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Doctor Mario
🍄🍄🍄🍄🍄

Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸

Re: AGAR ENVY! (Anything and All things agar!)

[Re: neef]
#26911806 - 09/01/20 12:12 PM (5 years, 11 months ago)

I poured and nocced plates for the first time last night. The plates that I nocced were still pretty liquefied and the nocc loop went inside of the agar instead of across the top. Assuming that the spores will germinate is this okay or will the mycelium not be able to breathe?

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Puduwoke
mushroom enthusiastic

Registered: 06/25/17
Posts: 2,005
Last seen: 13 days, 21 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#26911952 - 09/01/20 01:11 PM (5 years, 11 months ago)

Wont be a problem

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Doctor Mario
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Registered: 08/07/20
Posts: 1,894
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Puduwoke]
#26912000 - 09/01/20 01:40 PM (5 years, 11 months ago)

Puduwoke said:

Wont be a problem

👍 Thank you.

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ruK
Stranger

Registered: 05/27/20
Posts: 83
Last seen: 4 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#26912753 - 09/01/20 08:37 PM (5 years, 11 months ago)

Lil B+ snowflake on PDA.

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ruK
Stranger

Registered: 05/27/20
Posts: 83
Last seen: 4 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: dirtybirdx]
#26912757 - 09/01/20 08:40 PM (5 years, 11 months ago)

dirtybirdx said:

Wanted to show you guys/gals a nifty storage drawer that I’ve been using for room temp agar cultures. You could fit 216 60mm petris, or 96 90mm petris, and allows you to easily categorize and sort through them.

Also did a big block of SAB work last night - 30 transfers Costa Rican —>t4 / 6 transfers Malabar —>t2 / 4 transfers Costa Rican —>t5

Took some pics of the prettier ones:

Ahh that's dope! I was considering getting the same type of storage cabinet for the worktable. Only thing that stopped me was how inefficient it'd be for the 100x15 plates vs the smaller ones.

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MH5109
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Registered: 09/27/17
Posts: 1,385
Loc: America

Last seen: 5 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ruK]
#26913094 - 09/02/20 02:43 AM (5 years, 11 months ago)

I have an aa culture that I I thought was toast, there was mold all the way around. Now I am confident there is nice looking mycelium in there that I want a piece of, going to try a hot pour tomorrow, see if I can salvage a viable bit.

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MadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ruK]
#26913104 - 09/02/20 03:18 AM (5 years, 11 months ago)

ruK said:

Lil B+ snowflake on PDA.

Sick plate :rockon:

:rockon:

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ruK
Stranger

Registered: 05/27/20
Posts: 83
Last seen: 4 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: MadHatter333]
#26913705 - 09/02/20 12:34 PM (5 years, 11 months ago)

MadHatter333 said:

ruK said:

Lil B+ snowflake on PDA.

Sick plate

Thanks <3. Finally getting nice even growth after spinning my wheels on MEA. For some reason this culture goes tomentose on half strength malt but throws ropes on full strength potato dextrose. :uhoh:

:uhoh:

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ruK
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: ruK]
#26913712 - 09/02/20 12:39 PM (5 years, 11 months ago)

This one's declaring war against its overlord oppressor. I'm kinda scared.

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Doctor Mario
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Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ruK]
#26913720 - 09/02/20 12:48 PM (5 years, 11 months ago)

Those are awesome. Whats causing them to grow like that?

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,339
Loc: Exact Center
Last seen: 10 days, 9 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#26913744 - 09/02/20 01:05 PM (5 years, 11 months ago)

The amount of genetics present

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

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ruK
Stranger

Registered: 05/27/20
Posts: 83
Last seen: 4 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#26913924 - 09/02/20 03:05 PM (5 years, 11 months ago)

Doctor Mario said:

Those are awesome. Whats causing them to grow like that?

tryptkaloids said:

The amount of genetics present

Yep, it's 3 transfers removed from a swab. Just cleaning it up. The original swab was a bacterial mess. Had to warm pour to get it clean.

Cute puppers btw trypt.

Edited by ruK (09/02/20 03:08 PM)

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tryptkaloids
Learner

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Posts: 16,339
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: ruK]
#26913939 - 09/02/20 03:15 PM (5 years, 11 months ago)

Thanks, i miss that little fucker. RIP :heart:

:heart:

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

Doctor Mario
🍄🍄🍄🍄🍄

Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ruK]
#26914013 - 09/02/20 03:56 PM (5 years, 11 months ago)

ruK said:

Doctor Mario said:

Those are awesome. Whats causing them to grow like that?

tryptkaloids said:

The amount of genetics present

Whats a warm/hot pour?

Yep, it's 3 transfers removed from a swab. Just cleaning it up. The original swab was a bacterial mess. Had to warm pour to get it clean.

Cute puppers btw trypt.

--------------------

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,339
Loc: Exact Center
Last seen: 10 days, 9 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#26914027 - 09/02/20 04:07 PM (5 years, 11 months ago)

Bacteria can't handle as much heat as myc so when it gets meshed into a culture it helps to pour warm agar over the culture and letting it grow through it before you take your transfer.

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

ruK
Stranger

Registered: 05/27/20
Posts: 83
Last seen: 4 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#26914074 - 09/02/20 04:39 PM (5 years, 11 months ago)

tryptkaloids said:

Bacteria can't handle as much heat as myc so when it gets meshed into a culture it helps to pour warm agar over the culture and letting it grow through it before you take your transfer.

Just don't do what I did with my first time trying warm pours which was not paying attention to the thickness of the original agar plate. Mindlessly pouring hot agar onto plates and boom, fucking overflow everywhere. SMH

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ruK
Stranger

Registered: 05/27/20
Posts: 83
Last seen: 4 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#26914075 - 09/02/20 04:40 PM (5 years, 11 months ago)

tryptkaloids said:

Thanks, i miss that little fucker. RIP

So tough when they go. :frown:

:frown:

Big ups to the small guy.

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Doctor Mario
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Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ruK]
#26914106 - 09/02/20 04:57 PM (5 years, 11 months ago)

Thats good to know. This is the first I've read about it. How long does it normally take for myc or bacteria and mold to start showing on plates?

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,339
Loc: Exact Center
Last seen: 10 days, 9 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ruK]
#26914146 - 09/02/20 05:17 PM (5 years, 11 months ago)

ruK said:

tryptkaloids said:

Bacteria can't handle as much heat as myc so when it gets meshed into a culture it helps to pour warm agar over the culture and letting it grow through it before you take your transfer.

Just don't do what I did with my first time trying warm pours which was not paying attention to the thickness of the original agar plate. Mindlessly pouring hot agar onto plates and boom, fucking overflow everywhere. SMH

:rofl:

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

ruK
Stranger

Registered: 05/27/20
Posts: 83
Last seen: 4 years, 11 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#26914200 - 09/02/20 05:58 PM (5 years, 11 months ago)

Doctor Mario said:

Thats good to know. This is the first I've read about it. How long does it normally take for myc or bacteria and mold to start showing on plates?

A couple of days, but it depends on the culture. Requires some vigilance. It's mainly for bacteria-ridden plates, in my experience. Some swab plates that had competitor molds on them ended up beating the mycelium to the surface, in which case I had to start over. Myc seems to reliably beat bacteria, though.

Edited by ruK (09/02/20 05:58 PM)

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Shop:

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Spores for European Microscopy

Golden Teacher Liquid Culture For Sale

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