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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Stro's Cleaning and Isolating On Agar [Re: cronicr]
    #19673668 -

cronicr said:
Stropharis said:
Just stopping by, I'm hoping to be back at it in March, we will see.


it's march, i didn't forget lol! bumpity bump stropharis where ya at!


I LOL'D! I'm in the shadows Breh!  Lately I can't help myself though and fungus is on the mind.


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Current Grows

Stro's Write Ups
The Noob Forum
Amateur Mycologists United
The Tribe

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #19673861 -

:toast:


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #19876913 -

I know this is old but I want ithe thread, thanks man nice pics :thumbup:

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #20001359 -

wow man nice write-up!
i just jumped into agar and you've explained it very well
THAnx A10T
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:


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Re: Stro's Cleaning and Isolating On Agar [Re: cArcace-x]
    #20001543 -

WOW!!! Very informative & thorough thread...Thank You Stro for taking the time.


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"A spook story that criminals tell their kids at night."
                                                Roger "Verbal" Kint

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Re: Stro's Cleaning and Isolating On Agar [Re: Keyser Soze]
    #20047148 -

Awesome write up Stro!

Would it be best to put spores, a pin, or the inside of a fruit body onto agar?  I haven't found much info on this and it's still a little confusing to me.

Thank you!


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My favorite threads


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Re: Stro's Cleaning and Isolating On Agar [Re: magicMerlin] * 1
    #20057794 -

magicMerlin said:
Awesome write up Stro!

Would it be best to put spores, a pin, or the inside of a fruit body onto agar?  I haven't found much info on this and it's still a little confusing to me.

Thank you!



If you already have a multispore grow going and you want to start working with clones and/or monocultures, then it would be best to transfer a live tissue culture to agar. This clone culture would be the same genetically. However, it will more than likely still carry multiple sectors and not be a true isolate, and it also more than likely won't need to be in order to preform well.


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Current Grows

Stro's Write Ups
The Noob Forum
Amateur Mycologists United
The Tribe

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #20058076 -

Thanks, stro

:tripping:


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Re: Stro's Cleaning and Isolating On Agar [Re: rohr]
    #20066524 -

First of all I want to say THANK YOU, nice write up and I learned that what I thought I understood was wrong. Thanks again for the knowledge.

I have a question:

Is it possible to get a non fruiting strain from and "cloned" isolate?

Edited by Knothead (05/31/14 08:24 PM)

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Re: Stro's Cleaning and Isolating On Agar [Re: Knothead]
    #20066538 -

Knothead said:
Is it possible to get a non fruiting strain from and isolate?


yes, this is why they need to be tested


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Stro's Cleaning and Isolating On Agar [Re: Knothead]
    #20066539 -

I hope Stro doesn't mind me jumping in here to answer that, but yes, it's entirely possible.  You should check out pussyfart's 10 PE ISOLATES thread to see what the end result of isolation by a method very similar to this one can be.


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Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Re: Stro's Cleaning and Isolating On Agar [Re: cronicr]
    #20066590 -

I edited the question, I meant to say "cloned" isolate. Sorry!

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Re: Stro's Cleaning and Isolating On Agar [Re: Knothead]
    #20066603 -

Do you mean an isolate from a clone or a clone of an isolate?


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Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Re: Stro's Cleaning and Isolating On Agar [Re: Psilicon]
    #20066610 -

Thanks Pussyfart, I read that a while back and didn't catch they where cloned isolates. Not trying to rub yall wrong, sometimes us all farts just don't on catch everything the first time. Thanks for the feedback as always.

Another misconception that I had. Thought I understood and found out I didn't.

Sure it's never happened to you. LOL

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Re: Stro's Cleaning and Isolating On Agar [Re: Psilicon]
    #20066615 -

I'm referring to an isolate from a clone, Pussfart provided the answer I think.

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Re: Stro's Cleaning and Isolating On Agar [Re: Psilicon]
    #20066645 -

Sorry VDG, thanks for pointing me to the answer on Pussyfats post! Appreciate the response, confused again.

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Re: Stro's Cleaning and Isolating On Agar [Re: Knothead]
    #20066664 -

all isolates even from clones need to be tested, it';s rare to happen but it is possible  to get a non fruiter from a clone


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Stro's Cleaning and Isolating On Agar [Re: cronicr]
    #20066724 -

Thanks Chonicr, another misconception I had, haven't experienced it, thought I understood something and found out I was wrong.

Live, pay attention and learn.

Thanks to all!

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Re: Stro's Cleaning and Isolating On Agar [Re: Knothead]
    #20137567 -

This is exactly what I needed as I venture onward into my quest of cloning and isolation.  Bravo, very well written and easily digested.

:takingnotes:
:vineclimb:


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:1up:Improved melted SFD/lid tek:1up:Dilated's Ironed Polyfil Filters for Monotubs:1up:

:1up:Dilated's Ironed Polyfil Filters:1up:
:1up:DiaLiners:1up:

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Re: Stro's Cleaning and Isolating On Agar [Re: Dilated]
    #20137779 -

Dilated said:
This is exactly what I needed as I venture onward into my quest of cloning and isolation.  Bravo, very well written and easily digested.

:takingnotes:
:vineclimb:


QFT...im pretty sure this thread alone has helped more ppl than stropharis can imagine...


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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