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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smoothcat]
    #26511383 -

Smoothcat said:
I’ve made t-gel agar before which seemingly limits bacteria but I’d be interested in seeing how coffee agar performs


Well I upped my agar, cause for what ever reason I thought that it would be a good idea. So I did 14g agar to 250ml coffee and 250 water. Plates turned out harder than I like with LME. I just nocked up half of them with a bunch of old prints. Found Eats Burma circuit 2014. But I also transfered active cultures as a control.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Sockadin] * 1
    #26511385 -

That's a shit ton of agar

3x what I use for stiff agar, 6x what I use for soft agar


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Edited by c10h12n2o (03/01/20 03:10 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26511631 -

Yeah I shouldn't drink and cultivate at the same time. Hard to find time to cultivate if that was the case.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26511638 -

c10h12n2o said:
That's a shit ton of agar

3x what I use for stiff agar, 6x what I use for soft agar


I can't wait to get a print for RW.
I'm curious c10, what agar recipie would put RW spores to?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #26511649 -

For some reason rw is the only kind that gives me trouble. I swiped one print over and over for 2 months and nothing. Got a second one and swiped it 6 days ago and still nothing.

One of these days rusty. One of these days.


--------------------
LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #26511650 -

Inthepit said:
c10h12n2o said:
That's a shit ton of agar

3x what I use for stiff agar, 6x what I use for soft agar


I can't wait to get a print for RW.
I'm curious c10, what agar recipie would put RW spores to?


When my RW finish I will send you a print. PM me your addy. Also you can do 7g LME 7g agar. Works for me.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Sockadin] * 1
    #26511797 -

Inthepit said:
c10h12n2o said:
That's a shit ton of agar

3x what I use for stiff agar, 6x what I use for soft agar


I can't wait to get a print for RW.
I'm curious c10, what agar recipie would put RW spores to?


Same as any cube, I'd start with my standard half strength mea (20g agar, 15g LME, 1L water). If that doesnt work I'd try soft agar (11g agar, 15g LME , 1L water)

If that doesnt work id try hydrating the spores, hot pour, various ph, and activated charcoal, in that order

I've been having really bad luck germinating some of the older prints from my old collection lately

Sockadin said:
When my RW finish I will send you a print. PM me your addy. Also you can do 7g LME 7g agar. Works for me.


I'd also love a print if you could hook me up. I dont have RW yet


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26511843 -

Im a noob...if this is in the wrong place please let me know. SO These are the first plates I have ever poured or inoculated. Used a spore syringe. 1-2 drops. Is everything looking ok? Too early to tell..? How large should I let it get before I start any transfers?




Edited by MycPsilo (03/01/20 08:54 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26511844 -

I REALLY wanted to get into the RW’s and some kind member sent a print and they germinated within like 5days, I’m yet to grow them out but I’m super excited to get them going. When it gets to that stage I’ll defo be making prints and the mush love will be spread  :awesanta:



:loveheart:


--------------------
Back once again with the ill behaviour



Links I like

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Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26512049 -

c10h12n2o said:
Inthepit said:
c10h12n2o said:
That's a shit ton of agar

3x what I use for stiff agar, 6x what I use for soft agar


I can't wait to get a print for RW.
I'm curious c10, what agar recipie would put RW spores to?


Same as any cube, I'd start with my standard half strength mea (20g agar, 15g LME, 1L water). If that doesnt work I'd try soft agar (11g agar, 15g LME , 1L water)

If that doesnt work id try hydrating the spores, hot pour, various ph, and activated charcoal, in that order

I've been having really bad luck germinating some of the older prints from my old collection lately

Sockadin said:
When my RW finish I will send you a print. PM me your addy. Also you can do 7g LME 7g agar. Works for me.


I'd also love a print if you could hook me up. I dont have RW yet


If youre still in need of a RW you can message me a addy and I'll get you one in the mail bud. I'd be happy to send you one I have a active giveaway in the marketplace and I'm going to hit the post office to send some off tomorrow let me know.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: gizmo1]
    #26512064 -

Does anyone know the pros and cons of soft vs. hard agar?

I've been wondering if there was a time and place for one or the other.

I have: brf, potato flakes, karo, and some agar and was wondering if you guys have recipes you like with those things. About to streak some spores tomorrow.


--------------------
Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.

Edited by ichugwindex (03/02/20 12:51 AM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: ichugwindex]
    #26512128 -

Inthepit I'll gladly send you my extra rwbprint if you need one

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: ichugwindex]
    #26512213 -

ichugwindex said:
Does anyone know the pros and cons of soft vs. hard agar?

I've been wondering if there was a time and place for one or the other.

I have: brf, potato flakes, karo, and some agar and was wondering if you guys have recipes you like with those things. About to streak some spores tomorrow.


Those work but I'd say get some malt extract and keep things standard

Soft agar is harder to streak but better for spore germination (for spores that wont germinate on normal agar)


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26512221 -

I do want to try malt extract but haven't been able to find it locally


--------------------
Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: ichugwindex]
    #26512222 -

Just buy it on amazon for like 7$ for a lifetime supply


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26512230 -

c10h12n2o said:
Just buy it on amazon for like 7$ for a lifetime supply


Ok!


--------------------
Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: ichugwindex]
    #26512235 -

It's really useful to standardize your agar recipe because then you can actually make meaningful  comparisons between various plates


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Drboomer]
    #26512288 -

Drboomer said:
Inthepit I'll gladly send you my extra rwbprint if you need one


Oh thankyou that's really cool!
However I'm getting one already!
:nicetry:

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26512299 -

c10h12n2o said:
It's really useful to standardize your agar recipe because then you can actually make meaningful  comparisons between various plates


Do you ever change your agar recipe in between transfers to get the mycelium to change behaviors? I finally got RW fuzzy nightmare to calm down and get more organized decreasing my LME a couple of grams.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o]
    #26512497 -

I'm going to be using the rest of my no pour containers, so I'm going to be making a new batch of agar. I just want to make one uniform batch across for all of these. I'm going to be using them for swiping prints and transfers, everything, etc. Last time I used 9 grams agar/9 grams LME. I've also been recommended 7 grams agar/10 LME, that it will give me faster growth. What would you guys do or how to mix agar ratios that I can use for the rest of these no pours and that I will be using for everything, swipes, streaking, transfers, inoculating, etc. I just want to make a uniform batch with the rest of these no pour containers that will work the best for everything.


--------------------

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Shop: MagicBag.co Certified Organic All-In-One Grow Bags   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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