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 ManifoldPrime
Per Ardua Ad Astra
Registered: 03/16/17
Posts: 1,313
Loc: South Africa 
Last seen: 1 year, 3 months
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 Re: c10's Agar Guide + Tips & Tricks (post #1000)  [Re: Psychedeliciouss]
#24836907 - 12/09/17 11:53 PM (6 years, 4 months ago) |
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If you wrap them with parafilm and store them in something airtight, E.G a ziplock bag or a tupperware, should be fine. Would also show if your technique isnt good considering any plates that would contam due to tek would do so over a month.
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My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
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 Mateja
 
Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: ManifoldPrime]
#24836909 - 12/09/17 11:59 PM (6 years, 4 months ago) |
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Quote:
ManifoldPrime said:
Would also show if your technique isnt good considering any plates that would contam due to tek would do so over a month.
Hes talking about storing cultures in the fridge
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Cakes inside Water Tub
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Psychedeliciouss
Scrappy CoCo
Registered: 10/18/17
Posts: 110
Last seen: 5 years, 4 months
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: ManifoldPrime]
#24836986 - 12/10/17 02:24 AM (6 years, 4 months ago) |
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thank you 
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Snickelfritz
Registered: 11/24/17
Posts: 2
Last seen: 6 years, 1 month
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 Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Psychedeliciouss]
#24889755 - 01/04/18 01:00 PM (6 years, 3 months ago) |
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Thanks for this!
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Fungeyes
Stranger
Registered: 08/26/17
Posts: 136
Last seen: 2 years, 7 months
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Snickelfritz]
#24895955 - 01/07/18 09:02 AM (6 years, 3 months ago) |
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Great read booked for later studies
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Pablo1211
Stranger
Registered: 01/14/18
Posts: 1
Last seen: 6 years, 2 months
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: mushboy]
#24913146 - 01/14/18 10:26 AM (6 years, 3 months ago) |
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This is awesome! Thx for the details
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2Dot
Registered: 01/23/18
Posts: 45
Loc: Half Step Away
Last seen: 3 months, 26 days
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Pablo1211]
#24941070 - 01/25/18 01:08 PM (6 years, 2 months ago) |
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Gonna be getting into agar, and I especially liked the streaking technique, gonna use that, Thanks for the experienced info.
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w33dh4x
Stranger
Registered: 11/20/17
Posts: 26
Last seen: 6 years, 29 days
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
#24950258 - 01/28/18 10:48 PM (6 years, 2 months ago) |
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Thank you for this. First time grower here and decided to start with spores on agar. This has been my bible and hasn't failed me yet.
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R.I.P.Zappa
Myco Melyco
Registered: 03/30/18
Posts: 1,212
Loc: In Between Space and Time 
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: w33dh4x]
#25116373 - 04/05/18 04:57 PM (6 years, 12 days ago) |
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c10h12n2o, I think I just found my hero.
Started a couple grain bags and some BRF jars a week ago. To keep me occupied while they colonize, I decided to jump straight into agar for hands on experience and put some use to my left over syringe.
Learned a few things right off the bat; My 64q SAB is to short. My petri stand to keep space between the cloth on the bottom and my petri dishes is too high along with my 1000ml slimline media bottle.
Gonna have to make some adjustments, thinking a home made plexi glass one may be the way too go. Next step, knock one up and see how my other dishes grade my sterile technique. "Like a blind man in an orgy ya gotta feel things out", I guess.
Great write up, fuel to my newfound fire, thank you for sharing!
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-The heaviest thing one will ever carry is a thought-
-"Like a Blind man In an orgy you gotta feel things out.".-
-When we agree about our hallucinations, we call it “reality".-
-If you defy authority because your told to, that's no better than blindly trusting authority.-
psychonautwiki.org
How it should & shouldn't look - NEW CULTIVATORS GUIDE
BOD's Easy AF OAT prep tek.
Principles of mushroom growing for beginners
Edited by R.I.P.Zappa (04/05/18 04:59 PM)
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bison2
Stranger
Registered: 03/26/17
Posts: 35
Last seen: 6 months, 22 days
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
#25149190 - 04/18/18 07:37 PM (5 years, 11 months ago) |
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C10,
First of all let me praise you for the clear and concise TEK you’ve provided and let you know that I realize, how much time and effort you’ve put into doing it. Just amazing!
Now, first an excuse: I’m (as of this coming June) 80 years old. I’ve just started cultivating psilocybe cubensis in the last few months and I’m having a difficult time navigating the Shroomery board and absorbing all the information available.
I’ve spent the last several hours reading your TEK and a number of others. At this point I seem to be in overwhelm I’ve finished PCing a few agar dishes using Gladware plastic mini-containers and I have a spore print ready to use.
Here’s my question: What’s the best method of getting the spores from the spore print onto the agar? I don’t seem to find in your TEK how you’re transferring the spores onto the agar. It’s probably just me. I’ve started to slow down in my comprehension these last couple of years. Are you just using an inoculating loop to scrape some spores from the print?
There’s no need to write something out and waste your time. If you could just point me to the right post. I’ve tried searching and just haven’t been able to come up with a clear procedure.
I noticed that somewhere in your description you mention not to drop any liquid from a syringe onto the petri dish as that just leads to contamination and spreading the mycelium growth indiscriminately across the agar.
Any advice or suggestions you might provide would be greatly appreciated.
Thanks in advance,
JB
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R.I.P.Zappa
Myco Melyco
Registered: 03/30/18
Posts: 1,212
Loc: In Between Space and Time
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: bison2] 1
#25149503 - 04/18/18 09:06 PM (5 years, 11 months ago) |
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These may help. Gotta do allot of digging to fill in the blanks. Sometimes ya find the answer to what you are looking for in a thread not even related to what you are looking for. Good thing is you never run out of stuff to read and often find somthing new.
Here is a good one for how to use the search engine better.
https://www.shroomery.org/forums/showflat.php/Number/24270830#24270830
How to use the sites features
https://www.shroomery.org/forums/showflat.php/Number/23745550
Heres is a bunch of compiled agar thread links.
https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954
Simple example:
I copy pasted your, "spore print onto the agar", from your sentence,
"Here’s my question: What’s the best method of getting the spores from the spore print onto the agar?", onto the search bar on the top and came up with this.
https://www.shroomery.org/forums/showflat.php/Number/20296483#20296483
Could also ask in these two threads as well.
https://www.shroomery.org/forums/showflat.php?Cat=&Board=83&Number=25145624&fpart=1&PHPSESSID=
https://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=24679216&fpart=1&PHPSESSID=
There is more than one way to skin a cat and there is allot of info and helpful folks here.
Welcome and 
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-The heaviest thing one will ever carry is a thought-
-"Like a Blind man In an orgy you gotta feel things out.".-
-When we agree about our hallucinations, we call it “reality".-
-If you defy authority because your told to, that's no better than blindly trusting authority.-
psychonautwiki.org
How it should & shouldn't look - NEW CULTIVATORS GUIDE
BOD's Easy AF OAT prep tek.
Principles of mushroom growing for beginners
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k5hd2y
Depersonalized & Complete
Registered: 04/13/17
Posts: 675
Last seen: 4 years, 5 months
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: R.I.P.Zappa]
#25255177 - 06/07/18 10:49 PM (5 years, 10 months ago) |
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Nice! just chiming in to track this thread. 
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verum subsequentis
seeker of truth
Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 10 months
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: k5hd2y]
#25255235 - 06/07/18 11:57 PM (5 years, 10 months ago) |
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this thread is dead for the most part. c10 hasn't been around for a while. it's a good right up though
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Casi Loco
Registered: 08/31/18
Posts: 6
Last seen: 5 years, 5 months
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
#25465191 - 09/16/18 05:29 PM (5 years, 6 months ago) |
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Very detailed,,thanks for taking the time to share it.
Happy I found this post, will bookmark it for sure.
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! When the system you live in has destroyed all the fun, the only fun left is to destroy the system !
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Scrubbs
Stranger
Registered: 09/16/18
Posts: 10
Last seen: 5 years, 6 months
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Casi Loco]
#25466229 - 09/17/18 02:26 AM (5 years, 6 months ago) |
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Super helpful write up c10h12n2o, great to see so many pix
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Nowheremans
Registered: 10/06/18
Posts: 255
Loc: on the spiral
Last seen: 1 year, 4 months
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Scrubbs]
#25534235 - 10/13/18 10:28 AM (5 years, 6 months ago) |
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thanks for sharing! Great wealth of information there
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Casi Loco
Registered: 08/31/18
Posts: 6
Last seen: 5 years, 5 months
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
#25565207 - 10/25/18 01:37 AM (5 years, 5 months ago) |
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How can I utilize the agar dishes to inoculate PF cakes ?
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! When the system you live in has destroyed all the fun, the only fun left is to destroy the system !
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verum subsequentis
seeker of truth
Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 10 months
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Casi Loco]
#25566225 - 10/25/18 11:27 AM (5 years, 5 months ago) |
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this thread is DEAD my friend. agar to brf cake is not a normal way of doing this but you could (plausbly) just put it on top with no verm layer. better yet would be using clean agar to make a clean lc and then noccing up the cake with the lc. or... make an LI out of the agar and use that. bottom line though is brf cakes are for beginners who are NOT using agar. there is nothing wrong with still using brf cakes but grain spawned to bulk will give you better yields. brf cakes are for people who are still just using spores. spore syringes aren't all to clean and brf cakes are really good at mitigation bacterial infection.
... get yourself some wild bird seed (or other grain) and noc that shit up proper. just my two cents.
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bodhisatta  
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Casi Loco]
#25566682 - 10/25/18 02:39 PM (5 years, 5 months ago) |
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Quote:
Casi Loco said:
How can I utilize the agar dishes to inoculate PF cakes ?
Make a cake with a grain jar lid (has filtered gas exchange), skip the dry verm barrier since your lid is now the filter. Then inoculate it like a grain jar.
Now for me to sound mean but if you have to ask i would stick to the beginner methods, get feet wet. And when all these terms and techniques make sense and are not so overwhelming and confusing then its time to branch to "advanced" methods.
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stareatclouds
star eat clouds?
Registered: 09/29/14
Posts: 10,128
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: bodhisatta]
#25567006 - 10/25/18 05:08 PM (5 years, 5 months ago) |
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Or fuck it, do everything at once. It can work. 
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