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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Contaminated Agar...what is this shit! (pics) * 6
    #73307 -

i think you spilled cheese puffs in your agar

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Re: Contaminated Agar...what is this shit! (pics) * 1
    #73309 -

The yellow blotch I have seen before. It's pretty common, smells like rotting apples. You must have some problem with something, and my guess is media. How long do you sterlize your agar for? You should do it for 30-40 mins, in a flask with a cotton swab stuffed in the neck and tin foil on top. You should let you pressure cooker cool down normally and then put a paper towel soaked with alcohol or Lysol over the stopcock/neck to prevent contaminents from entering as it cools. Other than that, just wipe your tools, surfaces and gloves with alcohol or 15% bleach/water mix.

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Re: Contaminated Agar...what is this shit! (pics)
    #73310 -

Those are bacterial contams
I have trouble with those.

1. Sterilise the media properly
2. Alcohol isn't good enough for bacteria
3. Use Lysol

No 1 is the most important since depending on the media some pretty heat resistant bact. contams exist.

Do many dishes and that way u are assured of a few clean ones.


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Re: Contaminated Agar...what is this shit! (pics)
    #73311 -

.


--------------------
-Peace-

High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com



muShrooms.com is the new web site of
www.thehawkseye.com

Edited by Ryche Hawk (06/05/17 08:15 PM)

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Re: Contaminated Agar...what is this shit! (pics)
    #73312 -

Does adding gentamycin (an anti-biotic additive) to agar ala Smart Botanics work well?

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Re: Contaminated Agar...what is this shit! (pics)
    #73313 -

That's an intersting idea with the drying Ryche Hawk. I don't have a flow hood, but I did a lot of investigation on this condensation issue, and here's what i have found.

Stacking your dishes helps a hell of a lot. The uppermost dish (heat rises of course) will have the worst effects, so just sacrifice this one. Also leaving warm water in the top dish helps too. I usually incubate my petris upside down, this is a fairly common lab practise and helps a lot.
Also like everyone has said, leaving the agar untill it is nearly gelled before pouring will help.


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Re: Contaminated Agar...what is this shit! (pics)
    #73314 -

I defintely remember some post about 3 months ago concerning using Peroxide in agar. I tried the search engine but gave up after 5 mins. of waiting. Good luck!

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Re: Contaminated Agar...what is this shit! (pics)
    #73315 -

Thanks to everyone that responded to my post.

Ryche Hawk...that is it!

A lot of water has gotten into my petri dishes. I also have been pouring the Agar when it is just cool enough to handle (very hot). I will try again tonight (just recently got a flask w/glass stopper).

I am going to pick-up some yeast for a mycelium brew tonight?wish me luck!


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Re: Contaminated Agar...what is this shit! (pics)
    #73316 -

.


--------------------
-Peace-

High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com



muShrooms.com is the new web site of
www.thehawkseye.com

Edited by Ryche Hawk (06/05/17 08:15 PM)

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Re: Contaminated Agar...what is this shit! (pics)
    #73317 -

Guys & Gals

When I first started growing I had almost 100% non-contamination rate (glove box & foil to wrap my dishes). Now that I have a flow hood I feel I didn't need to use foil (which is rightly so). Well, I am going back to the foil. I recall no extra moisture build-up after autoclave and had great results.


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Re: Contaminated Agar...what is this shit! (pics)
    #73318 -

What kind of HEPA are you using? is htis the furnace HEPA or the LAB HEPA?

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Re: Contaminated Agar...what is this shit! (pics)
    #73319 -

Gainen,

I am using the Lab HEPA. A matter-a-fact I wrapped my petri dishes in foil and allowed the Agar to cool...not a single contamination! Thank you Ryche Hawk and everyone that helped me solve my problem!


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Re: Contaminated Agar...what is this shit! (pics)
    #73320 -

Good ideas Rhyche. I am having some serious probs with contams right now. I don't have any good prints either which doesn't help (ordered one from you though). I might try using the tin foil, I was using plastic wrap but it doesn't seem to be doing the job. I agree with Rhyche though, for some reason once you have mycelium condensation doesn't seem to be a problem. I am getting to the point where I might just do a multi spore into rye just to get a healthy mycelium. ARG!

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Re: Contaminated Agar...what is this shit! (pics)
    #73321 -

Just noticed that I spelled Ryche wrong. : )

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Re: Contaminated Agar...what is this shit! (pics)
    #73322 -

.


--------------------
-Peace-

High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com



muShrooms.com is the new web site of
www.thehawkseye.com

Edited by Ryche Hawk (06/05/17 08:15 PM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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