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Offlinesean123
Stranger
Registered: 04/14/01
Posts: 351
Last seen: 21 years, 5 months
Re: What exactly is agar, and what do u do with it??
    #69440 - 12/23/99 10:53 AM (22 years, 9 months ago)

Hi Spellbin. You need to read some books on mushroom cultivation. Agar is a medium and you put it in petri dishes and you strape spores across it and the spores germinate on the agar in the petri dish and grow into mycelium. then the mycelium is transfered to various substrates or into more petri dishes filled with the agar medium to make more mycelium. i dont know how you are going to grow mushrooms if you dont know what you are doing. get some books for good sakes lol.


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Anonymous

Re: What exactly is agar, and what do u do with it?? [Re: sean123]
    #69442 - 12/23/99 11:15 AM (22 years, 9 months ago)

I would love to read some books on mycology.. heh, just havn't got around to it.. Also, i do have a fair knowlege about mushrooms, using the pf tek method i guess..
Thanks fer telling me..
Cya,
Spelly


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Offlinen8
member
Registered: 12/14/99
Posts: 133
Last seen: 21 years, 1 month
Re: What exactly is agar, and what do u do with it?? [Re: sean123]
    #69443 - 12/23/99 11:25 AM (22 years, 9 months ago)

don't be so harsh on him... the basic teks don't even mention agar, and he said himself he's just starting. damn.

and yeah. it's an advanced culture media thing. i don't see what all the fuss is about unless you're working with truly massive amounts of mushrooms, and even then i don't see how it saves so much time, especially since there are some easier liquid mycelia techniques.

------------------
this time, the revolution will NOT be televised.



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Offlinesean123
Stranger
Registered: 04/14/01
Posts: 351
Last seen: 21 years, 5 months
Re: What exactly is agar, and what do u do with it?? [Re: sean123]
    #69444 - 12/23/99 12:01 PM (22 years, 9 months ago)

i didn't mean to be harsh. but you are right. the PF/MMGG tech does not require agar culturing. sorry

[This message has been edited by sean123 (edited December 23, 1999).]



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Invisibleshane67
Keeper
Registered: 12/01/00
Posts: 113
Re: What exactly is agar, and what do u do with it?? [Re: sean123]
    #69445 - 12/24/99 08:32 AM (22 years, 9 months ago)

Hello!

Check out a CD-ROM called, "How To Grow Shrooms" by Keeper at www.sporetradingpost.com/videoroom.htm

You can't go wrong!

Shane67



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InvisibleLinkA
Intrinsically No Good

Registered: 07/29/99
Posts: 2,314
Loc: Lost Woods, Hyrule
Re: What exactly is agar, and what do u do with it?? [Re: sean123]
    #69446 - 12/24/99 09:46 AM (22 years, 9 months ago)

From what I've read on agar(not much), it's main benefit is beating contamination. If you have a dirty spore print, you can start it on agar, and most likely at least part of it will grow good mycelium. you can then cut that out, and transfer it to a new plate, thus growing only the good stuff.

I'd like to hear more from someone who does it, though, and why they do it.

------------------
Lancelot Link: Secret Chimp



--------------------
Wonder had gone away, and he had forgotten that all life is only a set of pictures in the brain, among which there is no difference betwixt those born of real things and those born of inward dreamings, and no cause to value the one above the other.  -- H.P. Lovecraft "The Silver Key"


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Offlineyounger now
old hand
Registered: 07/17/99
Posts: 243
Last seen: 20 years, 6 months
Re: What exactly is agar, and what do u do with it?? [Re: sean123]
    #69447 - 12/26/99 06:56 AM (22 years, 9 months ago)

Good Q Spell, I was kinda wondering about that myself but I looked through ALL the tec.'s first trying to find more on it. You got around to asking before I did. There isn't a lot of beginners info on it at all.


--------------------
"My Brain Hurts!!!!".....Monty Python :crazy:


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OfflineWorkmanV
Psilocybe Microscopist
Male User Gallery

Registered: 03/01/01
Posts: 3,566
Loc: Washington, USA
Last seen: 3 years, 9 months
Trusted Cultivator
Re: What exactly is agar, and what do u do with it?? [Re: sean123] * 3
    #69448 - 12/26/99 06:57 PM (22 years, 9 months ago)

I use agar and I love it.

The idea is that you are growing on a 2-dimensional plane (the agar surface) instead of a 3-dimensional substrate (PF cake, grain in jars, etc.) On the flat surface of the agar, contaminates cannot hide from you. As Link said, you can isolate clean mycelium from dirty prints. You can also clone fragments of tissue, do breeding experiments by isolating single spores and select and propogate specific isolates. Agar is also often used for the storage of favored strains. Just pop them in the frig for several months.

Agar culture is just a more powerful and precise way of doing things. If you like control, agar is for you. If not, just inject your spores and cross your fingers :wink:



--------------------
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Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


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