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Invisibleno-tone
Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
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PCing tools of the trade
    #1445759 - 04/10/03 07:01 AM (13 years, 7 months ago)

A friend PCed some of his syringes, vials (for mycelial water), syringes, tweezers, and inoculation loop yesterday. He was wondering if he used a technique that would suffice to sterilize the equpiment.

7 used syringes: sucked bleach into chamber 4 times, sucked water into chamber 4 times. Sucked water up one last time. Wrapped in foil, in the PC.

Loop, tweezers, syringe: wrapped in foil, tossed in.

Vials: 20 ml vials with holes drilled in lid. Broken glass to chop up mycelia inside. Foil layer under cap. Whole thing wrapped in foil, tossed in PC.

Everything PC'd @15psi for 70 mins. Should everything be ok for him to use now?


--------------------
Man thinks. God laughs. - Jewish Proverb


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InvisibleRoadkill
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Registered: 12/11/01
Posts: 22,598
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Re: PCing tools of the trade [Re: no-tone]
    #1445762 - 04/10/03 07:06 AM (13 years, 7 months ago)

Yes

sounds good.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineoO_wombat_Oo
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Re: PCing tools of the trade [Re: no-tone]
    #1445764 - 04/10/03 07:07 AM (13 years, 7 months ago)

Yes he did.

My friend (that's actually me, BTW) doesn't bother with any of that shit and hardly anything gets contaminated.

My friends (again, that's really me) gives his "tools of the trade" a fry in the microwave or a quick boil.

Nazi like sterilisation is part-and-parcel with mushroom cultivation, but I've never had any serious, unexpected problems with contams. It's all just common sense. Don't breath on shit. Keep shit clean.

Personally, I think you went way over the top, but shit-eh. It's not gonna hurt! :wink:


Edited by oO_wombat_Oo (04/10/03 07:09 AM)


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Invisibleno-tone
Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
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Re: PCing tools of the trade [Re: oO_wombat_Oo]
    #1445778 - 04/10/03 07:21 AM (13 years, 7 months ago)

Great, thanks to both of you!


--------------------
Man thinks. God laughs. - Jewish Proverb


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Offlinedebianlinux
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Re: PCing tools of the trade [Re: no-tone]
    #1445857 - 04/10/03 08:54 AM (13 years, 7 months ago)

2 things. 1) unless your sterilizing grains 70 minutes is a bit long, i'm thinking more like 15-20. 2) Bleach is tenacious (tho PCing will prolly break it's bond) and I would suggest you use alcohol when cleaaing the syringes.


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Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
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Re: PCing tools of the trade [Re: debianlinux]
    #1445871 - 04/10/03 09:07 AM (13 years, 7 months ago)

He was sterilizing 2 WBS qt jars in a 22 qt PC. Needless to say, he had some extra room and wanted to use it.

He'll use alcohol next time. Do you think such little residue will harm the inoculant?


--------------------
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OfflineoO_wombat_Oo
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Re: PCing tools of the trade [Re: no-tone]
    #1447913 - 04/10/03 09:42 PM (13 years, 7 months ago)

If you use those alcohol wipes, the alcohol evaporates into the air really quickly.


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Amazon Shop for: ½ Pint Jars, Wild Bird Seed

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