Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineangelko
Stranger
Registered: 04/23/03
Posts: 11
Last seen: 18 years, 4 months
Pour agar into petri dishes before pc
    #1555860 - 05/17/03 08:03 AM (20 years, 10 months ago)

Can agar solution be poured into petri dishes and then pc the petri dishes instead of pouring after the agar has been sterilized? Allow the petri dishes to cool and then inoculate.

I thought this might avoid the possible contamination possible during pouring the dishes after the agar has been sterilized.

Any reason why this wouldn't work?

Thanks

Extras: Filter Print Post Top
InvisiblePrisoner#1
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Pour agar into petri dishes before pc [Re: angelko]
    #1555877 - 05/17/03 08:17 AM (20 years, 10 months ago)

Yes...it's a little better way of doing it but you'll need some sort of rack to hold the dishes while in the PC, allow it to cool in the PC and make sure that you use a flow hood when streaking the plates....with the use of a flow hood you can pour them after autoclaving....

P#1

Extras: Filter Print Post Top
Invisiblepoke smot!
floccinocci floofinator
Male

Registered: 01/08/03
Posts: 5,248
Re: Pour agar into petri dishes before pc *DELETED* [Re: Prisoner#1]
    #1555937 - 05/17/03 09:29 AM (20 years, 10 months ago)

Post deleted by poke smot!

Reason for deletion: x


Extras: Filter Print Post Top
InvisiblePrisoner#1
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Pour agar into petri dishes before pc [Re: poke smot!]
    #1555963 - 05/17/03 09:59 AM (20 years, 10 months ago)

I seldom have problems with contamination either, usualy I have to introduce them but the average joe needs a little assist sometimes from the HEPA gods....you have to lear and then practice for good techniques....not many people are born with it....

Extras: Filter Print Post Top
Offlineangelko
Stranger
Registered: 04/23/03
Posts: 11
Last seen: 18 years, 4 months
Re: Pour agar into petri dishes before pc [Re: Prisoner#1]
    #1556069 - 05/17/03 11:24 AM (20 years, 10 months ago)

What does this mean "usualy I have to introduce them.."?

Extras: Filter Print Post Top
InvisiblePrisoner#1
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Pour agar into petri dishes before pc [Re: angelko]
    #1558200 - 05/18/03 05:05 PM (20 years, 10 months ago)

meaning, that I have been cultivating contaminants...I got a little bored several months back and decided to try something different, I originaly started with cobweb mold in a myco bag on rye grain....using the same technique that most people would use for cloning, I have taken the stronger cultures and propagated them....the end result...a very dificult to kill cobweb mold, not a delicate form, it's tougher than pulling apart polyfill.....my Trichoderma aggressivum, aka. the green mold...it seems to have a difficult time fighting off the bacteria. my black mold did not fare well against the mycelium yet...but I have hope....

I became very acustomed to using clean techniques for inocculation of plates and handling many of the cultures, honestly, I dont worry much about contams anymore, I have actualy contaminated many of my cultures in order to try and kill the contaminants...ok...so I'm weird....but I've learned a great deal about these things.....

P#1

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* Re: Agar & Petri Dish Problem MrBoomer 2,284 3 04/16/01 09:49 PM
by Anno
* premade petri dishes? High_On_Magic 1,930 4 09/15/03 04:10 PM
by simplemachine
* Re: Petri Dishes & Agar Q's Placebo 4,007 5 12/07/99 10:00 AM
by Anonymous
* How is the best way to pressure cook Petri Dishes?
( 1 2 all )
SafeHaven 16,195 20 12/09/23 10:27 PM
by vazap
* Some of my first petri dishes ever :) kykeon 3,152 7 10/26/02 12:24 AM
by kykeon
* Temp for pouring agar? oscill8 2,868 1 09/19/01 04:34 PM
by oscill8
* sterilizing petri dishes llamaboy 10,812 11 01/17/04 02:45 PM
by ragadinks
* agar and petri dishes Thworp 2,712 7 01/08/02 08:29 AM
by ar393

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
6,856 topic views. 29 members, 147 guests and 98 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.007 seconds on 12 queries.