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 ItsScience
a dude
Registered: 12/12/12
Posts: 101
Last seen: 9 years, 1 month
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 Re: Post Your New Tools Of The Trade.  [Re: camplo]
#21382229 - 03/09/15 11:29 AM (9 years, 10 months ago) |
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Quote:
 [/url]
All glass 100ml syringe, No more disposables, yuck!
I've been looking at glass syringes with luer lock tips, how does it work beyond being an autoclave dream?
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-SCIENCE
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liquidclutter
Stranger
Registered: 03/06/15
Posts: 161
Last seen: 9 years, 10 months
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Re: Post Your New Tools Of The Trade. [Re: ItsScience]
#21382242 - 03/09/15 11:32 AM (9 years, 10 months ago) |
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wut?
plastic syringes pc well many times
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Chk
Reverser
 
Registered: 09/06/13
Posts: 405
Loc: France
Last seen: 1 month, 12 days
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Re: Post Your New Tools Of The Trade. [Re: Dekozn]
#21382326 - 03/09/15 11:53 AM (9 years, 10 months ago) |
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My SHIP are made of silicone (like many people i guess), so it doesn't really matter if i have to redo it after 2 or 3 uses
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Fexus Boney Hadger
I care bigtime ®©™√
Registered: 12/23/09
Posts: 24,409
Loc: Spicemaster General.
Last seen: 1 hour, 30 minutes
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Re: Post Your New Tools Of The Trade. [Re: tourrat]
#21385098 - 03/10/15 12:16 AM (9 years, 10 months ago) |
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Quote:
tourrat said:
Ahhh...I just grasp the concept of the innoc gun...I think. Its just an inline pump essentially. Correct? I was wondering before with someone would want something that small(the cylinder) but now I see it looks like the tube goes in the jar of lc and the gun works as a pump. If so that is BAD ASS!! I have to have one of those! Ham does that thing have a needle attachment head...is that what the blunt tips are for? Damn a light bulb just went off!! All the times I've looked at them on sites it was never clearly described....now I see !!!
Inline pump assembly, correct.
Correct, the tube goes into the LC.
The tube is held in place by a media vessel-to-tube holder device.
Tube holder sold separately.
Had to shell out the extra cash for the tube holder though.
Would rather go with than without on the tube holder.
Otherwise, it was projected that tasking with gun would be even clumsier than already anticipated.
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Some call me Paw 🐾
When I get pulled over they ask for my autograph ✍️
WATUSI
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Fexus Boney Hadger
I care bigtime ®©™√
Registered: 12/23/09
Posts: 24,409
Loc: Spicemaster General.
Last seen: 1 hour, 30 minutes
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My stainless steel LC/I gun came in today.
The tube runs from the gun to the needle.
The needle goes into the media.
The gun has an inline pump action that pumps liquid into the glass barrel as liquid is aspired.
When the device is empty, it takes a few pump actions until the device and tube become full of solution.
The culture stock to test out gun is being generated (ATL#7's)
200, 2 foot tall, large gusseted spawn bags.
That'll last me a month.
Maybe two.
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Some call me Paw 🐾
When I get pulled over they ask for my autograph ✍️
WATUSI
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camplo
Freedom!
Registered: 02/10/08
Posts: 1,675
Last seen: 3 years, 4 months
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Re: Post Your New Tools Of The Trade. [Re: liquidclutter]
#21390782 - 03/10/15 11:48 PM (9 years, 10 months ago) |
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Quote:
liquidclutter said:
wut?
plastic syringes pc well many times
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Many times vs forever
The plunger leaks on disposables after several PC runs.
Glass syringe has glass plunger... Or atleast looks like it from the pic :/ mine will be here in the mail from overseas in a day or so. 12.99 shipped! order from the states same size, 50 bucks.
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 eatyualive
Eat's You Alive :)
Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Straya
Wot?!
Registered: 03/04/12
Posts: 705
Loc: 
Last seen: 5 years, 8 months
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Re: Post Your New Tools Of The Trade. [Re: eatyualive]
#21391470 - 03/11/15 06:25 AM (9 years, 10 months ago) |
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ItsScience
a dude
Registered: 12/12/12
Posts: 101
Last seen: 9 years, 1 month
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Re: Post Your New Tools Of The Trade. [Re: camplo]
#21394740 - 03/11/15 07:34 PM (9 years, 10 months ago) |
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Quote:
camplo said:
Quote:
liquidclutter said:
wut?
plastic syringes pc well many times
.
Many times vs forever
The plunger leaks on disposables after several PC runs.
Glass syringe has glass plunger... Or atleast looks like it from the pic :/ mine will be here in the mail from overseas in a day or so. 12.99 shipped! order from the states same size, 50 bucks.
Yeah, and it's shmexy.
duh
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-SCIENCE
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invitro
Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
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Re: Post Your New Tools Of The Trade. [Re: ItsScience]
#21394770 - 03/11/15 07:40 PM (9 years, 10 months ago) |
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I've never had a plunger leak, the glass ones, not having a rubber plunger have zero ability to push out contam spores that may be on the inside of the barrel during aspiration, and if you pull out the plunger too far, the airtight seal is broken, contam vector city. Also they are fragile at the plunger head, the glass is very thin there and not hard to break. Plastic is my pick.
And if your pc seal gives out half way through a cycle, you loose pressure fast, which can cause the glass to weaken and or crack.
Edited by invitro (03/11/15 08:06 PM)
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tourrat
humanbeinganimal
Registered: 01/16/14
Posts: 394
Last seen: 9 years, 4 months
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Re: Post Your New Tools Of The Trade. [Re: invitro]
#21394819 - 03/11/15 07:53 PM (9 years, 10 months ago) |
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Quote:
THE_LOAF said:
Quote:
Inline pump assembly, correct.
Correct, the tube goes into the LC.
The tube is held in place by a media vessel-to-tube holder device.
Tube holder sold separately.
Had to shell out the extra cash for the tube holder though.
Would rather go with than without on the tube holder.
Otherwise, it was projected that tasking with gun would be even clumsier than already anticipated.
Nice! Let us know how they work out! I'd like to see how much difference there is between the metal one and the plastic one .
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Knee deep in the hotel tub.....
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invitro
Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
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Re: Post Your New Tools Of The Trade. [Re: tourrat]
#21394836 - 03/11/15 07:56 PM (9 years, 10 months ago) |
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I'd think that putting the tube through a metal lid and siliconing it on would be way more reassuring for sterility... I'm assuming the tube is detachable from the gun.
Edited by invitro (03/11/15 07:56 PM)
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invitro
Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
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Monoject 140cc syringe
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Fexus Boney Hadger
I care bigtime ®©™√
Registered: 12/23/09
Posts: 24,409
Loc: Spicemaster General.
Last seen: 1 hour, 30 minutes
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Re: Post Your New Tools Of The Trade. [Re: invitro]
#21398059 - 03/12/15 02:48 PM (9 years, 10 months ago) |
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Quote:
invitro said:
I'd think that putting the tube through a metal lid and siliconing it on would be way more reassuring for sterility... I'm assuming the tube is detachable from the gun.
I agree
I am going to address this.
Give me a little while.
I have a system figured out I'd like to share with you.
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Some call me Paw 🐾
When I get pulled over they ask for my autograph ✍️
WATUSI
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insanemike
Registered: 02/23/14
Posts: 4,272
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Quote:
THE_LOAF said:
Quote:
invitro said:
I'd think that putting the tube through a metal lid and siliconing it on would be way more reassuring for sterility... I'm assuming the tube is detachable from the gun.
I agree
I am going to address this.
Give me a little while.
I have a system figured out I'd like to share with you.
 can't wait.
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tourrat
humanbeinganimal
Registered: 01/16/14
Posts: 394
Last seen: 9 years, 4 months
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Re: Post Your New Tools Of The Trade. [Re: insanemike]
#21398729 - 03/12/15 05:00 PM (9 years, 10 months ago) |
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Yeah I'd also like to know if that gun would work with a brf cake slurry if it was watered down some...that would be an amazing system!
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Knee deep in the hotel tub.....
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invitro
Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
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When making your design, don't make it so the tube hangs into the LC during colonization. The lc will grow in the tube and clog it. The gun will not have enough suction power to overcome the lc-plug in the tube, and even if it could, it would clog the syringe.
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Fexus Boney Hadger
I care bigtime ®©™√
Registered: 12/23/09
Posts: 24,409
Loc: Spicemaster General.
Last seen: 1 hour, 30 minutes
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Re: Post Your New Tools Of The Trade. [Re: invitro]
#21400243 - 03/12/15 10:57 PM (9 years, 10 months ago) |
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Here's a quick look at what I figured out.
Design was inspired by the example photo in the instruction booklet.
The same design can be replicated with metal lids and silicone as you described above.
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Some call me Paw 🐾
When I get pulled over they ask for my autograph ✍️
WATUSI
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invitro
Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
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I go to the absolute extreme when it comes to sterility on LC so keep that in mind with the following comment:
Those plastic lids can introduce foreign spores by means of the space between the lid and the glass rim, unless you have a decent gasket. That lid will probably work 99%, but I've seen them fail in just the way I described. To put it another way, air currents can cross the barrier between the lid and the glass rim no matter how tight the lid is cranked down.
Edited by invitro (03/12/15 11:10 PM)
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Fexus Boney Hadger
I care bigtime ®©™√
Registered: 12/23/09
Posts: 24,409
Loc: Spicemaster General.
Last seen: 1 hour, 30 minutes
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Re: Post Your New Tools Of The Trade. [Re: invitro]
#21400326 - 03/12/15 11:19 PM (9 years, 10 months ago) |
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No gaskets are used.
Instead parafilm is triple wrapped around the space between jar lid and glass.
Mediums are left inside the vacuum of the AA until inoculation.
LC lids are removed for inoculation in front of a flowhood in an enclosed lab.
Then the parafilm is wrapped onto the media vessel.
Directly in the downstream of the laminar flowhood.
The mediums are then placed on a shelf and incubated at 70-75F & 40-55RH.
Contams travel in air currents.
Air currents will take the path of least resistance.
These principles are crucial as to why it's critical that a GE filter be placed on an LC media vessel closure.
So that the path of least resistance for air to enter your media is through a media filtration device.
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Some call me Paw 🐾
When I get pulled over they ask for my autograph ✍️
WATUSI
Edited by liquidmyce (03/12/15 11:46 PM)
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