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OfflineTheHobbit
Pot Head Pixie

Registered: 09/04/02
Posts: 863
Loc: the Oily Way...
Last seen: 17 years, 11 months
Why go to the trouble of the agar approch anyway?
    #1019137 - 11/03/02 09:02 PM (18 years, 10 months ago)

Just kind of curious, given the relative ease of syringes.....just newbie curiosity. :confused:


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OfflineStS
treetoker
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Registered: 08/01/00
Posts: 1,821
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Re: Why go to the trouble of the agar approch anyw [Re: TheHobbit]
    #1019145 - 11/03/02 09:06 PM (18 years, 10 months ago)

Growing on agar helps to isolate the strongest mycellium from a large spore batch. In the end it helps keep the genetics of a strain alive and healthy.


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"You took too much, man....too much, too much!"


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OfflineEnoch
prophet
Registered: 10/17/02
Posts: 15
Loc: Illinois
Last seen: 18 years, 10 months
Re: Why go to the trouble of the agar approch anyway? [Re: TheHobbit]
    #1019177 - 11/03/02 09:22 PM (18 years, 10 months ago)

agar is the way any strain is isolated. mushrooms began in nature, thus cultivated mushroom strains were isolated from wild mushroom spores


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InvisibleOlgualion
Shaman-In-Training
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Registered: 11/14/01
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Re: Why go to the trouble of the agar approch anyway? [Re: TheHobbit]
    #1019701 - 11/04/02 12:57 AM (18 years, 10 months ago)

There are two different approaches to strain isolation on agar. First, is the approach where you innoculate multispore onto agar, then sector out the best growth, which hopefully will be a nice strain.

IMO - the other approach is better. Go multispore to grain, let the strains fight eachother off, and IMO that strain who really wants to be kept will make its way to fruit. You will see it, it will say clone me, then you have your great strain. Juat an added bit of info is - the BEST isolate I've ever seen was NOT rhizomorphic!


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Study the past...
See the future...


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Why go to the trouble of the agar approch anyway? [Re: TheHobbit]
    #1019737 - 11/04/02 01:12 AM (18 years, 10 months ago)

Look at it this way.

A multispore syringe is like a bitch dog, that got nailed all week by every horny male dog around for 2 miles.

The resulting pups, could range from tiny cross eye mongels to retarded muts, or a mix & match of all donors. Alternativly, with a selectivly bred dog -- you know what the pups are going to be like.

An isolate or clone strain is simply selective breeding. Selective breeding gives more consistant & better results. Simple as that.

6T  :tongue: 


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~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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OfflineHisStudent
enthusiast

Registered: 02/01/02
Posts: 206
Last seen: 12 years, 9 months
Re: Why go to the trouble of the agar approch anyway? [Re: SixTango]
    #1019776 - 11/04/02 01:33 AM (18 years, 10 months ago)

Nice analogy 6T.

I know i've fruited my fair share of cross-eyed retard mongrel mutts. :tongue:
 


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I don't think I'm alone when I say I'd like to see more and more planets fall under the ruthless domination of our solar system.
- Jack Handey


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InvisibleZen Peddler
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Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: Why go to the trouble of the agar approch anyway? [Re: TheHobbit]
    #1020246 - 11/04/02 08:51 AM (18 years, 10 months ago)

Multi spore is like pot luck - sometimes you get a good strong genetic spore match, sometimes otherwise - with agar cloning or isolation you are evening the odds for a good spore match.
With isolation however you have to make the assumption that the rhizomorphic mycelia is going to perform well fruit-wise.
With cloning although you may be cloning a strong fruit, the result will not necessarily guarantee lots of fruits of the same vigour


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Offlineblackout
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Re: Why go to the trouble of the agar approch anyway? [Re: Olgualion]
    #1020535 - 11/04/02 11:39 AM (18 years, 10 months ago)

"IMO - the other approach is better. Go multispore to grain, let the strains fight eachother off, and IMO that strain who really wants to be kept will make its way to fruit. You will see it, it will say clone me, then you have your great strain. Juat an added bit of info is - the BEST isolate I've ever seen was NOT rhizomorphic! "

also that strain growing on the rye is a strain which obviously likes rye. i.e. if you were planning on growing on millet then start with millet


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OfflineTheHobbit
Pot Head Pixie

Registered: 09/04/02
Posts: 863
Loc: the Oily Way...
Last seen: 17 years, 11 months
Re: Why go to the trouble of the agar approch anyway? [Re: blackout]
    #1020541 - 11/04/02 11:44 AM (18 years, 10 months ago)

Well, those answers clear that up for me. Maybe I'll get into that after I've gotten my feet notably wetter. Thanks guys!


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OfflineShr00mmz
Stranger

Registered: 06/24/02
Posts: 20
Loc: << That Way >>
Last seen: 18 years, 6 months
Re: Why go to the trouble of the agar approch anyway? [Re: HisStudent]
    #1041748 - 11/10/02 09:58 PM (18 years, 10 months ago)

I know this is an old post, but I couldn't stop laughing at the "fruiting my share of cross-eyed retard mongrel mutt" comment. Too funny!!


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