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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #26541061 -

Inthepit said:
tedoro said:
How do these look? Tell me everything. First transfer from different germ plates from different spore prints. All Golden Teacher. Thinly poured 20g agar 10g LME 1 liter. Thank you in advance.

Four plates, top and bottom. (1,2,3,4)






I gave up on my GT because they all seemed fluffy contam looking, all the way to T3s. So maybe I should have just knocked up a jar to see. :shrug:
You can see GT in my log, always fluffy, never rizo...


This is a good plate on agar with high nutes


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Re: AGAR ENVY! (Anything and All things agar!) [Re: tedoro]
    #26541063 -

tedoro said:
Is it okay to go to grain even if its just the first transfer? For the ones that look good?


Yes.

I didn't cause of the dogma.  Took T2 to grain.  Should have just gone with the T1.  But I'm still learning.

Then I have a different variety that is taking until T4 to be ready (except for one T3).

Once you know what to look for, go by the plate.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tedoro]
    #26541068 -

tedoro said:
tedoro said:
How do these look? Tell me everything. First transfer from different germ plates from different spore prints. All Golden Teacher. Thinly poured 20g agar 10g LME 1 liter. Thank you in advance.

Four plates, top and bottom. (1,2,3,4)






All banging plates in my opinion but id transfer 2 a little more to get the perfect round.....


Is it okay to go to grain even if its just the first transfer? For the ones that look good?


You can got to grains and new plates.  Just leave a little on the plate when you A2G, or transfer b4

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Edmunter]
    #26541280 -

This one seems to have a similar aerial fuzz in the middle as mine on the previous page, I’m guessing that is normal? If it were a contam I am guessing it would show black pin heads or change color before the mycelium reached the edge of the plate?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: orange771]
    #26541282 -

I love aerial fuzz.  Tilt the plate, it may actually be rhizo reaching for the heavens.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: orange771]
    #26541283 -

orange771 said:
This one seems to have a similar aerial fuzz in the middle as mine on the previous page, I’m guessing that is normal? If it were a contam I am guessing it would show black pin heads or change color before the mycelium reached the edge of the plate?


Contams grow faster as well...easy to spot based on their explosive growth compared to mush myc.


--------------------
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Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #26541390 -

Thanks, I started using a cork borer for transfers and switched to LME then it was so easy that I did 25 transfers off one plate. I was gonna be bummed if they were all bunk. To grain some will go!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Zakkery]
    #26541416 -

Zakkery said:
Anyone mind taking a look at the below pics? Some look completely fucked I know. Any advice on what you see I have done wrong would be appreciated. Im guessing mainly my swab/sterile technique could take a lot of practice.

I tried a couple runs before but believe I was making the agar too stiff and nothing was taking. This time round I have some growth at least but cant discern much given its my first go.

The plates are made with a 20g ag/20g lme/1l water. Some are swabbed, some are dropped from a syringe. These are all the plates showing some sort of growth. Is any of it useful for a transfer or should I keep working on my technique?




Looks you have plenty to work with, best would be 7, only looks like 1 large growth, 6 looks good too, just take a small transfer from outer edge... look at more pictures of good growth and compare :smile:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: C12ShroomMan]
    #26542652 -

So I don't really know what I'm doing with agar. I am on my second mono with more grains colonizing but I don't know how I should be going about my agar work. Do I just keep isolating strains? I've taken 2 prints and plated one clone, as well as starting a new round of isolations plus my first set of isolations that I have been working on for about 6 months. I feel like I'm just doing what I see other people on here do without having an end goal in mind. Need some guidance because my refrigerator is slowly filling with plates...

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Shroomwomb]
    #26542670 -

Shroomwomb said:
So I don't really know what I'm doing with agar. I am on my second mono with more grains colonizing but I don't know how I should be going about my agar work. Do I just keep isolating strains? I've taken 2 prints and plated one clone, as well as starting a new round of isolations plus my first set of isolations that I have been working on for about 6 months. I feel like I'm just doing what I see other people on here do without having an end goal in mind. Need some guidance because my refrigerator is slowly filling with plates...


What are you trying to achieve?

If you want to grow lots of shrooms, grow 1 MS grow, clone 3-4 mushrooms and test.  If its decent keep that culture, if not toss it.  Always freshen up your cultures and varieties and unless you are isolating for any scientific reason forget it.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Edmunter]
    #26542918 -

Yeah plates will build up super fast. I was just kind of floating around doing a ton of random shit too until recently I decided to just focus on getting one good clone worth keeping for each variety, and some I keep a clean ms plate also.

Gives you an end goal and doesn’t take forever, plus it’s super nice to have something reliable on deck. I’ve been doing ms grows to stock prints and take clones, then just doing clones. At first I was just stoked to have a bunch of prints for different kinds, but now I’m working on leveling each kind up to a stock of prints and a good clone culture filed away.

And then there’s you know....the piles of shrooms that accumulate along the way.


--------------------
LAGM2020

Edited by A.k.a (03/18/20 03:43 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #26543217 -

I have a couple transfers of AA+, and each one the wedges fluff up and then proceed to grow out. Are you saying that fluffing is a sign of contamination or bacteria?


--------------------

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Re: AGAR ENVY! (Anything and All things agar!) [Re: psycho_nauticus]
    #26543241 -

Not necessarily. It’s probably just the myc consuming the last bit of the wedge before it jumps off onto the new plate. Post a pic.


--------------------
Back once again with the ill behaviour



Links I like

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smoothcat]
    #26543380 -

Well I did no pours, these are looking from the bottom of the jar, and they’re about the size of a dime now. I couldn’t get the best straight on pictures because the light doesn’t do the same since it’s rounded glass, I may try doing those in better sunlight. They’re both about a little less than dime size.

Transfer 1: (That's a blade mark at the 4 'o clock position)



Transfer 2:


Edited by psycho_nauticus (03/18/20 07:51 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: psycho_nauticus]
    #26543690 -

They both look good from what’s visible, especially the first one.

I just dug around and found this pic of aa+ t2 plate.



We picked the same color lol.

I can’t even fathom how frustrating this hobby would be without smartphone pics and this site.


--------------------
LAGM2020

Edited by A.k.a (03/18/20 11:17 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a] * 1
    #26543770 -

These holy grails are pretty sick. :thumbup:


Albino cambo pin clone from a streak plate.


--------------------
Tek's I use

LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26544033 -

Yeah, they look good from underneath, nice clean uniform growth. These are T2. I’m just going to let them grow out to full size and colonize was the whole agar puck, and then inoculate with those since they are clean. I’m not going to be taking all the way to 5 or 7 transfers. I’m eager to get this to grain, and it’s clean itself anyway, so there shouldn’t be any problems, and I’ll be inoculating with these two.
I’ll try to get some pictures in the sunlight today to show you what the actual culture looks like in the jar.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: staytrippy420]
    #26544156 -

staytrippy420 said:
These holy grails are pretty sick. :thumbup:


Albino cambo pin clone from a streak plate.


I just cannot settle for anything less than a perfectly clear view of my agar. It's sorta the whole point. No-pour glass petris only.


--------------------
It's so easy to laugh
it's so easy to hate
It takes guts to be
gentle and kind
-the smiths

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #26544281 -

LadysKnight said:
... a perfectly clear view of my agar. It's sorta the whole point. No-pour glass petris only.


Yes, well if I had it down.
How do you PC your poured glass petris?
thanks... :hatsoff:


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #26544299 -

Inthepit said:
LadysKnight said:
... a perfectly clear view of my agar. It's sorta the whole point. No-pour glass petris only.


Yes, well if I had it down.
How do you PC your poured glass petris?
thanks... :hatsoff:


Pour from pot to plates on kitchen counter (at any temp)
Let plates cool, lids off.
Add lids, stack in groups of 5, wrap in foil.
PC for 20, let cool completely before moving off burner

Edit: also, I use jar rings and trivet, add just enough water to cover trivet.


--------------------
It's so easy to laugh
it's so easy to hate
It takes guts to be
gentle and kind
-the smiths

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Edited by LadysKnight (03/19/20 10:06 AM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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