make the plate colonize faster.

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Rafiikii

Registered: 11/17/10
Posts: 2,892

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#26914339 - 09/02/20 06:37 PM (5 years, 11 months ago)

tryptkaloids said:

Thats exactly what i did to make this plate, a tranfer later it looks great! It wasnt that old, but it should still work.

Thhe donor plate was poured so thin i didn't even get any agar, i just scraped up some myc and transferred it

I simply mean reusing the plate itself, the agar drops out like a cracker lol

I poured some reused plates earlier we shall see how it goes.

plates are cheap but ive kept all of them from over the years. figured It would be worth a try instead of just tossing them.

never seen this discussed before

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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."

Edited by Rafiikii (09/02/20 06:44 PM)

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Hobbit GDF
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Rafiikii]
#26914448 - 09/02/20 07:41 PM (5 years, 11 months ago)

Here are a few that are going to grain soon.. I've been alternating between transfers with dog food agar, malt extract agar, potato flake agar, & brown rice flour agar.

Yeti
Melmac F2
White tailfeathers F2
Ape (kingkong)
Tailfeathers clone4 (one plate has mold ,one has bacteria but usable)

Not included in the pictures....
Burma swab plate & T1
Golden teacher "clusterfuck" swab plate
The GT"cf" is one of the tailfeathers parents.

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maniac1886
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Rafiikii]
#26914449 - 09/02/20 07:43 PM (5 years, 11 months ago)

So my first attempt at agar work was a huge failure. I originally bought the wrong kind of agar. The kind that is for bacterial growth. I tried adding potato flakes to it and was pouring it in glass petri dishes. Im pretty sure I had a dirty syring to boot. None the less, it was complete failure over and over again.

Ive since made some pasty plates, and purchased some MEA premixed agar. I also got a new MS syringe from a vendor.

I didnt use a loop, kinda forgot about those. I just shot a drop out of the syringe on the agar in an SAB. The needle was still hot so it hissed and popped, looks like the only spores that didnt die of heat made it to the outside wall. Just made my first transfer 9 days after inoculation. Its hard to see the culture in these pasty plates, but it looks like im finally making headway. I did a transfer today, and I also dropped a drop of LC I made with WBS soak water and the same MS.

Here is a couple pics (although poor) of my plate. One with flash, one without.

Edited by maniac1886 (09/02/20 07:45 PM)

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A.k.a
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: maniac1886]
#26914459 - 09/02/20 07:50 PM (5 years, 11 months ago)

Low nutes does make it grow a little faster but that’s for myc transfers.

For spores high nute with soft agar is best.

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tryptkaloids
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#26914527 - 09/02/20 08:27 PM (5 years, 11 months ago)

I wouldnt even think you need to take out the old puck. Just pour over it it the culture was clean

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

Doctor Mario
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Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#26914552 - 09/02/20 08:50 PM (5 years, 11 months ago)

A.k.a said:

Low nutes does make it grow a little faster but that’s for myc transfers.

For spores high nute with soft agar is best.

Hmm... I did 10g Agar Agar and 8g Light Malt Extract per 500mL distilled water. Where does that fall into the high/low Nute/Agar category?

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tryptkaloids
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#26914588 - 09/02/20 09:16 PM (5 years, 11 months ago)

Sounds great for transfers, a bit stiff for germination. Its all about how much access the spores have to the nutrients and water. They cant get to it if it's locked away too mucj

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

Doctor Mario
🍄🍄🍄🍄🍄

Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#26914596 - 09/02/20 09:22 PM (5 years, 11 months ago)

tryptkaloids said:

Sounds great for transfers, a bit stiff for germination. Its all about how much access the spores have to the nutrients and water. They cant get to it if it's locked away too mucj

Damn... I hope it works out. I nocced up 10 more plates with that recipe tonight. Using those ingredients, what ratio do you recommend for germination?

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Hobbit GDF
Deadhead

Registered: 02/14/19
Posts: 4,441
Loc: Terrapin station

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#26914712 - 09/02/20 10:36 PM (5 years, 11 months ago)

Listen I do 10g agar , 7.5 nutes , 500ml h20 and I get good germination rates from spores. You'll be fine

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ruK
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Registered: 05/27/20
Posts: 83
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#26914726 - 09/02/20 10:44 PM (5 years, 11 months ago)

Doctor Mario said:

ruK said:

A couple of days, but it depends on the culture. Requires some vigilance. It's mainly for bacteria-ridden plates, in my experience. Some swab plates that had competitor molds on them ended up beating the mycelium to the surface, in which case I had to start over. Myc seems to reliably beat bacteria, though.

Okay. I suppose I'm just being impatient. Its only been 48 hours since I swabbed plates and Its bothering me that there's not even bacteria showing lol. I'll forget about em for a few days.

You got a ways to go on germ plates. They can take forever.

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Rafiikii

Registered: 11/17/10
Posts: 2,892

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#26914744 - 09/02/20 11:01 PM (5 years, 11 months ago)

tryptkaloids said:

I wouldnt even think you need to take out the old puck. Just pour over it it the culture was clean

so easy to take out though,..ill report back, taking some clones tomorrow.

--------------------
"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."

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A.k.a
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Rafiikii]
#26914895 - 09/03/20 03:24 AM (5 years, 11 months ago)

That’s a fairly average recipe. Like he said you’ll be ok, I’ve never had problems with plates like that either. The only time I had spores take forever was on plates with like 3g lme for 500ml.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#26914951 - 09/03/20 05:13 AM (5 years, 11 months ago)

I still can’t get ape to germ. Gone through 5 or 6 swabs already

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#26914954 - 09/03/20 05:14 AM (5 years, 11 months ago)

Even burying the swabs?

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