Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

The Spore Depot
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   North Spore Cultivation Supplies   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Psychedeliciouss]
    #24836907 -

If you wrap them with parafilm and store them in something airtight, E.G a ziplock bag or a tupperware, should be fine. Would also show if your technique isnt good considering any plates that would contam due to tek would do so over a month.


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: ManifoldPrime]
    #24836909 -

ManifoldPrime said:
Would also show if your technique isnt good considering any plates that would contam due to tek would do so over a month.


Hes talking about storing cultures in the fridge


--------------------
Cakes inside Water Tub with optimal Surface Conditions

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: ManifoldPrime]
    #24836986 -

thank you :smile:

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Psychedeliciouss]
    #24889755 -

Thanks for this!

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Snickelfritz]
    #24895955 -

Great read booked for later studies


--------------------
Spore Collection
Thai Lapi Yai 2018 end of year grow

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: mushboy]
    #24913146 -

This is awesome!  Thx for the details:thumbup:

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Pablo1211]
    #24941070 -

Gonna be getting into agar, and I especially liked the streaking technique, gonna use that, Thanks for the experienced info.

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #24950258 -

Thank you for this. First time grower here and decided to start with spores on agar. This has been my bible and hasn't failed me yet.

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: w33dh4x]
    #25116373 -

c10h12n2o, I think I just found my hero.
Started a couple grain bags and some BRF jars a week ago. To keep me occupied while they colonize, I decided to jump straight into agar for hands on experience and put some use to my left over syringe.
Learned a few things right off the bat; My 64q SAB is to short. My petri stand to keep space between the cloth on the bottom and my petri dishes is too high along with my 1000ml slimline media bottle.
Gonna have to make some adjustments, thinking a home made plexi glass one may be the way too go. Next step, knock one up and see how my other dishes grade my sterile technique. "Like a blind man in an orgy ya gotta feel things out", I guess.

Great write up, fuel to my newfound fire, thank you for sharing! :thumbup:


--------------------
-The heaviest thing one will ever carry is a thought-
-"Like a Blind man In an orgy you gotta feel things out.".-
-When we agree about our hallucinations, we call it “reality".-
-If you defy authority because your told to, that's no better than blindly trusting authority.-

psychonautwiki.org

How it should & shouldn't look - NEW CULTIVATORS GUIDE
BOD's Easy AF OAT prep tek.
Principles of mushroom growing for beginners

Edited by R.I.P.Zappa (04/05/18 04:59 PM)

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #25149190 -

C10,
First of all let me praise you for the clear and concise TEK you’ve provided and let you know that I realize, how much time and effort you’ve put into doing it. Just amazing!

Now, first an excuse:  I’m (as of this coming June) 80 years old.  I’ve just started cultivating psilocybe cubensis in the last few months and I’m having a difficult time navigating the Shroomery board and absorbing all the information available.

I’ve spent the last several hours reading your TEK and a number of others. At this point I seem to be in overwhelm  I’ve finished PCing a few agar dishes using Gladware plastic mini-containers and I have a spore print ready to use.

Here’s my question: What’s the best method of getting the spores from the spore print onto the agar?  I don’t seem to find in your TEK how you’re transferring the spores onto the agar.  It’s probably just me.  I’ve started to slow down in my comprehension these last couple of years.  Are you just using an inoculating loop to scrape some spores from the print?

There’s no need to write something out and waste your time. If you could just point me to the right post.  I’ve tried searching and just haven’t been able to come up with a clear procedure.

I noticed that somewhere in your description you mention not to drop any liquid from a syringe onto the petri dish as that just leads to contamination and spreading the mycelium growth indiscriminately across the agar.

Any advice or suggestions you might provide would be greatly appreciated.

Thanks in advance,
JB

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: bison2] * 1
    #25149503 -

These may help. Gotta do allot of digging to fill in the blanks. Sometimes ya find the answer to what you are looking for in a thread not even related to what you are looking for. Good thing is you never run out of stuff to read and often find somthing new.

Here is a good one for how to use the search engine better.
https://www.shroomery.org/forums/showflat.php/Number/24270830#24270830

How to use the sites features
https://www.shroomery.org/forums/showflat.php/Number/23745550

Heres is a bunch of compiled agar thread links.
https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954

Simple example:
I copy pasted your, "spore print onto the agar", from your sentence,
"Here’s my question: What’s the best method of getting the spores from the spore print onto the agar?", onto the search bar on the top and came up with this.
https://www.shroomery.org/forums/showflat.php/Number/20296483#20296483

Could also ask in these two threads as well.
https://www.shroomery.org/forums/showflat.php?Cat=&Board=83&Number=25145624&fpart=1&PHPSESSID=
https://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=24679216&fpart=1&PHPSESSID=

There is more than one way to skin a cat and there is allot of info and helpful folks here.
Welcome and :goodluck:


--------------------
-The heaviest thing one will ever carry is a thought-
-"Like a Blind man In an orgy you gotta feel things out.".-
-When we agree about our hallucinations, we call it “reality".-
-If you defy authority because your told to, that's no better than blindly trusting authority.-

psychonautwiki.org

How it should & shouldn't look - NEW CULTIVATORS GUIDE
BOD's Easy AF OAT prep tek.
Principles of mushroom growing for beginners

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: R.I.P.Zappa]
    #25255177 -

Nice! just chiming in to track this thread. :mushroom2:

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: k5hd2y]
    #25255235 -

this thread is dead for the most part. c10 hasn't been around for a while. it's a good right up though


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #25465191 -

Very detailed,,thanks for taking the time to share it.
Happy I found this post, will bookmark it for sure.


--------------------
:protest:
! When the system you live in has destroyed all the fun, the only fun left is to destroy the system !

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Casi Loco]
    #25466229 -

Super helpful write up c10h12n2o, great to see so many pix

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Scrubbs]
    #25534235 -

thanks for sharing! Great wealth of information there :smile:


--------------------

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #25565207 -

How can I utilize the agar dishes to inoculate PF cakes ?


--------------------
:protest:
! When the system you live in has destroyed all the fun, the only fun left is to destroy the system !

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Casi Loco]
    #25566225 -

this thread is DEAD my friend. agar to brf cake is not a normal way of doing this but you could (plausbly) just put it on top with no verm layer. better yet would be using clean agar to make a clean lc and then noccing up the cake with the lc. or... make an LI out of the agar and use that. bottom line though is brf cakes are for beginners who are NOT using agar. there is nothing wrong with still using brf cakes but grain spawned to bulk will give you better yields. brf cakes are for people who are still just using spores. spore syringes aren't all to clean and brf cakes are really good at mitigation bacterial infection.

... get yourself some wild bird seed (or other grain) and noc that shit up proper. just my two cents.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Casi Loco]
    #25566682 -

Casi Loco said:
How can I utilize the agar dishes to inoculate PF cakes ?


Make a cake with a grain jar lid (has filtered gas exchange), skip the dry verm barrier since your lid is now the filter. Then inoculate it like a grain jar.

Now for me to sound mean but if you have to ask i would stick to the beginner methods, get feet wet. And when all these terms and techniques make sense and are not so overwhelming and confusing then its time to branch to "advanced" methods.


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

Extras: Filter Print Post Top
Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: bodhisatta]
    #25567006 -

Or fuck it, do everything at once. It can work. :cool:


--------------------




ATTACK OF THE KILLER APE!!!11 | G2G Video | Stare Sticks | Bags in SAB |
DMT + Laser = Reveals Alien Code Commonly Reported In Trips | JavaScript I Use To Make Shroomery Better [New]

Extras: Filter Print Post Top
Jump to top Pages: | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |  [ show all ]

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   North Spore Cultivation Supplies   Mushroom-Hut Liquid Cultures

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Re: sterilizing tips/tricks -- please suggest some Anonymous 1,298 1 04/01/00 12:50 AM
by Anonymous
* some stealthy grow questions, any tips? absentminded 1,103 4 08/05/03 01:45 PM
by shakta
* Question about agar supplies Chemical_Smile 1,669 3 04/20/02 05:50 AM
by babyshroom
* Post Your New Tools Of The Trade.
( 1 2 3 4 ... 722 723 )		 hamloafM 460,795 14,444 08/06/26 01:57 PM
by ReverendMyc
* How it should & shouldn't look - NEW CULTIVATORS GUIDE
( 1 2 3 4 ... 87 88 )		 TmethylM 983,615 1,747 09/02/25 11:42 PM
by blabbalot
* c10's Flow Hood Build
( 1 2 3 all )		 c10h12n2o 45,027 53 08/31/21 03:07 PM
by sandman420
* c10's agar LME, is this the right LME? AlexSmitt 2,418 11 08/29/17 07:20 PM
by AlexSmitt
* Re: Use agar and break free from spore vendor dependency forever for only $10!
( 1 2 3 all )		 Anonymous 7,987 41 02/04/01 12:43 PM
by puscle

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
116,873 topic views. 12 members, 955 guests and 106 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.006 seconds on 16 queries.