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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Post Your New Tools Of The Trade. [Re: camplo]
    #21460917 -

Hell yea man! I just got my first AA... I think this thing will last 1000 years

Kinda wish I checked craigslist first :lol:


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Amateur Mycologists United
AMU Q&A

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Re: Post Your New Tools Of The Trade. [Re: TheChief]
    #21462245 -

TheChief said:
They PC just fine IME, punx!  And no rust! :stoner:


:chief::rockon:

chief!


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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Re: Post Your New Tools Of The Trade. [Re: eatyualive]
    #21462646 -

Howdy, Eat!

Did you see that new PC I just bought?  Thing is huge!


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jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."

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Re: Post Your New Tools Of The Trade. [Re: TheChief]
    #21463568 -

nice!

for the lids, im not sure what brand mine were but they were a pressure cooker variety obtained at the local grocery store. and they work great without any warping. with sfd and ships for lc's!:chief:



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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

Edited by eatyualive (03/26/15 11:19 PM)

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Re: Post Your New Tools Of The Trade. [Re: eatyualive]
    #21463643 -

Well, all my cheap, grocery store bought plastic lids have warped on me (ball and mainstays brands).  Not to the point to where I am getting contams as a result.  More so that they are a huge PITA to get loosened up after they go through the sterilizer.

My alcohol soaked gloved hands have all to often slipped off of the closures and into the flowhood's HEPA more times than I want to talk about.  Can't be having no slip action into the HEPA when I build the new flowhood.  These new lids have ridges in a tight pattern all the way around the lid for better gripping-action, as well.

I sterilize at slightly higher than average psi's though, and is most likely the reason cheap lids warp on me.  The ones myco/agar/mycota/6Tango sent me haven't warped at all out of all the five years I have owned them.  That tells me something loud and clear.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Post Your New Tools Of The Trade. [Re: hamloaf]
    #21465081 -

Well the autoclave lids Ive seen for pretty fair price around from various cultivation supply sites, box of 100 close to 30 bux, more expensive than the grocery store type but fair enough I say since Im going qt-spawn bag I doubt Ill be using more than 100 grain masters at any one time in my small house :lol:

Can dream though!


I tottally get you Loaf, oh man trying to loosen lids with ISO slathered gloves? Ugh Ive smacked into the hood more times than I like to admit.
My Hepa is in great shape regardless, youd never know it because I have an Awl that works pretty well at rounding out the pins.

Of course a real hard smack and I dont think Id be able to undo that damage haha
I wonder, is there some sort of protective cage you put on that wouldnt disrupt flow?

Loaf- cant wait to see you build that big boy man ohhh its gunna be sweet !


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Amateur Mycologists United
AMU Q&A

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Re: Post Your New Tools Of The Trade. [Re: mushpunx]
    #21465166 -

I always just rub my hands together until the iso alcohol is pretty much dried away...  Never have any problems with contam.  I don't even use it that much.  :shrug:

I think there is a lot more paranoia around being clenly and getting contams than there needs be...  I'm actually quite sloppy sometimes when it comes to myself being clean; but, I find as long as your movement tequniques in the SAB/flowhood are fairly good and your media/spawn is properly sterilized/clean contams will rarely come about.  Unless you just wiped your arse after taking a dump and don't wear gloves at all, you should be fine.  IME, I find no need to be overkill with sterility.  :shrug:

Edited by TheChief (03/29/15 08:38 AM)

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Re: Post Your New Tools Of The Trade. [Re: TheChief]
    #21465378 -


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Re: Post Your New Tools Of The Trade. [Re: blackdust] * 1
    #21467632 -

just finished some like it, did 28 and ran out of sdf dammit
fresh and clean :awesomenod:


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Re: Post Your New Tools Of The Trade. [Re: cArcace-x]
    #21482616 -

12 - 70mm (regular mouth) rubber lid gaskets from ShroomSupply, and 20 - 6" threaded, 20mm x 150mm, glass culture tubes with caps from DiscountVials.



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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Post Your New Tools Of The Trade. [Re: hamloaf]
    #21482784 -

are the seal in the vial cap are ok for slant storage?
i have similar to that and was wondering


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Re: Post Your New Tools Of The Trade. [Re: cArcace-x]
    #21483132 -

The caps to the vials seal like a champ.  :thumbup:

Be sure to loosen the caps for sterilization.  Once inoculated, I like to wrap the seal where the cap meets the threads with parafilm for added security.



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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Post Your New Tools Of The Trade. [Re: hamloaf]
    #21492995 -

100 BD 10ml vacationer tubes with super heavy duty red self healing injection ports as cap + 10 - 90mm (large mouth) rubber gaskets.



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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Post Your New Tools Of The Trade. [Re: hamloaf]
    #21493040 -

Why do you need the ship's on that? You planning on using them as slants or what?


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Dream it, plan it, build it, do it

Every year is getting shorter, never seem to find the time...

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Re: Post Your New Tools Of The Trade. [Re: Big Bear]
    #21493180 -

Gonna try them out as slants, yes.  If they don't work out, then back up slants are had.  Also, if these don't work out as slants for me, then I got a great deal on 100 - 1/2 inch, red injection ports.  Those red SHIP's run 85 - 99 cents a piece when purchased on their own.  Each sold separately.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Post Your New Tools Of The Trade. [Re: hamloaf] * 1
    #21493622 -

spawn bags with filter patches work so damn well.



even for invitro pans.



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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Re: Post Your New Tools Of The Trade. [Re: eatyualive]
    #21493625 -

i also love me some vacutainers. great method for storage.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Re: Post Your New Tools Of The Trade. [Re: eatyualive]
    #21493929 -

ham, I found an awesome deal on amazon for 50ml plastic tubes I'm using for slants now.
non tapered opening makes going in an out really easy w/o bumping the walls of the slant with stem of the tool, which may or may not have been flame sterilized red hot

increased volume enables more pulls from each slant, in addition to longer storage potential

and they also have "skirts" so u can stand them upright w/o a rack if necessary.

i was using 25 ml glass tubes previously
its $11/50 50ml tubes, caps included.

here
currently unavailable...but they usually come back in a month or so.


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Re: Post Your New Tools Of The Trade. [Re: blindingleaf]
    #21493937 -

i was thinking of trying out those vacutainers for one time use LC/LI kinda thing.
one tube>1-3 qt masters>bags
no need to store plates.  pour and colonize.  less risk for contamination was my main idea, what do u think?


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Re: Post Your New Tools Of The Trade. [Re: blindingleaf]
    #21494425 -

blindingleaf said:
i was thinking of trying out those vacutainers for one time use LC/LI kinda thing.
one tube>1-3 qt masters>bags
no need to store plates.  pour and colonize.  less risk for contamination was my main idea, what do u think?


ive stored agar cultures in liquid form and even tissue in those. they work great. you can later use a sterile syringe to suck them back up for a quick inoculation.
i recently put a liquid culture oyster syringe on agar from 2007. it colonized in 3 days from one drop of the syringe. not too bad for 8 years ago.

pearl and blue. i am making more plates to transfer as we speak. in addition, i inoculated a bunch of prints from 2003 that all germinated. awaiting a transfer now!


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Shop: Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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