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tibberous
Stranger

Registered: 12/26/04
Posts: 1,194
Last seen: 4 months, 11 days
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How much better is isolating strains?
#6996171 - 06/01/07 03:44 PM (16 years, 8 months ago) |
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If you had a bunch of spores and were trying to get a bunch of mushrooms, would it be better to make LC's from the spores, or to take the spores, squirt them on agar, wait for the agar to show growth, grab a fast growing, fibrous looking piece and make the LC with that?
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Brainiac
Rogue Scientist



Registered: 04/29/06
Posts: 13,259
Loc: 與您的女朋
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Re: How much better is isolating strains? [Re: tibberous]
#6996182 - 06/01/07 03:49 PM (16 years, 8 months ago) |
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Squirt them on agar, wait for the agar to show growth, grab a fast growing, fibrous looking piece and make the LC with that?

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Fair is Fair
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creamcorn
mad scientist



Registered: 03/13/06
Posts: 2,962
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Re: How much better is isolating strains? [Re: Brainiac]
#6996224 - 06/01/07 04:04 PM (16 years, 8 months ago) |
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isolating strains isn't "automatically" better than growing from spores.
where the advantage comes in, is CHOICE. when isolating strains, you can isolate several, do test grows, and retain the one that works the best. you can then achieve consistent results when working with the same isolate. you can, technically isolate a strain that doesn't even produce mushrooms if you're unlucky or totally ignore the fact that you're looking to isolate the most rhizomorphic growth 
spores don't give you a choice, and your results are much more random in nature.
a procedure that you describe might not even result in a true isolate anyway. you typically have to transfer bits of mycelium to new plates a couple times in succession until you've truly narrowed it down to a single strain (when it no longer splits up, or sectors, into separate growth patterns on a given plate, you know you've got it truly isolated.) if you were to squirt spores on agar, and transfer any given piece of mycelium directly to a liquid culture, you don't know for sure you've actually isolated anything yet. decreased the number of strains, perhaps (and even that seems to have some benefit - less energy is wasted through the grow in combining into a single unified organism with fewer strains in the pot)... but you'll want to do a few transfers to actually isolate a strain.
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Brainiac
Rogue Scientist



Registered: 04/29/06
Posts: 13,259
Loc: 與您的女朋
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Re: How much better is isolating strains? [Re: creamcorn]
#6996233 - 06/01/07 04:07 PM (16 years, 8 months ago) |
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True, I just didn't feel like typeing all of that.
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Fair is Fair
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