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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #26408973 -

Great stuff, just what I needed today!

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Re: Stro's Cleaning and Isolating On Agar [Re: dionysiusnextdoor]
    #26432272 -

I found this thread to be extremely helpful. Well done Stro. Cheers!

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Re: Stro's Cleaning and Isolating On Agar [Re: Ray Charles]
    #26444373 -

This thread has been enlightening. :yeahgoodadvice:

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Re: Stro's Cleaning and Isolating On Agar [Re: TIS87]
    #26444532 -

:takingnotes:


--------------------
Tek's I use

LAGM2020

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Re: Stro's Cleaning and Isolating On Agar [Re: staytrippy420]
    #26444743 -

So if myc grows over a bacteria blob how safe is it to transfer from it after it’s grown over and out past the bacteria?? It looks clean but I’m worried some bacteria will be stuck to the myc from when it grew over the colony.



Guess I could just take some and experiment.


--------------------
LAGM2020

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Re: Stro's Cleaning and Isolating On Agar [Re: A.k.a]
    #26444751 -

I wouldn’t trust it but if you got the plates and agar ready why not? :shrug:
I always assumed once it touched any contam it was game over lol


--------------------
Tek's I use

LAGM2020

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Re: Stro's Cleaning and Isolating On Agar [Re: staytrippy420]
    #26444763 -

That pic is a few days old too the circles now are way past the bacteria, but I’ve also assumed it was no good.

I have another plate that swab was in first and it was totally covered in bacteria. A while after pulling it out the bacteria seemed to dry up and now mycelium is sprouting from it.

Idk if bacteria dies eventually or what but even with circular satellite colonies I’ve noticed they usually change after a while, look less watery and more solid.


--------------------
LAGM2020

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Re: Stro's Cleaning and Isolating On Agar [Re: A.k.a]
    #26447737 -

Thank you for the hard earned knowledge my friend thank you

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Re: Stro's Cleaning and Isolating On Agar [Re: truthseeker8987]
    #26463896 -

Thank you for your post Stro


--------------------
I am getting back into growing.

Been experimenting with an electric pressure cooker. 11.6psi for 2 hours seems to work.

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Re: Stro's Cleaning and Isolating On Agar [Re: Disscard]
    #26494232 -

such a good resource here

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Re: Stro's Cleaning and Isolating On Agar [Re: woofwoof]
    #26501179 -

Great write up!!!

I have 2 questions so anyone more experienced than me please feel free to assist me :smile:)

1. Would it matter if I clone a fruit from the first flush (would the clone also have potential to be an early bird) or later flush (would a clone from a later flush have prerequisites to also be a late bloomer?

2. If the best fruit cluster desirable to clone would be on the bottom of the substrate (maybe a stupid question but here it comes) would a clone from that cluster be more prone to grow on the bottom of a substrate as well or just where the surface conditions happens to be most suitable?

Cheers!

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Re: Stro's Cleaning and Isolating On Agar [Re: Kontiki]
    #26501264 -

IMO
1) generally clones are first flush, and of the fastest to pin and/or cluster. People have had great clones from a second flush though.
2) cluster location doesn't matter, surface conditions are reason they are on sides or bottom


--------------------

Crack's Tek's and Shit


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. [Re: Stropharis]
    #26501930 -

.


--------------------

“Hey, nice marmot.” — The Dude

Edited by R3dstaffy (07/23/20 05:18 PM)

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Re: Stro's Cleaning and Isolating On Agar [Re: R3dstaffy]
    #26502091 -

So I just want to add. Sometimes bumping an old thread can be a good thing. This was my go to for agar years ago and still is.

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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
    #26646463 -

Say you're getting tomentose/fuzzy myc on your first plate after inoculating from a print to agar, and also on your transfer plate. Does further isolating allow a more healthy myc to grow? Or will it remain about the same and just ensure a clean isolation? It colonizes grain just fine, but wondering how to get a more rhizomorphic plate to isolate a monoculture.  Such as the ones you have pictured.

I'm not sure if it is the spores themselves or if it just needs further isolation.

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Re: Stro's Cleaning and Isolating On Agar [Re: Humlo]
    #26647897 -

Humlo said:
Say you're getting tomentose/fuzzy myc on your first plate after inoculating from a print to agar, and also on your transfer plate. Does further isolating allow a more healthy myc to grow? Or will it remain about the same and just ensure a clean isolation? It colonizes grain just fine, but wondering how to get a more rhizomorphic plate to isolate a monoculture.  Such as the ones you have pictured.

I'm not sure if it is the spores themselves or if it just needs further isolation.


I've seen rhizo form when u transfer further starting from tomentose. Using less nutrients when making your agar helps with rhizo because it will search for food

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Re: Stro's Cleaning and Isolating On Agar [Re: ouuwee]
    #26650852 -

Awesome, thanks for the info!  I read the other day about limiting nutrients and couldn't figure out why it would have that effect.  Now it makes total sense :thumbup:

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Re: Stro's Cleaning and Isolating On Agar [Re: Humlo]
    #26664301 -

good work

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Re: Stro's Cleaning and Isolating On Agar [Re: lamama]
    #26664347 -

amazing article

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Re: Stro's Cleaning and Isolating On Agar [Re: lamama]
    #26664349 -

good luck

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