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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Psicomb]
    #25963190 -

Psicomvb said:
I confess I still havent ever taken my own prints yet



Don’t feel bad, I only made a syringe once and then never used it lol.

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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Pastywhyte]
    #25963248 -

I confess I tried to get ape spores, from Nate, to germ on agar with no success. So I tried again last week but instead of just rubbing my inoculating loop on the swab I pulled a piece of the cotton swab off and streaked the plate with that, and to my surprise they germinated and are taking the plate over. The big problem I have is trying to find enough time to transfer some mycelium to a new plate. But I'm so happy that I got them to germinate, and thank you much mushroomnate.


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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: MH5109]
    #25963276 -

MH5109 said:
I confess I tried to get ape spores, from Nate, to germ on agar with no success. So I tried again last week but instead of just rubbing my inoculating loop on the swab I pulled a piece of the cotton swab off and streaked the plate with that, and to my surprise they germinated and are taking the plate over. The big problem I have is trying to find enough time to transfer some mycelium to a new plate. But I'm so happy that I got them to germinate, and thank you much mushroomnate.



This is what I always recommend people to do with my swabs! I use tweezers tho. I wrap like 8 pairs of tweezers in foil separately and put them in a jar for sterilization in the PC. Then I always have a sterile pair on hand when ever I need one :super: I feel like it cuts down on contams sooo much with swabs!

I keep meaning to write up a tek for this but then I always forget :facepalm3:


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The thing about deceit is, you don't know your being deceived...

The only thing I am sure about is that I know nothing - socrates

Believe nothing you hear and half of what u see.

Edited by Apc123 (04/29/19 09:33 PM)

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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Apc123]
    #25963289 -

I just take a swab and streak a half dozen plates or so. Speaking of, I have a pike of neglected plates and a pile of prepped grain... better PC it meow


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Never give up, never surrender.

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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Failboat]
    #25963318 -

I just soaked my tweezers in alcohol for 10min then flame sterilize, after they dry off a bit. Iv got some yeast and bacteria on the plates but that's the way all my germ plates usually end up. Iv just had a big hard on for some ape and it feels so good to see it starting, now I need to make the day a few hours longer or I need to tell my boss that I need to work less lol.


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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: MH5109]
    #25963527 -

Different tailors from them other dudes.


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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: NothingsChanged]
    #25964203 -

ive been using the tiger drop lately and always end up with a piece of agar stuck on the side of the jar  :foreheadslap:

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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: domK]
    #25964276 -

Apc123 said:
I use tweezers tho. I wrap like 8 pairs of tweezers in foil separately and put them in a jar for sterilization in the PC. Then I always have a sterile pair on hand when ever I need one :super: I feel like it cuts down on contams sooo much with swabs!



I just saw this technique, but for Agar->Grain/Sub. This dude just has a fucking jar full of them (at 9:54)


I'm probably going to start doing this, but likely in a few jars with a few tweezers in each.

domK said:
ive been using the tiger drop lately and always end up with a piece of agar stuck on the side of the jar  :foreheadslap:



Hold it steady with one hand in a standard vertical orientation. Smack it on the bottom with your other hand. It should slllllliiiiiide down the side into the grain eventually. Don't smack the jar into things, smack the jar with things while the jar stays as stationary as possible. :thumbup:

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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: ShaperDreaming]
    #25964311 -

I MUST CONFESS... I hope yall laugh but Look at my attempt to make a sab out of a dresser and saran rap

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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Himinji]
    #25964318 -

Wont work ... you dont have any plastic tubs laying around ? Or a big cardboard box ?


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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Boogieman47]
    #25964331 -

Boogieman47 said:
Wont work ... you dont have any plastic tubs laying around ? Or a big cardboard box ?



:lol: Could he line the whole thing with plastic to make a drawer work? :lol:

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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: ShaperDreaming]
    #25964345 -

Himinji said:
I MUST CONFESS... I hope yall laugh but Look at my attempt to make a sab out of a dresser and saran rap



Thats a good one! lol
I cant even imagine what kinda work could be done in a dresser drawer. Now you have to make that shit work just to say you did it.

I had a sigh of relief when I checked on things this morning, my beer agar seems to be working, one or 2 more days and I'll know for sure. Myc is beginning to jump off for sure. I was really worried I might not have cooked out the alcohol very good, or at all, and that would be a problem.
Tried all sorts of different recipes with and without food color too. I think it's time to pitch the food color, it was cool for a little bit but its just impractical. The closer I can get straight up CLEAR dishes the better.
Might just use beer agar from now on because it seems to work and the plates are sexy bitchez.

Edited by El Chupacabra (04/30/19 02:48 PM)

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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: El Chupacabra] * 1
    #25964361 -

I confess when I first saw the post I thought ok this will be bad ass and no one had thought of it before .. I thought he took a dresser and took the drawers out .. cut 2 arm holes into the back cut the top off .. then sealed with bisqueen however it's spelled that heavy duty plastic .. if you made some shelves that's how you could make a sab for bags and whatever else .. you could make shelves at different levels make doors for multiple levels of arm holes for different work that day .. you would be standing and hunched over though..


But that is what went through my head when I saw the I made a sab out of a dresser haha.. anyway I hope someone makes a box as I've described


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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Himinji]
    #25964370 -

Himinji said:
I MUST CONFESS... I hope yall laugh but Look at my attempt to make a sab out of a dresser and saran rap



You'll need more tape...:jah: :rofl:


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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Boogieman47] * 1
    #25964385 -

DRAWER CONTRAPTION INCOMING... lol its all in the name of seeing what i could get away with, will build a tub sab tho, ya know for success reasons

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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Himinji]
    #25965986 -

I confess, I did a bunch of agar work last night and when i finished up I managed to knock over the lamp while turning it off and it fell onto the table where all the plates I had just transferred were sitting, only one was cracked but a couple  of them ended up on the floor with the wedges knocked to the lid or side of the plate. :facepalm:


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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: AyePlus]
    #25966074 -

AyePlus said:
I confess, I did a bunch of agar work last night and when i finished up I managed to knock over the lamp while turning it off and it fell onto the table where all the plates I had just transferred were sitting, only one was cracked but a couple  of them ended up on the floor with the wedges knocked to the lid or side of the plate. :facepalm:



Been there, done similar :heart: It's tough to fuck up a couple hours of work with a misplaced movement.

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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: ShaperDreaming]
    #25966088 -

domK said:
ive been using the tiger drop lately and always end up with a piece of agar stuck on the side of the jar  :foreheadslap:



Hold it steady with one hand in a standard vertical orientation. Smack it on the bottom with your other hand. It should slllllliiiiiide down the side into the grain eventually. Don't smack the jar into things, smack the jar with things while the jar stays as stationary as possible. :thumbup:



right on ill give that a go next time

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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: domK]
    #25966149 -

On the really stubborn wedges that won't budge you can lay your jar on its side until you get leap off.


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When you clean a vacuum cleaner you are a vacuum cleaner
Build yourself a Flow Hood in these 99 simple steps

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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Grimsweeper]
    #25966155 -

Grimsweeper said:
On the really stubborn wedges that won't budge you can lay your jar on its side until you get leap off.



This too! I've totally done that lol... or rather 45 degree angles :rofl:

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