footpath said:

Yeah. And to keep them away from cats and hamsters.

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footpath said:

Yeah. And to keep them away from cats and hamsters.

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footpath said:

As I understand it and have experienced:

Higher nutrient contents encourage tomentose (fluffy) growth.
Lower nutrient contents encourage rhizomorphic (ropy) growth.

I usually shoot for no more than 50/50 - 20g agar / 20g LME / 1L H2O.
My normal recipe is 20g agar / 14g LME / 1L H2O. It usually gets me rhizomorphic growth that doesn't race to the edge of the plate before I can catch it (I'll have a couple days sometimes where I don't get the chance to look at my cultivation stuff)

There are other factors that will cause different styles of growth, too - contaminants, environment, etc. But, in normal conditions, I've noticed the nutrient content to be the most impactful.

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tedoro said:

footpath said:

As I understand it and have experienced:

Higher nutrient contents encourage tomentose (fluffy) growth.
Lower nutrient contents encourage rhizomorphic (ropy) growth.

I usually shoot for no more than 50/50 - 20g agar / 20g LME / 1L H2O.
My normal recipe is 20g agar / 14g LME / 1L H2O. It usually gets me rhizomorphic growth that doesn't race to the edge of the plate before I can catch it (I'll have a couple days sometimes where I don't get the chance to look at my cultivation stuff)

There are other factors that will cause different styles of growth, too - contaminants, environment, etc. But, in normal conditions, I've noticed the nutrient content to be the most impactful.

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I'm putting all of this into my pipe and smoking it.

I've come to believe (only from reading) that, in order of priority:

ropey fast growth stimulated by:

1) low nute agar plates
2) shallow poured plates
3) stiffer pucks (less water)

But my ponderings currently are... I wonder if my huge initial spore drop, and way too much sugar creates a perfect situation for contams to intermesh with the mycelia.

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footpath said:

It's definitely possible. They won't mate, of course. But they will latch on to your culture and cause problems any time they get their opportunity.
I used to just put one drop on the middle of the plate and that worked totally fine for me until recently. Had to separate my cultures from some obvious contamination, but it was fairly easy to pick it out.
It's not terribly efficient to try to isolate from spores - agar anyhow. Unless you do what's known as a serial dilution - essentially just putting a small amount of spores into a lot of sterile water and swabbing a small amount onto a plate.
I'd put more effort into just finding a good clone.

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Quote:

Clone the best fruit from each jar, and then test all of these against each other, separately, under the environment you will be growing them under. This will solve all of the PROBLEMS associated with false matings between partially compatible, but non fruiting, matings!!! -RR

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So, it just seems like, just grow something, clone from a nice medium-sized cluster, eat it, and then you know if it's worthy.

Inthepit said:

footpath said:

It's definitely possible. They won't mate, of course. But they will latch on to your culture and cause problems any time they get their opportunity.
I used to just put one drop on the middle of the plate and that worked totally fine for me until recently. Had to separate my cultures from some obvious contamination, but it was fairly easy to pick it out.
It's not terribly efficient to try to isolate from spores - agar anyhow. Unless you do what's known as a serial dilution - essentially just putting a small amount of spores into a lot of sterile water and swabbing a small amount onto a plate.
I'd put more effort into just finding a good clone.

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Ok so many vectors here. Just a noob here trying to prioritize different approaches.
So:
1. Serial dilution, c10 mentioned that and his streaking pattern seems to get there.
2. But what I find contradicting

Quote:

Clone the best fruit from each jar, and then test all of these against each other, separately, under the environment you will be growing them under. This will solve all of the PROBLEMS associated with false matings between partially compatible, but non fruiting, matings!!! -RR

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So, it just seems like, just grow something, clone from a nice medium-sized cluster, eat it, and then you know if it's worthy.

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Quote:

I wouldnt worry about isolates at this point. But still, if you start from spores, streaking gives you lots of benefits. Gives you more to choose from and more organized growth

You could start with spores,  streak a plate, transfer a few colonies, grow out the t1 plates , transfer a healthy sector from as many as you like, repeat this process as many times as you want, till you get organized growth. Then make a LI or a2g to make some jars (or a grain master to g2g) , grow those out and fruit them, then clone the best looking fruits/clusters, and keep notes on potency

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ichugwindex said:

Is this bacterial?


transferred from this last night

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