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Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Pressure Cooker, Rye Grain

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Carpal Tunnel
Registered: 04/27/01
Posts: 2,248
Awesome rye grain h2o2 tek
    #373830 - 08/17/01 09:40 PM (15 years, 2 months ago)

This is not my tek It was posted under Grain Masters by ANNO in reply to what_guy?s question about grain the tek itself was posted by major millet on the FF.

I hate rye and it hates me we have never gotten along NO matter what I tried it has always contaminated on me at some point.
Until now, after reading this tek I figured what the hell I will give it one last try. And I followed major millets friends tek, making some minor changes to suit my needs.
And low and behold it worked.
And I am happy with rye grain now (I think the rye is mad because I cheated with the h2o2 and won)
I am very happy that I was able to finally find a rye tek that worked for me and I did open air transfers, the oven tek & glove box transfers and they all succeeded and are now about to get there last shake before being mixed with 15 lbs of compost per 2 quarts of spawn.
This went over so well and smoothly that I figured why not bring it back up share with those who might not of seen it before.
If you are new or experienced with MEA and h2o2 MEA I recommend trying this tek and adding some of your own variations to suit your needs.
This would be a great tek for those of you just getting into grains and or MEA& (h2o2 mea)
I hope it will help more people that just me and MM.

Here is the link in case you want to go check out the pics that came with MM original post.

Ok, here it is folks, A detailed strain report on these great Puerto P. cubs. I will try to make my friend's tek as clear as possible, but feel free to ask questions, ill answer them the best I can.

What my friend did to kick the whole thing off was to germinate spores on 4 petris filled with MEA. As soon as germination was observed, transfers were made to peroxidated MEA.

These were allowed to colonize within about 1/8" of the edge of the pertis. Rhyzomorphic sectors were transfered to new plated of peroxidated MEA, and allowed to colonize the same as above.

The day before transfer to grain spawn, 1 cup of rye and 3/4 cup water was added to six jars. This was allowed to sit at room temperature, allowing all the nasties in the grain to germinate so they would be killed easily in the p. cooker. My friend recommends this procedure anytime your working with rye.

After soaking for 24 hours, plastic lids fitted with filter disks were put on the jars and tightend, then the jars lids were covered with foil in the usual manner. The jars were p. cooked @ 15 psi for 75 minutes. As soon as the jars could be handled they were removed from the cooker and shaken vigorously. These jars were allowed to cool overnite.

The next day the lids were removed from the jars and 5 ml of peroxide was added to each one, and the lid replaced. Again the jars were shaken vigorously. The jars were allowed to stand for about an hour before transfer of colonized agar.

After the hour to let the peroxide soak in, the agar dishes were opened and placed on the corner. My friend was not worried about contams because everything has peroxide in it. My friend took a scalpel and diced the agar, three plates worth, into small cubes, really tiny. Then he added a spoon of these cubes to each jar, replaced the lids and shook them again.

These jars were placed on top of the fridg and were totally colonized in 8 days, having been shaken once at about 30-40% colonized.

Now it was time for my friend to prepare worm castings. to be mixed with the colonized grain, making spawn that would eventually be mixed with pastuerized straw.

10 lb of worm castings were hydrated by misting until moist. Special care was taken not to turn the castings into mud. The castings were loaded into a foil turkey pan and baked in the oven for 2 hours at 155 degrees. The oven was turned off and the castings were allowed to cool overnite.

The next day the castings were removed from the oven and dumped into a medium sized rubbermaid container. The six jars of spawn were shaken to break up the grain and were dumped into the rubbermaid and mixed with the castings by hand. The lid was put on the container and it was put under my friend's bed til it was completely white.

When the worm castings/rye spawn was completely colonized it was time to pastuerize the oat hay. This is just basically straw with the grain heads still intact. A pillow case of this stuff was chopped into 3-4 inch pieces with tin snips then pastuerized in a water bath at 140-155 degrees for 2 hours. A brick wwas put on top of the pillow case to keep it submerged. After two hours the pillow case was removed and allowed to drain outside overnite.

The next day a 20 gallon clear rubbermaid, lid was clear also, was taped with duct tape, starting at the bottom and expending up 4 inches, to keep light from reaching the bulk substrate except on top. The straw was dumped into the rubbermaid, spread out by hand, then the tub od castings/rye was dumped on top of it and mixed in thoroughly. The lid was put on, and the container was covered with a sleeping bag til the substrate was completely colonized. When it was observed to be colonized, 1 bag of jiffy mix, unpastuerized, was dumped on top of the substrate and smoothed out by hand. It was misted til optimal moisture content was achieved. It was again covered with the sleeping bag til it looked like this.

Now came the fun part. The sleeping bag was removed and the container was fanned at least twice a day, more if my friend thought to do it.

There was no need to mist, as the straw contained enough moisture to keep the container
humid, since the lid was kept on except at fanning time.

Dr Bluethumbs Old School Toadstools
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Registered: 08/09/00
Posts: 1,965
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Re: Awesome rye grain h2o2 tek [Re: dimitri211]
    #373843 - 08/17/01 10:28 PM (15 years, 2 months ago)

I saw this tech over there and I've been planning on trying it, well a simplified version of it.


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Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Pressure Cooker, Rye Grain

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