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OfflineGr0wer
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anyone ever try sterilizing a syringe without PCing?
    #3187208 - 09/27/04 06:18 PM (12 years, 2 months ago)

Has anyone ever sterilized a syringe with alcohol + h2o2?

I was thinking this, fill a clean syringe with alcohol let it sit for 1 min this should kill everything. Then empty and fill with 1.5% H202 this should get rid of the nasty alcohol. Now dump the h2o2 when your ready and fill with karo water. Would this work well?


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OfflineKillBill
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
    #3187878 - 09/27/04 08:45 PM (12 years, 2 months ago)

Interesting that you should ask that, the other day i realized i would need a new sterile syringe(s), and didn't feel like pressure cooking them, so i sucked up 91% Isopropyl alcohol , spat it out, then sucked up H202 shook vigorously and spat it out. I then used liquid Inocculant on some rye jars. its been two days, a few jars so activity but here are some things that weirded me out. 1. my liquid myc has been in a fridge for a long time, maybe a month or longer and its gonna be 40 degrees in there, they did not Freeze but i feel like perhaps It did some damage to it since a lot of the jars aren't showing activity. Also when i had hte liquid inocculate in the syinge, i noticed a lot of tiny bubbles all over the place. I assume this was from H202 oxidizing(?) some of the myc but it definitely weirded me out.

More to the point though, while growth is limited in the jars i did, there are no contams present, suggesting that alcohol followed by h202 is a sufficient means of sterilizing, saving us time and gas money.

:-D Damn i feel smart, having helped someone who knows infinitely more than I.


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Offlinehyphae
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
    #3188137 - 09/27/04 09:40 PM (12 years, 2 months ago)

Yep microwaved water. Draw up and purge water (right out of the microwave) several times works great!


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OfflinePsilocybeingzz
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
    #3188188 - 09/27/04 09:49 PM (12 years, 2 months ago)

I use boiled water, a'la Psilocybers syringe TEK, from the shroomery archives. :wink:


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Edited by Psilocybeingzz (09/27/04 09:50 PM)


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OfflineCornelius
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
    #3188252 - 09/27/04 10:03 PM (12 years, 2 months ago)

yeah, I boil the water for about 10 minutes then suck and purge a few times as per the syringe tek...


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Invisiblegeorge castanzaM
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
    #3188262 - 09/27/04 10:04 PM (12 years, 2 months ago)

kramer says he has not so much as tried it. :shocked:
as lazy as he is he likes the idea though! :crazy2:
:sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :sun: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :tongue2:


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Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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Offlinehyphae
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
    #3188334 - 09/27/04 10:16 PM (12 years, 2 months ago)

The microwave gets that water so molecularly hot much hotter than boiling water could ever get, this is what makes this tek work 100% of the time. FYI guys be very careful!


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Offlinemockeylock
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Re: anyone ever try sterilizing a syringe without PCing? [Re: Gr0wer]
    #3188338 - 09/27/04 10:17 PM (12 years, 2 months ago)

It doesn't get much easier than boiling water...and it works well.

I'd keep the chemicals out of my syringes, but that's just me.


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OfflineGr0wer
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Re: anyone ever try sterilizing a syringe without PCing? [Re: mockeylock]
    #3188899 - 09/28/04 12:08 AM (12 years, 2 months ago)

thanks!


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Offlineatomic1
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Re: anyone ever try sterilizing a syringe without PCing? [Re: KillBill]
    #3189437 - 09/28/04 01:34 AM (12 years, 2 months ago)

Thats too weird you did that. Last wed. or thu. i did the exact same thing. EXACT. Rubbin alcohol 1st, i let it sit for a couple min.'s, then followed by drawing up h202. I then used some karo liquid that had been in the fridge for a few months actually several months. Anyway today, monday, i'm still yet to see ANY growth, mycelia or mold. I don't know what happend. I used some of this karo a few weeks ago and it worked fine. Now its nothing. If I was to have not sufficiently got out all the alcohol would it have killed my karo mycelia? I'm stumped. Any help would be great.


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OfflineGr0wer
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Re: anyone ever try sterilizing a syringe without PCing? [Re: atomic1]
    #3192275 - 09/28/04 06:56 PM (12 years, 2 months ago)

Just checking but do you guys still boil the syrines or just suck in/out superheated sterile water?


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Amazon Shop for: ½ Pint Jars, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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