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OfflineLegend21
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Failboat]
    #25945293 - 04/20/19 08:42 AM (4 years, 9 months ago)

Quote:

Quirkmeister92 said:
I have noticed trash in cheap brands. I doubt your tent is causing the problem. There's already spores in the air and you have lids on your tubs so how much of that exhaust is really making it into you tubs? I expect this is not the trouble. Was your grain prepared in one PC session?




Due to the set up of the room the exhaust is blowing right at my tubs although about six seven feet
away. So could be quite a bit but I doubt it to. Just trying to brainstorm.

Id say the majority of them were pc around the same time but not all of them. I guess that could be a source also. I guess I’ll see these new seven were all different jars and different cultures.


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Offlineverum subsequentis
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Legend21]
    #25945425 - 04/20/19 10:15 AM (4 years, 9 months ago)

Having lids on your tubs will not stop trich spores from entering. I'm not saying that the tent exhaust is the problem but it certainly isn't helping. If i were growing cannabis in the same room that i was fruiting cubes, I'd definitely be growing in coir.


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OfflineLegend21
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: verum subsequentis]
    #25945520 - 04/20/19 11:14 AM (4 years, 9 months ago)

Quote:

verum subsequentis said:
Having lids on your tubs will not stop trich spores from entering. I'm not saying that the tent exhaust is the problem but it certainly isn't helping. If i were growing cannabis in the same room that i was fruiting cubes, I'd definitely be growing in coir.





Yeah I hate growing in coco. Then you have to use chemical fertilizers most of the time and many of those contain trich also. I might just keep messing around and if it keeps happening I’ll shut the tent down. I like growing shrooms more these days anyway.


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InvisibleMadHatter333
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Legend21]
    #25945707 - 04/20/19 01:10 PM (4 years, 9 months ago)

I confess it’s been a rough five days. Not to put it on blast, but for everyone who was empathetic to my struggle Here it is


--------------------

TEKs I Like


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InvisibleYesum
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: MadHatter333]
    #25946894 - 04/21/19 05:21 AM (4 years, 9 months ago)

The coir could be your culprit too. Sometimes bucket teching doesn't work well enough. Just saying. If you have jars your sure are clean. And your still getting moldy tubs. Try sterilizing the coir. Or at least do something to keep the temp inside your bucket up for longer. Or oven roasted coir bricks prior to bucket teking is proving to be a valuable added step.


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OfflineLegend21
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Yesum]
    #25946906 - 04/21/19 05:47 AM (4 years, 9 months ago)

Quote:

Yesum said:
The coir could be your culprit too. Sometimes bucket teching doesn't work well enough. Just saying. If you have jars your sure are clean. And your still getting moldy tubs. Try sterilizing the coir. Or at least do something to keep the temp inside your bucket up for longer. Or oven roasted coir bricks prior to bucket teking is proving to be a valuable added step.




Yeah that’s what I first blamed because I switched coir brands. But I just spawned seven tubs with my old brand of coir just trouble shooting as I go. Now I’ll see if maybe it was the coir or not. If all of these new tubs get it also I’ll have to keep trouble shooting.


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InvisiblePastywhyteMDiscord
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Legend21] * 1
    #25947061 - 04/21/19 08:13 AM (4 years, 9 months ago)

IMO it’s not the bucket that’s the problem, the problem is that some brands of coir...have more than just coir in them. If there are a lot of seeds or woody debris in the coir brick, those things can contam if not colonized sterile. Proper pasteurization won’t do jack to help with that issue either.

So if the bucket fails you and you suspect it’s debris or seeds in the brick, you need to colonize that shit in vitro.


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OfflineLegend21
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Pastywhyte]
    #25947099 - 04/21/19 08:46 AM (4 years, 9 months ago)

Quote:

Pastywhyte said:
IMO it’s not the bucket that’s the problem, the problem is that some brands of coir...have more than just coir in them. If there are a lot of seeds or woody debris in the coir brick, those things can contam if not colonized sterile. Proper pasteurization won’t do jack to help with that issue either.

So if the bucket fails you and you suspect it’s debris or seeds in the brick, you need to colonize that shit in vitro.




Well I had the opposite experience as you I was just reading through your thread the other day. I seem to have good luck with eco earth and bad luck with the exo terra. I guess they all have shitty quality control though.


How would you colonize invitro with coir?


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InvisibleSunnyDayze
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Legend21]
    #25947184 - 04/21/19 09:46 AM (4 years, 9 months ago)

Drives me nuts how bad my memory is but someone, a TC or someone that I trust advice from, said that they put their coir bricks in the oven for a couple hours at 200 f (something like that, shit memory wish I wrote it down) and then bucket tek.

Would this help kill unwanteds Pasty?


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OfflineLtLurker
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: SunnyDayze] * 1
    #25947191 - 04/21/19 09:51 AM (4 years, 9 months ago)

I've seen after bucket hydrating quite a bit, and only seen before bucket mentioned, but haven't seen it done.

Most ovens go down to 170, pastuerizing temps are 150-170. If I put it in when I hydrate it, it only takes about 2-3 hours and works fine. If you put it in the oven cold, you're looking at 5 hours.

And yes it works for me so far. My coir went from molding by itself in a week, to sitting around just fine like it's supposed to.

/e @legend, colonizing invitro would be something like a Muda Bottle where you sterilize all in one.


Edited by LtLurker (04/21/19 10:07 AM)


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OfflineLegend21
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: LtLurker]
    #25947230 - 04/21/19 10:13 AM (4 years, 9 months ago)

Quote:

LtLurker said:
I've seen after bucket hydrating quite a bit, and only seen before bucket mentioned, but haven't seen it done.

Most ovens go down to 170, pastuerizing temps are 150-170. If I put it in when I hydrate it, it only takes about 2-3 hours and works fine. If you put it in the oven cold, you're looking at 5 hours.

And yes it works for me so far. My coir went from molding by itself in a week, to sitting around just fine like it's supposed to.

/e @legend, colonizing invitro would be something like a Muda Bottle where you sterilize all in one.




So basically like a big PF cake with coco. F that :lol: I am going to try some Pan Cakes soon though.

I use bods bucket tek with the cooler and never had problems I think it’s just the switch to the different brand of coir.


So you hydrate and then oven bake?


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OfflineLtLurker
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Legend21]
    #25947244 - 04/21/19 10:19 AM (4 years, 9 months ago)

yea, instead of a bucket I use a big pot, boil water add coir, let it saturate for like an hour, mix, into oven. 3/3 so far, before this i was doing individual batches for each tub, took longer tho.

I tried switching coir from the big garden bricks to eco earth, but I was still having the same problem.


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OfflineLegend21
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: LtLurker]
    #25947292 - 04/21/19 10:43 AM (4 years, 9 months ago)

Quote:

LtLurker said:
yea, instead of a bucket I use a big pot, boil water add coir, let it saturate for like an hour, mix, into oven. 3/3 so far, before this i was doing individual batches for each tub, took longer tho.

I tried switching coir from the big garden bricks to eco earth, but I was still having the same problem.




That’s cool so you bring it to field capacity before or after the oven? I saw you saturate it didn’t know if that’s just to fluff it up I’ll try that.


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OfflineLtLurker
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Legend21]
    #25947317 - 04/21/19 10:52 AM (4 years, 9 months ago)

roughly field capacity. I treat it just like you would normally do bucket tek, then adding extra heating at the end before it cools down. It may need some water or squeezing if the water content is a little off at the end.


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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: LtLurker] * 1
    #25947343 - 04/21/19 11:01 AM (4 years, 9 months ago)

Colonized invitro. and fruited in a tub.


And all winter, with every culture I had I'd get moldy tubs prior to first flush.

So after doing something like what pasty said.(colonizing invitro)And thinking it was just about everything under the sun. From my culture work to my grain prep to even my tap water being the culprit. after all of that. I may be finding out all I had to do. At least with this particular batch of coir I have (exo terra) is sterilize my coir after the bucket tek. Experiment is still underway.  Looking good so far though.


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InvisibleAyePlusS
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Yesum]
    #25947355 - 04/21/19 11:07 AM (4 years, 9 months ago)

I would only do in-vitro for making super clean prints, otherwise this hobby consumes enough plastic IMO. I’m already brainstorming ways to use monotubs when I’m done growing actives, top of the list right now is raising mealworms/crickets and storing fresh gourmet mushrooms. I lol every tine I see someone post on Facebook and their freshly harvested oysters are sitting in a mono. I think I’ve even seen people who have no idea what a mono is copying the design for storage because they saw someone else doing it. Actually works pretty good to keep them from getting soggy.


--------------------
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InvisibleYesum
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: AyePlus]
    #25947370 - 04/21/19 11:14 AM (4 years, 9 months ago)

No u don't fruit invitro.  You colonize your substrate invitro. once its colonized treat it like anything else

Using plastic is like u say the downside.

By doing that you can troubleshoot what's going on. Where the vector is you need to address

I like getting super clean prints from my plates that fruit. :thumbsup:


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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Yesum]
    #25947373 - 04/21/19 11:19 AM (4 years, 9 months ago)

Yesum what's your spawn ratio ? I am doing 1 to 4 right now it's taking 5 days to fully colonize give or take if I mixed the bag good enough


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InvisibleAyePlusS
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Yesum]
    #25947376 - 04/21/19 11:21 AM (4 years, 9 months ago)

I think you’re misunderstanding, I’m saying I would only fruit invitro to get the cleanest prints possible. Doing bulk in tubs is just too easy to colonize invitro and then move it to a tub to fruit, I guess the main benefit there is no need to clean tubs.


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
🅂 🄰 🄼 🄴  🅃 🄴 🄰 🄼
🅃 🄴 🄰 🄼  🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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InvisibleYesum
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: AyePlus]
    #25947400 - 04/21/19 11:30 AM (4 years, 9 months ago)

Yeah it's easy to clean. There is no benefit to it really. Aside from being able to micro manage eash step a little more to better understand what's going wrong if and when moldy tubs start showing up.

I'm not exactly sure. Probably close to a 1:1

I throw  like 4 large handfuls of coir into a bag and use 1 quart jar of colonized wbs. So it's probably on the high side. I could probably inoculate 2 bags with 1 jar. Colonizing is quick though. Like 5 days. Give or take.


Oh there is one plus side. I've used jars that were only a little more then half colonized to inoculate a bag. (In front of my flowhood) so you could add speed to the operation if you wanted to.


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