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Anonymous

Re: Contaminated Agar...what is this shit! (pics) * 1
    #73307 - 01/31/00 05:09 AM (22 years, 7 months ago)

i think you spilled cheese puffs in your agar


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Anonymous

Re: Contaminated Agar...what is this shit! (pics) * 1
    #73309 - 01/31/00 05:51 AM (22 years, 7 months ago)

The yellow blotch I have seen before. It's pretty common, smells like rotting apples. You must have some problem with something, and my guess is media. How long do you sterlize your agar for? You should do it for 30-40 mins, in a flask with a cotton swab stuffed in the neck and tin foil on top. You should let you pressure cooker cool down normally and then put a paper towel soaked with alcohol or Lysol over the stopcock/neck to prevent contaminents from entering as it cools. Other than that, just wipe your tools, surfaces and gloves with alcohol or 15% bleach/water mix.


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Anonymous

Re: Contaminated Agar...what is this shit! (pics)
    #73310 - 01/31/00 12:32 PM (22 years, 7 months ago)

Those are bacterial contams
I have trouble with those.

1. Sterilise the media properly
2. Alcohol isn't good enough for bacteria
3. Use Lysol

No 1 is the most important since depending on the media some pretty heat resistant bact. contams exist.

Do many dishes and that way u are assured of a few clean ones.



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InvisibleRyche HawkV
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Re: Contaminated Agar...what is this shit! (pics)
    #73311 - 02/04/00 08:09 PM (22 years, 7 months ago)

.


--------------------
-Peace-

High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com



muShrooms.com is the new web site of
www.thehawkseye.com


Edited by Ryche Hawk (06/06/17 03:15 AM)


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Anonymous

Re: Contaminated Agar...what is this shit! (pics)
    #73312 - 02/04/00 09:26 PM (22 years, 7 months ago)

Does adding gentamycin (an anti-biotic additive) to agar ala Smart Botanics work well?


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Anonymous

Re: Contaminated Agar...what is this shit! (pics)
    #73313 - 02/04/00 10:25 PM (22 years, 7 months ago)

That's an intersting idea with the drying Ryche Hawk. I don't have a flow hood, but I did a lot of investigation on this condensation issue, and here's what i have found.

Stacking your dishes helps a hell of a lot. The uppermost dish (heat rises of course) will have the worst effects, so just sacrifice this one. Also leaving warm water in the top dish helps too. I usually incubate my petris upside down, this is a fairly common lab practise and helps a lot.
Also like everyone has said, leaving the agar untill it is nearly gelled before pouring will help.



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Anonymous

Re: Contaminated Agar...what is this shit! (pics)
    #73314 - 02/04/00 10:47 PM (22 years, 7 months ago)

I defintely remember some post about 3 months ago concerning using Peroxide in agar. I tried the search engine but gave up after 5 mins. of waiting. Good luck!


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Invisibleshane67
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Registered: 12/01/00
Posts: 113
Re: Contaminated Agar...what is this shit! (pics)
    #73315 - 02/04/00 11:26 PM (22 years, 7 months ago)

Thanks to everyone that responded to my post.

Ryche Hawk...that is it!

A lot of water has gotten into my petri dishes. I also have been pouring the Agar when it is just cool enough to handle (very hot). I will try again tonight (just recently got a flask w/glass stopper).

I am going to pick-up some yeast for a mycelium brew tonight?wish me luck!



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InvisibleRyche HawkV
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Re: Contaminated Agar...what is this shit! (pics)
    #73316 - 02/05/00 08:54 AM (22 years, 7 months ago)

.


--------------------
-Peace-

High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com



muShrooms.com is the new web site of
www.thehawkseye.com


Edited by Ryche Hawk (06/06/17 03:15 AM)


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Invisibleshane67
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Posts: 113
Re: Contaminated Agar...what is this shit! (pics)
    #73317 - 02/05/00 04:55 PM (22 years, 7 months ago)

Guys & Gals

When I first started growing I had almost 100% non-contamination rate (glove box & foil to wrap my dishes). Now that I have a flow hood I feel I didn't need to use foil (which is rightly so). Well, I am going back to the foil. I recall no extra moisture build-up after autoclave and had great results.



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Anonymous

Re: Contaminated Agar...what is this shit! (pics)
    #73318 - 02/06/00 07:47 AM (22 years, 7 months ago)

What kind of HEPA are you using? is htis the furnace HEPA or the LAB HEPA?


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Invisibleshane67
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Registered: 12/01/00
Posts: 113
Re: Contaminated Agar...what is this shit! (pics)
    #73319 - 02/09/00 09:12 PM (22 years, 7 months ago)

Gainen,

I am using the Lab HEPA. A matter-a-fact I wrapped my petri dishes in foil and allowed the Agar to cool...not a single contamination! Thank you Ryche Hawk and everyone that helped me solve my problem!



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Anonymous

Re: Contaminated Agar...what is this shit! (pics)
    #73320 - 02/09/00 10:57 PM (22 years, 7 months ago)

Good ideas Rhyche. I am having some serious probs with contams right now. I don't have any good prints either which doesn't help (ordered one from you though). I might try using the tin foil, I was using plastic wrap but it doesn't seem to be doing the job. I agree with Rhyche though, for some reason once you have mycelium condensation doesn't seem to be a problem. I am getting to the point where I might just do a multi spore into rye just to get a healthy mycelium. ARG!


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Anonymous

Re: Contaminated Agar...what is this shit! (pics)
    #73321 - 02/09/00 10:59 PM (22 years, 7 months ago)

Just noticed that I spelled Ryche wrong. : )


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InvisibleRyche HawkV
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Re: Contaminated Agar...what is this shit! (pics)
    #73322 - 02/13/00 06:08 AM (22 years, 7 months ago)

.


--------------------
-Peace-

High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com



muShrooms.com is the new web site of
www.thehawkseye.com


Edited by Ryche Hawk (06/06/17 03:15 AM)


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