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spacecadetCID
CityForager

Registered: 09/01/14
Posts: 109
Last seen: 4 years, 8 months

Re: Stro's Cleaning and Isolating On Agar

[Re: cronicr]
#21559368 - 04/17/15 11:35 AM (11 years, 4 months ago)

Awesome thread! commenting for future reference!

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LIVE FREE!

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Stropharis

Registered: 04/02/07
Posts: 1,854

Re: Stro's Cleaning and Isolating On Agar

[Re: cronicr]
#21559580 - 04/17/15 12:38 PM (11 years, 4 months ago)

spacechildo said:

congrats on the tag Stro! Truly deserved!

cronicr said:

suits ya well;)

:douchewink:

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Current Grows

Stro's Write Ups
The Noob Forum
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blackdust

Registered: 02/28/09
Posts: 8,327

Re: Stro's Cleaning and Isolating On Agar

[Re: Stropharis]
#21562561 - 04/18/15 08:18 AM (11 years, 4 months ago)

How long can a agar plate (non inoculated) sit before contaminating? :crazy:

:crazy:

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bodhisatta
Smurf real estate agent

Registered: 04/30/13
Posts: 62,099
Loc: Milky way

Re: Stro's Cleaning and Isolating On Agar

[Re: blackdust]
#21562569 - 04/18/15 08:22 AM (11 years, 4 months ago)

blackdust said:

How long can a agar plate (non inoculated) sit before contaminating?

Years it will dry out first. If contamination can't get in it can't get in

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Everything you need in one spot.
My Q&A thread. Ask me questions here rather than in Private MessageBod's chat thread
5g a day keeps the 5g away

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blackdust

Registered: 02/28/09
Posts: 8,327

Re: Stro's Cleaning and Isolating On Agar

[Re: bodhisatta]

1
#21562578 - 04/18/15 08:29 AM (11 years, 4 months ago)

Coming from you Bod, I feel pretty safe running a few dozen plates and then inoculate throughout the month. Running my PC can be a challenge so this will help me. Thank you.

---
Yes, i own my house and the misses approves of the grows. Just dont want my babies around the PC or telling grandma. :ooo:

:ooo:

---
I would like to add, I took Whytes advice on getting spores to germinate on agar and it seems to be doing the trick (for my exotics). For anyone who might want to know here it is

1. Make agar softer
2. Take blade (or other tool) and flame then coll the tool in the agar plate to make a little dip. This also get the agar a little wet in the spot.
3. use inoculating loop to transfer spores to the dip

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bodhisatta
Smurf real estate agent

Registered: 04/30/13
Posts: 62,099
Loc: Milky way

Re: Stro's Cleaning and Isolating On Agar

[Re: blackdust]
#21562588 - 04/18/15 08:35 AM (11 years, 4 months ago)

Leave your sab set up and keep the plates in there even so you don't have to wrap unwrap

--------------------
Everything you need in one spot.
My Q&A thread. Ask me questions here rather than in Private MessageBod's chat thread
5g a day keeps the 5g away

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abductee
Time

Registered: 05/07/15
Posts: 2,224
Loc: Canada

Re: Stro's Cleaning and Isolating On Agar

[Re: spacecadetCID]
#21930867 - 07/11/15 09:09 PM (11 years, 1 month ago)

me too

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Dr.Satan
Mad Professor

Registered: 06/26/15
Posts: 2,182

Re: Stro's Cleaning and Isolating On Agar

[Re: abductee]
#21952665 - 07/16/15 04:13 PM (11 years, 1 month ago)

This is very good information +5 :mushroom:

:mushroom:

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Stropharis

Registered: 04/02/07
Posts: 1,854

Re: Stro's Cleaning and Isolating On Agar

[Re: Dr.Satan]
#21956759 - 07/17/15 03:49 PM (11 years, 1 month ago)

:rockon:

Glad I could help!

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abductee
Time

Registered: 05/07/15
Posts: 2,224
Loc: Canada

Re: Stro's Cleaning and Isolating On Agar

[Re: Stropharis]
#21957799 - 07/17/15 08:14 PM (11 years, 1 month ago)

is it just me or does a true monoculture look similar to viewing a mushroom from the bottom?

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Stropharis

Registered: 04/02/07
Posts: 1,854

Re: Stro's Cleaning and Isolating On Agar

1
#21969404 - 07/20/15 06:13 AM (11 years, 1 month ago)

--------------------
Current Grows

Stro's Write Ups
The Noob Forum
Amateur Mycologists United
The Tribe

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abductee
Time

Registered: 05/07/15
Posts: 2,224
Loc: Canada

Re: Stro's Cleaning and Isolating On Agar

[Re: Stropharis]
#21993191 - 07/24/15 11:16 PM (11 years, 27 days ago)

Just reading this over again,and I realized I never formally thanked you for this excellent write up. It has definitely helped re-ignite my love for science and biology.
I wish I had some kind of question, but your write up and question answering covers everything I could think of about isolating.

Thanks :smile:

:smile:

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kiksen

Registered: 06/04/15
Posts: 60
Last seen: 1 month, 12 days

Re: Stro's Cleaning and Isolating On Agar

[Re: abductee]
#21993773 - 07/25/15 05:44 AM (11 years, 27 days ago)

Woow, awesome tek, i cant wait to inuculate my agar dishes, thank you!

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jcop
myself

Registered: 12/02/14
Posts: 163
Loc: Prague, CZ

Last seen: 6 years, 10 months

Re: Stro's Cleaning and Isolating On Agar

[Re: kiksen]
#22046500 - 08/05/15 10:16 AM (11 years, 16 days ago)

Hey, thanks for the write up, gotta get my agar :nyan:

:nyan:

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cronicr

Registered: 08/07/11
Posts: 65,471
Loc: Van Isle

Last seen: 3 months, 27 days

Re: Stro's Cleaning and Isolating On Agar

[Re: jcop]
#22138476 - 08/25/15 01:51 AM (10 years, 11 months ago)

:bump2:

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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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abductee
Time

Registered: 05/07/15
Posts: 2,224
Loc: Canada

Re: Stro's Cleaning and Isolating On Agar *DELETED*

[Re: cronicr]
#22139511 - 08/25/15 07:47 AM (10 years, 11 months ago)

Post deleted by abductee

Reason for deletion: nmp

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DirdyD

Registered: 12/01/14
Posts: 1,650
Last seen: 5 years, 6 months

Re: Stro's Cleaning and Isolating On Agar

[Re: abductee]
#22139828 - 08/25/15 08:58 AM (10 years, 11 months ago)

My go-to reference for the agar work ahead.

This is really top notch material and i'm more than thankful, brother.

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First Outdoor Grow ReduxOutdoor Grow 2

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abductee
Time

Registered: 05/07/15
Posts: 2,224
Loc: Canada

Re: Stro's Cleaning and Isolating On Agar

[Re: DirdyD]

1
#22139924 - 08/25/15 09:32 AM (10 years, 11 months ago)

yeah, this is an awesome write up.

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taGyo
Strainiac/AMU

Registered: 10/16/14
Posts: 18,802
Loc: Journal Land

Last seen: 7 years, 9 months

Re: Stro's Cleaning and Isolating On Agar

[Re: abductee]
#22140043 - 08/25/15 10:06 AM (10 years, 11 months ago)

Don't forget to rate stro,

:super:

:super:

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Gyo's Better Grows
TNF Q&A :rockon:

:rockon:

AMU Q&A

Dominus fortunae meae sum

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abductee
Time

Registered: 05/07/15
Posts: 2,224
Loc: Canada

Re: Stro's Cleaning and Isolating On Agar

[Re: taGyo]
#22140068 - 08/25/15 10:13 AM (10 years, 11 months ago)

I'll click around, not sure how to rate people or what it does. I think I rated someone before though.

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