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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
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Last seen: 3 days, 9 hours
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Bd5784] * 1
    #25941697 - 04/18/19 02:08 PM (4 years, 9 months ago)

Quote:

Bd5784 said:
for sanitizing jars, arms, workspace, Inside of sab before mist. First spray with half H2o2 half water couple drop of soap ( I use dr bronners  peppermint) then soak a rag with white vinegar and wipe everything down.  It doesn’t evaporate near as fast ( giving longer germ killing) and you don’t breath a bunch of alcoholvapors if working in a small space.  There’s an article on here somewhere about it.  Seems like more work but I think it’s better and less toxic.



None of Those things sanitize


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineBd5784
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Registered: 10/14/18
Posts: 172
Last seen: 2 years, 4 months
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: tryptkaloids]
    #25941828 - 04/18/19 03:33 PM (4 years, 9 months ago)

“It doesn't matter which you use first - you can spray with the vinegar then the hydrogen peroxide, or with the hydrogen peroxide followed by the vinegar. You won't get any lingering taste of vinegar or hydrogen peroxide, and neither is toxic to you if a small amount remains on the produce.

As a bonus: The paired sprays work exceptionally well in sanitizing counters and other food preparation surfaces -- including wood cutting boards.

In tests run at Virginia Polytechnic Institute and State University, pairing the two mists killed virtually all Salmonella, Shigella, or E. coli bacteria on heavily contaminated food and surfaces when used in this fashion, making this spray combination more effective at killing these potentially lethal bacteria than chlorine bleach or any commercially available kitchen cleaner. ”
  Works great for me
Here’s the linkhttps://www.shroomery.org/forums/showflat.php/Number/2170062


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OfflineShaperDreaming
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Registered: 10/30/18
Posts: 3,429
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Bd5784] * 1
    #25941836 - 04/18/19 03:36 PM (4 years, 9 months ago)

Quote:

Bd5784 said:
“It doesn't matter which you use first - you can spray with the vinegar then the hydrogen peroxide, or with the hydrogen peroxide followed by the vinegar. You won't get any lingering taste of vinegar or hydrogen peroxide, and neither is toxic to you if a small amount remains on the produce.

As a bonus: The paired sprays work exceptionally well in sanitizing counters and other food preparation surfaces -- including wood cutting boards.

In tests run at Virginia Polytechnic Institute and State University, pairing the two mists killed virtually all Salmonella, Shigella, or E. coli bacteria on heavily contaminated food and surfaces when used in this fashion, making this spray combination more effective at killing these potentially lethal bacteria than chlorine bleach or any commercially available kitchen cleaner. ”
  Works great for me
Here’s the linkhttps://www.shroomery.org/forums/showflat.php/Number/2170062




That's 15 year old information dood


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OfflineSpindlymass
wafer thin.
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Registered: 09/25/17
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: ShaperDreaming] * 2
    #25942010 - 04/18/19 04:57 PM (4 years, 9 months ago)

I confess,

It's nice to have enough experience to think for myself now and not just blindly follow teks.

Hallelujah for teks and I still don't know shit, but the comfort experience brings feels good.

:coolcat:


--------------------
enough knowledge to get into trouble. Not nearly enough to get out again.


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OfflineBd5784
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Registered: 10/14/18
Posts: 172
Last seen: 2 years, 4 months
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: ShaperDreaming]
    #25942070 - 04/18/19 05:37 PM (4 years, 9 months ago)

Ok y’all can hate but it works for me every time. H2o2 and vinegar. I don’t like breathing nasty fumes of iso.


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OfflineLegend21
Legend
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Registered: 04/27/18
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Bd5784]
    #25942073 - 04/18/19 05:38 PM (4 years, 9 months ago)

I confess I used a plastic inoculation loop dipped in iso to swipe P. Galindoi spores to agar. Time to see if the alcohol is enough :rofl:


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OfflinePsiloPsychIn
PsiloPsychIn
I'm a teapot User Gallery


Registered: 06/17/14
Posts: 8,182
Loc: up north
Last seen: 3 days, 14 minutes
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Bd5784] * 3
    #25942116 - 04/18/19 06:00 PM (4 years, 9 months ago)

Quote:

Bd5784 said:
“It doesn't matter which you use first - you can spray with the vinegar then the hydrogen peroxide, or with the hydrogen peroxide followed by the vinegar. You won't get any lingering taste of vinegar or hydrogen peroxide, and neither is toxic to you if a small amount remains on the produce.

As a bonus: The paired sprays work exceptionally well in sanitizing counters and other food preparation surfaces -- including wood cutting boards.

In tests run at Virginia Polytechnic Institute and State University, pairing the two mists killed virtually all Salmonella, Shigella, or E. coli bacteria on heavily contaminated food and surfaces when used in this fashion, making this spray combination more effective at killing these potentially lethal bacteria than chlorine bleach or any commercially available kitchen cleaner. ”
  Works great for me
Here’s the linkhttps://www.shroomery.org/forums/showflat.php/Number/2170062




I'll start with this... I confess the following will sound like I'm a know it all. I am still a noob, but I do know a few things about this hobby...

Although the quoted passage is about cleaning fruit, the earlier discussion was about cleaning SABs and I hear the tone of the discussion in here and I have to say, I've been biting my tongue.
There are a few things I think you should consider.
One, vinegar (at least the active / alive culture variety) has its very own culture that could take hold on agar and grains. So while it may discourage the growth of many things (partially sanitize) it may grow where you don't want it to.
Two, just because something "works" in mycology doesn't necessarily mean it is the most effective or reliable. You can do open air inoculations and get lucky .. even get lucky fairly often. That doesn't mean it is just as good a procedure as an SAB.
Three, the effectiveness of an SAB does not depend a lot upon how clean it is inside. It depends most on how "Still" the air is inside there and your sterile technique which is about how little you expose your sterile materials to falling particulates.
A very simple tech that avoids the "toxic" alcohol (an organic byproduct of yeast metabolism by the way), is simply to wipe down the inside of your SAB with slightly soapy water (I love doctor bronner's) and then to spray a fine mist all around the inside about 10 minutes prior to doing your work. This does a pretty good job of cleaning surfaces (the soap breaks surface tension and makes the water more effective and collecting spores etc.) and making particulates (most not all) drop out of the air.
Slow mindful deliberate motions (that do not stir up much air current) in your SAB is probably the single most important part of the technique.

As for inoculation loops and scalpels - I would not clean those in vinegar for the reasons I mentioned above and highlighted below:

If you are going to cleanse with alcohol Doc... 70% is better than 90% because it tricks germs into letting the alcohol to cross the cell walls due to the presence of higher water content. Alcohol will do a pretty good job and is a reasonable short cut for not flaming your tools, but see point two above.

https://survivallife.com/make-vinegar-mothers-milk/

https://www.researchgate.net/publication/249362167_Isolation_and_characterization_of_vinegar_culture_Acetobacter_aceti_from_indigenous_sources


--------------------
What are they saying? Listen carefully, it might be something you need to hear...





Edited by PsiloPsychIn (04/18/19 06:07 PM)


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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 8 hours, 14 minutes
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Legend21]
    #25942134 - 04/18/19 06:09 PM (4 years, 9 months ago)

Quote:

Legend21 said:
I confess I used a plastic inoculation loop dipped in iso to swipe P. Galindoi spores to agar. Time to see if the alcohol is enough :rofl:




Galindoi
Did these with a qtip. Run em through the PC wrapped in foil. Nice n easy


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Failboat]
    #25942326 - 04/18/19 07:54 PM (4 years, 9 months ago)

I worked a dry SAB without gloves or iso for months... none of that shit is necessary with proper sterile technique and thoughtful hand/fingertip placement (avoiding jar/dish lid rims, placing more of your instruments or self over receiving media than needed to do work)... I set up SAB, wash my hands from elbow out(usually... ok, sometimes... alright alright, hardly ever) and go to work using slow concise movements... with laminar flow,  I glove and mask up (new), flip a switch, press play on the headphones/YouTube playlist, and get to it... honestly, I don't even give much thought to how I move;  hover over open plates and take pictures and shit... love it

I confess, I cannot WAIT to do some bag2bag G2G in front of this bitch!



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OfflineDnDRnD
Hobby cultivator


Registered: 10/08/18
Posts: 2,906
Loc: Washington Flag
Last seen: 7 months, 23 days
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
    #25942337 - 04/18/19 07:58 PM (4 years, 9 months ago)

Quote:

Caps McGee said:
I worked a dry SAB without gloves or iso for months... none of that shit is necessary with proper sterile technique and thoughtful hand/fingertip placement (avoiding jar/dish lid rims, placing more of your instruments or self over receiving media than needed to do work)... I set up SAB, wash my hands from elbow out(usually... ok, sometimes... alright alright, hardly ever) and go to work using slow concise movements... with laminar flow,  I glove and mask up (new), flip a switch, press play on the headphones/YouTube playlist, and get to it... honestly, I don't even give much thought to how I move;  hover over open plates and take pictures and shit... love it

I confess, I cannot WAIT to do some bag2bag G2G in front of this bitch!






God i need to get a hood, I'm jelly of the freedom when working they provide


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662

Down with the bourgeoisie and up with the proletariat


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: DnDRnD]
    #25942344 - 04/18/19 07:59 PM (4 years, 9 months ago)

Yessir... it's... fucking cheating really lol


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OfflineApc123
Mushroom momma
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Registered: 04/26/18
Posts: 1,243
Loc: US Flag
Last seen: 1 year, 3 months
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
    #25942357 - 04/18/19 08:04 PM (4 years, 9 months ago)

I confess, I cannot WAIT to do some bag2bag G2G in front of this bitch!






:wtfsonic: omg I'm so jelly! Hahaha apparently I'm not the only one :lol:


Edited by Apc123 (04/18/19 08:05 PM)


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OfflineDnDRnD
Hobby cultivator


Registered: 10/08/18
Posts: 2,906
Loc: Washington Flag
Last seen: 7 months, 23 days
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Apc123]
    #25942431 - 04/18/19 08:42 PM (4 years, 9 months ago)

Hell yeah, ive always had an idea of like a giant hood attached to the side of a shed to create a laminar flow room sort of thing where the whole room is one big ass sterile environment


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662

Down with the bourgeoisie and up with the proletariat


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OfflineAsuraS
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 22 hours
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Apc123]
    #25942434 - 04/18/19 08:42 PM (4 years, 9 months ago)

What a size queen! :lol:

My little hood seems so inadequate now.


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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 8 hours, 14 minutes
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Apc123]
    #25942471 - 04/18/19 08:59 PM (4 years, 9 months ago)

Quote:

Apc123 said:
I confess, I cannot WAIT to do some bag2bag G2G in front of this bitch!






:wtfsonic: omg I'm so jelly! Hahaha apparently I'm not the only one :lol:</font>



:whatshesaid: I'm done putting it off, I'm calling the filter manufacturer tomorrow...


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OfflineBd5784
 User Gallery


Registered: 10/14/18
Posts: 172
Last seen: 2 years, 4 months
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: PsiloPsychIn]
    #25942509 - 04/18/19 09:21 PM (4 years, 9 months ago)

First off just because something is organic does not mean it is non toxic. And white vinegar does not contain the mother, so no worry of inoculating the non existent yeast from the vinegar onto plates. Thank you for your perspective though, I was not trying to convince anyone my way is the best, just confessing it’s my way of getting things done. IT is effective for me, I have lung issues and breathing in the alcohol from the saturated paper towel and jars made them worse, also why I switched to a propane torch instead of an alcohol lamp a much cleaner working space. But don’t knock it till you try it. You can theorize all day and say oh everyone does this because it’s the most efficient and what not.  H2o2 kills spores. Vinegar kills bacteria and fungi molds etc. in combination togeather it makes a great sanitizer imo.


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InvisibleNothingsChanged
Striving for Excellence
Male User Gallery

Registered: 05/28/11
Posts: 10,146
Loc: North/Western WA Flag
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Bd5784]
    #25942542 - 04/18/19 09:45 PM (4 years, 9 months ago)

Yo B, just keep doing you. It's all good. What ever works for you. :hug: Dropping school names and tech institutes does not make you any better.We are all the same. Not just here.


--------------------


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 9 hours
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Bd5784]
    #25942558 - 04/18/19 09:57 PM (4 years, 9 months ago)

Quote:

Bd5784 said:
Ok y’all can hate but it works for me every time. H2o2 and vinegar. I don’t like breathing nasty fumes of iso.



As said before, just because you got the desired result doesn't mean vinegar sanitizes. I clean my sab 2-4 times a year and very rarely mist. Yet my agar work is at about 95% success. Its all in the technique. Use a little water and soap. Forget the vinegar and h202. Theu are amazing for household use, but have no use in the hobby


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineEl Chupacabra
Stoned Ape
Male


Registered: 01/07/19
Posts: 676
Loc: 🇺🇸
Last seen: 1 year, 17 days
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: tryptkaloids]
    #25942605 - 04/18/19 10:31 PM (4 years, 9 months ago)

I confess Ive been smoking bud nearly everyday for about 24 years and I recently stopped. It's been about 2 weeks now. It's not because I don't want to smoke or anything like that, it's because I'm frustrated with the quality decline in my area and the prices have remained the same.
I'm going on vacation in about a month to Colorado and I'm pretty excited for it.

It was pretty hard to adjust at first but I think my temperament has normalized finally.


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OfflineBd5784
 User Gallery


Registered: 10/14/18
Posts: 172
Last seen: 2 years, 4 months
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: tryptkaloids]
    #25942610 - 04/18/19 10:34 PM (4 years, 9 months ago)

Ok 👌 cool guess I’ll just keep my confessions to myself.  And now the thread goes on...


Edited by Bd5784 (04/18/19 10:40 PM)


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