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Amazon Shop: ½ Pint Jars, Agar, Petri Dish

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OfflineTheShroomHermit
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Ps. Cyan without agar work?
    #1429724 - 04/04/03 08:25 PM (15 years, 1 month ago)

Bob asked me how Jane would go about cultivating from a wild Ps. Cyan print without working with agar.
Can you help me help him help her out?


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InvisiblePrisoner#1
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Re: Ps. Cyan without agar work? [Re: TheShroomHermit]
    #1431295 - 04/05/03 02:25 PM (15 years, 1 month ago)

Tell Bob that janes project is doomed....always run wild prints on agar...

P#1


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OfflinePaid
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Re: Ps. Cyan without agar work? [Re: Prisoner#1]
    #1431333 - 04/05/03 02:44 PM (15 years, 1 month ago)

If you dont have agar to work with then i would use half the print on a
jar of grain or 2 and the other half in a honey water tec adding h2o2 after germination if there are any impuritys.Good luck with it.


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OfflineDatrutankdogg69
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Re: Ps. Cyan without agar work? [Re: Paid]
    #1431430 - 04/05/03 03:17 PM (15 years, 1 month ago)

Well, Bob an Jane I know that when I picked a pound of them last year I dumped all the spore prints I didn't sale and all the sporewater from rinsing that pound onto a dumptruck full of woodchips that got dumped in mah yard. Well it's a year later an still not completley colonised(about 3/4 or more). So now I've gotta move an I'm stuck with a couple hundred pounds of spawn thats just getting bigger as we speak. So I hope to expand with alder and get some real fruits All that spawn an all I got was an oz or 2. ALOT of aborts an imature and some were growing into tha ground! I'm thinking this is because of the high sap content. I used mostly pine cause in was free.. but if I expand to alder.. AaahhhhIcan'twait...


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InvisibleOlgualion
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Re: Ps. Cyan without agar work? [Re: Paid]
    #1431560 - 04/05/03 04:12 PM (15 years, 1 month ago)

The only problem with that is:

In the time that the cyan spores germinate in the H.W. so will any contams. AFAIK - H2O2 will only kill ungerminated spores.

I have seen practically sterile prints made from wild specimens. This is about the only chance you would have.


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See the future...


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Invisibletripndicular
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Re: Ps. Cyan without agar work? [Re: TheShroomHermit]
    #1431571 - 04/05/03 04:22 PM (15 years, 1 month ago)

Wild prints always have a higher risk of being contaminated , so doing agar work is beneficial to issolating a "clean" isolation .
Agar work is not a scary as some may make it sound , just requires being as clean as humanly possible , patience , agar and petri dishes .
Good Luck
PS If you try agar , hravest spores from the center of the print , they are more likely to be contam free .


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Offlinecurenado
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Re: Ps. Cyan without agar work? [Re: tripndicular]
    #1432636 - 04/06/03 12:47 AM (15 years, 1 month ago)

I keep looking out for some wild. Got a buddy been ripped off twice trying to buy a sample. If one gets fresh pure wild culture one could end up with a really strong strain. Bravo!


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Yours in the Natural State!
"The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."


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Offlinecurenado
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Re: Ps. Cyan without agar work? [Re: curenado]
    #1441584 - 04/09/03 03:26 AM (15 years, 1 month ago)

Paid -
<<...the other half in a honey water tec adding h2o2 after germination if there are any impurity>>
You can add H202 to liqui-germ? I use the karo mix, but didn't know you could add H202 after germ to clean? How much?  When one of those liquid jars goes cruddy - oh are they ugly and scary looking! :shocked:


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Yours in the Natural State!
"The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."


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OfflineoO_wombat_Oo
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Re: Ps. Cyan without agar work? [Re: TheShroomHermit]
    #1441662 - 04/09/03 04:00 AM (15 years, 1 month ago)

I was actually wondering the same thing concerning some Wild Cubensis prints that I have. I don't have agar or petri and can't be f'd using it. Isn't there another way?

What are my chances if I just pretended the prints were sterile? None?

I also have a wild Copelandia Cyanescens print. Screwed if I know what I'm going to do with it though. I could't grow Copes even if the print was sterile. :smile:


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Invisibledeanofmean
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Re: Ps. Cyan without agar work? [Re: curenado]
    #1441706 - 04/09/03 04:16 AM (15 years, 1 month ago)

germinate spores on cardboard .
what i do is, soak a 1/4" square of egg carton, put it in a jar and pc it .
dab the cardboard on the spore print to pick up some spores, and put it back in the jar . after spores germinate drop it into karo water with h2o2 .
i've found when you use h2o2 in liquid culture, mycelium does'nt grow like a cloud .
instead you will see little white specks floating around, and sometimes
will look like smoke .
don't worry it is healthy mycelium .


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Amazon Shop: ½ Pint Jars, Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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