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InvisibleDarkmoon
Thinker

Registered: 11/18/99
Posts: 218
Contamination?? any suggestions? *PICS*
    #743341 - 07/13/02 09:37 AM (14 years, 7 months ago)

ok well on my first attempt on 7/9 I pressure cooked 10 1/2 pints. Today when I checked on them I found 5 with definate signs of contamination. I didnt tape my holes though because I was using an incubator and remember reading that the dry verm layer is very good at keep out contams. My question is, can someone give me a clue to what kind of contam this is??

Sorry for the blurry closeups...







I mean i would just like to know if perhaps it looks like something thats common when you contam during innoc, or if perhaps it was due to, too much H2O?? Maybe it was just my sterilaztion? Any ideas or suggestions on this is GREATLY appreciated. Thanks!

oh by the way sorry for posting in the wrong forum, thanks moe



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OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 1 month, 22 days
Re: Contamination?? any suggestions? *PICS* [Re: Darkmoon]
    #743360 - 07/13/02 09:48 AM (14 years, 7 months ago)

I donno what the conamination is, but it looks like it has entered each jar around the innoculation point. My guess is that you have a contaminated syringe rather than a problem with water balance or general sterilization techniques.


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InvisibleDarkmoon
Thinker

Registered: 11/18/99
Posts: 218
Re: Contamination?? any suggestions? *PICS* [Re: Seuss]
    #743369 - 07/13/02 09:54 AM (14 years, 7 months ago)

yea, it does appear that way. however that is actualy 2 strains pictured. so do you think this would mean I contaminated both syringes at some point during the innoc. unfortunatlly i didnt have an alcohol lamp to steralize the needle and i used a lighter and wiped clean with 93% alcohol....would the soot left by the lighter if there was any do it? Thnaks again!!


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Offlinemew
member
Registered: 05/09/01
Posts: 151
Last seen: 14 years, 4 months
Re: Contamination?? any suggestions? *PICS* [Re: Darkmoon]
    #749558 - 07/15/02 11:52 PM (14 years, 7 months ago)

Prolly not but the innoculate in the syringes their selves may be contamed .


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Invisiblemickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: Contamination?? any suggestions? *PICS* [Re: Darkmoon]
    #767614 - 07/22/02 02:25 PM (14 years, 7 months ago)

Using a lighter, and then wiping with alchohol should be enough to sterilize the needle. I'm certain the contamination was inside the syringe itself. If you bought the syringes, get a replacement. If you made them, be more sterile next time.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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InvisibleDarkmoon
Thinker

Registered: 11/18/99
Posts: 218
Re: Contamination?? any suggestions? *PICS* [Re: mew]
    #769773 - 07/23/02 08:58 AM (14 years, 7 months ago)

I do have some jars from the first batch though that do look to be doing good. If the contam was inside the syringe is it possible for thoses to be contaminaded even though they dont show it?

Or does it mean perhaps that the spores beat the contam in some of the jars? I can post pics of the ones doind good if you would like to see. Thanks again everyone....


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Offlinerosewoodpete
a grain of sandamongst thedunes

Registered: 05/11/02
Posts: 1,481
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Re: Contamination?? any suggestions? *PICS* [Re: Darkmoon]
    #769935 - 07/23/02 10:20 AM (14 years, 7 months ago)

why is your foil under the lids??? I bet something snuck in because of your procedure.


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InvisibleDarkmoon
Thinker

Registered: 11/18/99
Posts: 218
Re: Contamination?? any suggestions? *PICS* [Re: rosewoodpete]
    #770009 - 07/23/02 10:52 AM (14 years, 7 months ago)

just thought that would be another barrier to contams. i tried some with foil under lids and some without and both have done good and bad???


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Invisibledownforpot
Stranger
Male
Registered: 06/25/01
Posts: 5,715
Re: Contamination?? any suggestions? *PICS* [Re: Darkmoon]
    #770682 - 07/23/02 03:07 PM (14 years, 7 months ago)

black mold, stay away from that shit. it can fuck you up, for life.


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"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"


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Amazon Shop for: ½ Pint Jars, Vermiculite

Mushrooms, Mycology and Psychedelics >> Contamination Forum

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