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Invisibleohmatic
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rhizomorphic isolate going back to linear / tomentose
    #3647087 - 01/19/05 05:14 AM (11 years, 10 months ago)

ey!

i isolated a rhizo sector from a plate on 2 different plates,
but instead of growing the selected characteristiks they have both
begun to grow normal linear mycelium in radial fine strands.

i have read, that it has been witnessed that this can happen,
but since i tried hard just to get the rhizo part - why am i now
back to normal radial linear mycelium?

i mean, the whole purpose of the transfer was to get the rhizos and
now i am just left with the normal one :/
input?

btw, the rhizo master was from mushroom tissue clone on my beer agar formula,
the transfer was done to PDA plates.
peace ohm :mushroom2:


--------------------
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InvisibleautomanM
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Re: rhizomorphic isolate going back to linear / tomentose [Re: ohmatic]
    #3647094 - 01/19/05 05:19 AM (11 years, 10 months ago)

just give it time and keep transfering until it starts to homogenize.


--------------------
No, no, you're not thinking, you're just being logical. ~ Niels Bohr


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Offlinescatmanrav
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Re: rhizomorphic isolate going back to linear / tomentose [Re: automan]
    #3647103 - 01/19/05 05:32 AM (11 years, 10 months ago)

Whats linear mycelium mean? There are two types...ropey stringy rhizos and cotteny fluffy shit. You have cotteny fluffy shit?


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Invisibleohmatic
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Re: rhizomorphic isolate going back to linear / tomentose [Re: scatmanrav]
    #3647104 - 01/19/05 05:32 AM (11 years, 10 months ago)

linear see page 32 TMC figure 35.

it isnt cottony, it is linear :wink:
peace ohm :mushroom2:

*cough*
i found that on the net...


Edited by ohmatic (01/19/05 05:37 AM)


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Offlinescatmanrav
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Re: rhizomorphic isolate going back to linear / tomentose [Re: ohmatic]
    #3647112 - 01/19/05 05:53 AM (11 years, 10 months ago)

Perhaps I'm incorrect but that picture looks pretty much like what your going for. Guess you'll have to wait for some more experienced with agar...


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Invisibleohmatic
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Re: rhizomorphic isolate going back to linear / tomentose [Re: scatmanrav]
    #3647116 - 01/19/05 05:55 AM (11 years, 10 months ago)

yeah i know that my isolate is actually quite good.
but that's not the question.
deal is, how can this be possible?
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Offlinescatmanrav
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Re: rhizomorphic isolate going back to linear / tomentose [Re: ohmatic]
    #3647121 - 01/19/05 06:00 AM (11 years, 10 months ago)

I'm still confused...the picture looks like rhizos...I guess I'm asking the difference between rhizo growth and linear growth because to me, they seem to be the same thing. Sorry I dont have TMC.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
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Re: rhizomorphic isolate going back to linear / tomentose [Re: scatmanrav]
    #3647127 - 01/19/05 06:07 AM (11 years, 10 months ago)

scatman here we got what i would have expected,
a nice - fat rhizomorphic growth:


actually the sector used for isolation looked alike above rhizos
but well, they somewhat developed back to the linear kind :/
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Offlinescatmanrav
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Re: rhizomorphic isolate going back to linear / tomentose [Re: ohmatic]
    #3647142 - 01/19/05 06:29 AM (11 years, 10 months ago)

It seems that linear is somewhere between rhizo and cottony. When I worked with isolation a bit on agar I found I sometimes would get some of the cotteny mixed in and would get the half and half look of the first pic, maybe a little more ropey though.

I'd say the only possibility was that there was more then the one type of mycelium on each wedge that you took and that it just wasnt very visable yet...but thats just my thoughts to hold you over for the time being until you get some who have done more and better work isolating..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
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Re: rhizomorphic isolate going back to linear / tomentose [Re: scatmanrav]
    #3647151 - 01/19/05 06:37 AM (11 years, 10 months ago)

k, thx for the input, i wonder how the plates are going to develop.
could the change in the agar 'formula' have caused this?
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Offlinehyphae
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Re: rhizomorphic isolate going back to linear / tomentose [Re: ohmatic]
    #3647261 - 01/19/05 08:42 AM (11 years, 10 months ago)

Linear is a very fine rhizo growth pattern not at all tometose. Ps.cyan is known for it's linear growth on agar yet very rhizo on wood chip substrates. I'd give it time the change in substrate has much to do with the difference in growth patterns. GL


--------------------
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Invisibleohmatic
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Re: rhizomorphic isolate going back to linear / tomentose [Re: hyphae]
    #3647263 - 01/19/05 08:43 AM (11 years, 10 months ago)

very interesting.

as soon as i got batteries i will get u updated with pictures from the
agar which i got to say im proud of that i did all the work with just
2 plates contammed in the whole initial cloning, transferring session :smile:

btw.. i think i fell in love with agar =)
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Invisibleagar
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Re: rhizomorphic isolate going back to linear / tomentose [Re: ohmatic]
    #3648272 - 01/19/05 03:10 PM (11 years, 10 months ago)

quote]ohmatic said: could the change in the agar 'formula' have caused this?




I have seen this change many times.

IMHO....& observations Myc can change from one to the other, as it needs to. If all else is equal - excluding the nutes?

Usually this happens because of a change in what it is colonizing. Myc sort of develops BIG TEETH (rhizo) & WALKING SHOES to travel through & digest something tougher (note myc usually turns rhizo - when it hits hard straw, or tough dung clump,) or hits non/nute container wall & tries to travel up it.

At the same time, if it is colonizing something soft & easy to digest & does not have to fight its way through it, it turns linear (LITTLE TEETH). Because it doesn?t need BIG RHIZO TEETH & WALKING SHOES to do so.

You can sometimes witness same thing inside a colonizing wbs/rye glass jar. Often, linear myc colonizes up to the inside glass (no nutes), then turns rhizo (develops big teeth & walking shoes)& travels along the internal glass face.

I would imagine, the change in agar nutes was the cause of it turning from one to the other. That?s my 0.02 cents....


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Invisibletripndicular
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Re: rhizomorphic isolate going back to linear / tomentose [Re: ohmatic]
    #3648450 - 01/19/05 03:39 PM (11 years, 10 months ago)

I agree with the big guy ... agar .
Did the ph in new food source change from original plates . I have noticed this happen several times now . Rh also can make nice ropey rhizos turn cottony or arieal .

PS just a weird ass guess here ... is that PESA ?
Have seen that lots ... PESA on PDA do that more often than not , stick with MAE or wheat based agars and PESA will stay true . That is assuming I am right and you are cloning some PESA .


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Edited by tripndicular (01/19/05 03:45 PM)


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Invisibleohmatic
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Re: rhizomorphic isolate going back to linear / tomentose [Re: tripndicular]
    #3648464 - 01/19/05 03:41 PM (11 years, 10 months ago)

uh i dont have an idea about the ph to be honest,
i could however test it once i liquify it again.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Invisibletripndicular
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Re: rhizomorphic isolate going back to linear / tomentose [Re: ohmatic]
    #3648491 - 01/19/05 03:47 PM (11 years, 10 months ago)

Did you see my edited ps ?
Sorry thought of that afterwards , then saw your response .


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop for: ½ Pint Jars, Agar, Paul Stamets, Potato Dextrose, Rye Grain, Wild Bird Seed, pH Test Strips

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