stasis said:

bluespirit13 said:

I do all my agar, brf pucks and grain prep in my instant pot. No contamination.

I know it’s not recommended but I’ll gladly switch to a true PC if issues arise. Until then, I’ll take the risk for science.

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Agree, instant pot here too, great value and use for agar, grain, lc prep. just need a bit longer compared to a true PC. when I get contam is sloppy SAB technique, not the instapot.

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divineverdance said:

How are y'all differentiating good mushroom myc from other molds etc?

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It depends on what you are growing.  Cubes you can tell pretty easy when they make rhizomorphic growth.

Pans I look for leading hyphae.  Or mold spores. 

Three years working with pans and this mold still fooled me.  But it turned green two days later.



But if these ones had been mold, they already would have made a mess.

ModularMind said:

Started some Lion’s Mane on agar and dowels.

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tree frog said:

Spanish Bisporus t1.

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Just to train my eye. It looks like the top left and top right pieces has mated in the sector at 12'O clock. Or possibly two incompatible strains in the top left piece. Top and bottom ones have are not compatible on either side. And similar situation on the bottom side as in the top?

I think if I was gonna take one transfer from this plate it would be at 10-11 ish. My reason is that the growth is quite vigorous and I can clearly tell that the growth is regular and radial.

Please let me know what your thoughts on this plate is, if you have the time treefrog