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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Stro's Cleaning and Isolating On Agar [Re: rudimentary]
    #26747078 -

Curious if anyone knows how to get myc on agar to form rhizomorphic growth.  I'm well aware that it can always go back and forth, and that there are great strains that are tomentose, but for my purposes I often find that rhizomorphic allows me to identify sectors easier more often than tomentose.  Wanted to see if anyone has any input on the matter.  I find that if I take an isolate or mycelium with multiple genetics that is on a piece of grain, and transfer the grain to the agar, I often (roughly 90% of time) will get rhizomorphic growth.  If I go from agar to agar, it seems tomentose around 80% and 20% rhizo.  Anyone think perhaps more complex food sources have something to do with this?  Most of my agar preps are PDA or MEA - I've tried fiddling around with nutrient density in those dishes, but doesn't seem to have much effect for me.

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Re: Stro's Cleaning and Isolating On Agar [Re: Lu-Kthu]
    #26747388 -

For me thinly poured plates with like 4g lme to 400-500ml does it most of the time.


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LAGM2020

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Re: Stro's Cleaning and Isolating On Agar [Re: A.k.a]
    #26747467 -

That is super thin. Like SSLI thin if your doing 4 g of LME.

I usually do 6-7 LME 10agar.

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Re: Stro's Cleaning and Isolating On Agar [Re: A.k.a]
    #26748826 -

Iv never had tomentose growth on agar only rizomorphic I clone from fruits around the base of the stem I get rizomorphic off the first dish an easy can see sectors

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #26758383 -

Thank you for writing this. Iā€™m new to agar, and am really appreciative of you writing this.

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #26846278 -

Very handy write up, appreciate you sharing with us. :thumbup:

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Re: Stro's Cleaning and Isolating On Agar [Re: bernardfraun]
    #26846658 -

.


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #26938744 -

Awesome man, thanks!

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Re: Stro's Cleaning and Isolating On Agar [Re: Profbri]
    #26951257 -

Thank you for the knowledge, I'm just starting and you answered a ton of questions!

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Re: Stro's Cleaning and Isolating On Agar [Re: Onset11]
    #26988830 -

10/10 newb stars! :smile: Thanks


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Knowledge and Healing in a Handful.

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #27051712 -

Nice write up Sir.. These will surely prevent any newbie from committing mistakes.

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Re: Stro's Cleaning and Isolating On Agar [Re: SilverSloth]
    #27058982 -

bookmarking

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Re: Stro's Cleaning and Isolating On Agar [Re: bunsie]
    #27097304 -

Great write up! Bookmarked.

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #27164826 -

thank you for the write-up Stro!  this helped me a ton in learning how to see the sectors and where to transfer from, among a bunch of other little details.  nice and simply explained. again, thank you!

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #27347419 -

brilliant, one of the best threads here.

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #27537828 -

Thank you for this Stro! 

I read about 'strain isolation' on agar in a bunch of places
but the process only became really clear after your post.

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #27606337 -

what a great post.
I plan to do this with a spore syringe since my first mono tub was a fail.
My question is how long can you keep an agar plate thats inoculated  before placing in say a spawn bag.  Also when do you know it is ready to go into a spawn bag.
Also can you keep dish C for a while stored in the fridge or dark space Before using to inoculate.

I plan to buy some pre-made agar dishes on amazon since I don't have a PC and for a first time seems cheaper to get them rather than all the stuff I would need plus time.

If there is a good write up on next step l would love to see it. I know there is a lot of good stuff on here just overwhelming sometimes and I seem to go down too many rabbit holes ha!

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Re: Stro's Cleaning and Isolating On Agar [Re: rotor7]
    #27621553 -

This is the perfect time for me to stumble on this write up! Good shit! Plenty of questions I had answered! Thanks for this!


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LAGM V2.022! GROW LOG!
https://www.shroomery.org/forums/showflat.php/Number/27601367#27601367

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #27683988 -

Quote:
They were however moved to a higher temperature for colonization.  There appearances speak for themselves. The colonization times were faster and characteristics are distinctive. If I don't keep medium and conditions consistent, the mycelium tricks me up.


tnx for great info, well done. but here I don't get this. how mycelium trick you? you mean if temperature is not consistent the pattern would be different and they can appear as sector or contamination?

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Re: Stro's Cleaning and Isolating On Agar [Re: puya]
    #27691548 -

Thanks for this! Very informative! Ive been struggling with figuring out what to transfer in order to clean up my sample, this definitely gave me an "ah-ha" moment.

:cheers:


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Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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