bodhisatta said:

denger said:

bodhisatta said:

No that would be the third way. By the time you see mycelium on a dish its long been dikaryotic if you streaked out spores

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That depends on the density of the streak. If it shows single colonies with some distance between them they could be monos with some probability.

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I purposely diluted spores down quite a bit then streaked them. Looked at with a microscope there's still just too many spores. And spores like to clump. If you could dilute spores and also keep them from grabbing a partner then maybe. But even using jet dry or other surface tension agents wasn't able to get spores broken apart enough to get monokaryotic growth no matter how hard I tried. I think it would take a micromanipulator scope to achieve single spore monokaryotic growth reliably

Even with hefty agitation you'll see clumps like this


Diluted and streaked


If you made a 10cc syringe with the smallest speck of spores you can see its still too many spores


Even at the hardly visible growth phase they're long dikaryotic from streak plates(not super diluted in this case)



Ive played around a bunch with germination and trying to get monokaryotic growth but i think its out of the reach of hobby equipment


Spores clump like madmen despite your best efforts to make them disperse evenly

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cronicr said:

You cut a tiny little wedge

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