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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Stro's Cleaning and Isolating On Agar [Re: mrmazdarx9]
    #23691162 -

Learned alot from this, thanks.

B:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #23945551 -

Great stuff thanks!
:takingnotes:


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:jah:Easy as Fuck Isopropyl Hash Oil:jah:


Psilocybe quebecensis


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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #24454692 -

That 'C' culture is a thing of beauty!


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My mini unmod monotub grow log!


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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #24592365 -

so do I understand right that to get a nice transfer I should only cut the outer part of the myc?
I always get better and better rhizo myc when transfered and all of a sudden the next transfer gets worse.
I have always cut like the yellow triangle, but should I cut like square black like in the pic??


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Psy&Goa

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Re: Stro's Cleaning and Isolating On Agar [Re: Midnight Cyclone]
    #24660719 -

this was precisely what I needed. I gonna get serious.


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If you see Lil'ktu then tell her I'm sorry.
Texas Climate and Soil Data

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Re: Stro's Cleaning and Isolating On Agar [Re: Qayyn Eb-Ur] * 1
    #24662748 -

Yea this write up got me going as well.

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Re: Stro's Cleaning and Isolating On Agar [Re: abductee]
    #24810165 -

Thanks for the explanation about sectors.

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Re: Stro's Cleaning and Isolating On Agar [Re: seekinglight] * 1
    #24817459 -

hell yeah cool i was looking for this. thankk u

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Re: Stro's Cleaning and Isolating On Agar [Re: mycoshroom1994]
    #24854684 -

:popcorn:

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #24897404 -

Hello. Necrobump here. Getting ready to start up my agar adventure and was wondering if this is all still viable info. Seems legit. I've read a lot of stuff over and over and this seems to line up.


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Big Newb with lots of questions

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Re: Stro's Cleaning and Isolating On Agar [Re: AshSoul] * 1
    #24897486 -

This post is legit.  :thumbup:


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #24988685 -

If you have cloned a mono culture then grow it out will the spores from the fruit be isolated at all? Or do you just have a bunch of random spores like from most other prints?

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Re: Stro's Cleaning and Isolating On Agar [Re: Chaz08]
    #24988718 -

Random.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Stro's Cleaning and Isolating On Agar [Re: hamloaf]
    #24990717 -

if the plate is bacterial and swampy...

is there any way to salvage the myc in there?
maybe adding something, like, some antibiotics, perhaps, to the receiving plate?


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Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: โ€œAll CONTAMINATED things and events are unsatisfactory.โ€'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

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Re: Stro's Cleaning and Isolating On Agar [Re: pablokabute]
    #24990790 -

There needs to be a leading edge that's not encompassed by bacteria in order for transferring away to work.  If the plate is as bad as described, consider giving Josex's biopsy method a try.
https://www.shroomery.org/forums/showflat.php/Number/24806569#24806569


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Stro's Cleaning and Isolating On Agar [Re: hamloaf]
    #25043779 -

Should we cool the scalpel in the old dish? or the new dish?

New dish is guaranteed clean, but cooling it there would involve opening it twice.

Cooling in the old dish potentially contaminates the scalpel if we cool it in the wrong place.

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Re: Stro's Cleaning and Isolating On Agar [Re: DoDecaPus]
    #25043799 -

Old dish. The heat probably kills any bad shit anyway.


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๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Re: Stro's Cleaning and Isolating On Agar [Re: DoDecaPus]
    #25044091 -

DoDecaPus said:
Should we cool the scalpel in the old dish? or the new dish?

New dish is guaranteed clean, but cooling it there would involve opening it twice.

Cooling in the old dish potentially contaminates the scalpel if we cool it in the wrong place


I cool scalpel in old dish, but have been known to cool scalpel in new dish.  New dish, old dish, doesn't really matter.  Both should be clean.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Stro's Cleaning and Isolating On Agar [Re: hamloaf]
    #25044647 -

Anyone can help me out.. so whats the best way to store your leftover liquid LME(I bought Coopers)? thank you. I hope its ok to ask it here as Stro called for LME in his recipe..

:smile:


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Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: โ€œAll CONTAMINATED things and events are unsatisfactory.โ€'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

Edited by pablokabute (03/07/18 07:07 AM)

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Re: Stro's Cleaning and Isolating On Agar [Re: pablokabute]
    #25046920 -

pablokabute said:
Anyone can help me out.. so whats the best way to store your leftover liquid LME(I bought Coopers)? thank you. I hope its ok to ask it here as Stro called for LME in his recipe..

:smile:


In the container from which you poured it, in the fridge? You'll have to pressure cook it again to bring it up to temperature and kill anything that might have gotten in during ues/storage.

The best policy is probably not to have any left over. After some practice, and getting your pours consistent, the goal is to make just the right amount to fill all the plates in a sterile sleave.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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