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InvisiblePudinLicous
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Registered: 10/28/98
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Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond * 38
    #27090797 - 12/15/20 04:08 PM (3 years, 1 month ago)

Hi there. I am the 7th oldest active Shroomery account ('98 babbby!), but I've been laying low for a couple of decades. I've, you know, just been like, busy and stuff. But look, I have a retarded username just like you. Some things never change. I thought why not share a little with the community that has helped me over the years. I mostly grow non-actives these days, but I still have some hopefully helpful things to share.

The Instant Pot. It's a Swiss Army Knife for inexperienced folks and a great set-and-forget tool for others just wanting to do smaller runs of things (4 quarts fit at a time = 1 mono!). I like it because I can leave and do other things while it's choogling away. For people with space restrictions not looking to rock a 23q Presto, look no further. Also, it makes great food and is inconspicuous.

Should you buy a big pressure cooker? If that's what you need, then yes, but that's not what this is about. I have as many Presto's as the next person (also good luck finding one, plus they're like $140 now lol), but I find myself using the Instant Pot all the time because 1) I already have one 2) it's convenient and 3) I don't have to be there when it runs. Right now I see them online for $60 new and used ones are even cheaper. It also has some specifically great uses for agar, imo. A lot of people have had questions to their efficacy over the years, and I'm here to say: It works. This guide is for the IP-LUX 6 quart or larger size. Anything smaller will not hold quart jars.

Pasteurization
This one is my favorite. The Warm function keeps food hot after it has been cooked (obviously). The beauty of this setting is that it holds food at, you guessed it, pasteurization temp to keep it safe from bacteria for up to 10 hours. If you need to pasteurize your substrate or casing:


In case you are an idiot, it's this button.

Method 1 - using Quart Jars:
1. Follow your favorite tek for substrate prep and moisture content. FrankHorrigan has a nice one. (I use cana coir, but you do you.)
2. Load your jars without packing them too densely (it makes it difficult to get out). I use the metal jar lids upside down and don't over tighten. We're not trying to create a seal, just keep the moisture even.

3. Load them into the Instant Pot, and fill the basin with water. I go all the way up the jar. Use hot water so it heats up faster. They will start floating, but don't worry about it. Close the lid.

4. Hit the Warm button and walk away.

Kaboom. The water goes to 165ish and stays there. I run the pot and leave it until later in the day or whatever. If you are in more of a hurry, you can check the temp in the center of a jar with a kitchen thermometer. I just ran a test with 4 loaded jars at room temp with room temp water. It was at internal pasteurization temp at about 3 hours. You could also use a spawn bag, but it will probably take a bit longer.

**Just dumping wet mix into the pot will not achieve full temp at the top**

NOTE: the yogurt setting holds at 110F. It is not pasteurization!



Agar
I love the instant pot for agar prep. Use the agar recipe of your choosing. Here is how I do it.

1. Add 100 ml of cold water to your agar bottle.

2. Add 10g of Agar and stir/shake. Then add your nutrient source. I use LME (15g in this case). I then use a chopstick to breakup any clumps and mix it well.

3. Add very hot water until you are a 500ml and shake it up really well.

4. Place your filled agar bottle or bottles into the Instant Pot. I usually use two 500ml bottles. It should hold four I think. Add hot water close to the level of the top of the agar. Add some empty jars filled with water to keep the bottle from falling over.

3. Close the lid. Set the Manual timer for 20 minutes.
4. Walk away.

If the Warm function is left on, it will hold the bottles at 160 so if you need to wait a while to pour, when you are ready they will cool faster.

Give it a good shake when it is still hot. When it has cooled to pourable temp (~120F), pour the plates in front of your flow cab or in your SAB. 500ml does a 25 sleeve for me with some left over.


Let them cool until the condensation dissipates.



Bonus New fancier Instant Pots have a sous vide function. You can set the temperature to 120 and keep your hot, sterilized bottles in the bath held at the perfect ready to pour temperature (no need to buy the circulator), which is very nice. You could even set it to 185-190 and bring it right to melting temp first if it's been stored, and then swap the water and drop it down to 120.



LC
LC is basically the same as agar, but without the agar.

1. Take a jar and add 500ml of cool water.
2. Add your nutrient source. I am also using 15g of LME here. 
3. Use a chopstick or whatever and stir it up really well. Then tilt it so the unmixed chunks fall to a corner and use the chopstick to break them up until it's evenly mixed.

to this

4. Put it in the pot and add enough water that it goes to about the top of LC liquid. Don't worry, it won't fall over.

5. Set on Manual for 20 min.
6. Go do something else.


You might have some caramelized bits (which you can see here), but don't worry. The mycelium will attach to them first usually and grow out from there. It's not a problem.

Pasty's EzLC's are also great and easy to do in the instant pot. Just make them and cook on manual pressure for 20 minutes.


Grain Prep

This is for WBS.
1. Fill the pot about 2/3 full with hot water and hit the Steam setting and close the lid. Set it to Vent so it doesn't lock.

2. When the water is at a boil, SLOWLY (to prevent boil over, which I failed to do here) add two quarts of WBS and close the lid. If you want to use these to receive grain transfers, use a little less.

3. Put the lid on and set it to Seal. Hit Cancel and then start the Warm function. The time will count upward when the warming function is on.

4. At 20min, strain your WBS and let it steam off in whatever mesh/strainer you are using.

5. Load 4 quart jars evenly with the WBS, screw the lids on, and cover with foil.

For Oats:
1. Measure out roughly 1.75 quarts of oats.
2. Add to the pot and cover with hot water until it is 3/4 filled. It doesn't have to be exact, just enough water for the oats to absorb and cook in.
3. Close the lid and leave the valve on Vent. Press Steam and set the timer for 30 minutes.
4. When the time is up, strain the grain and let it steam off until dry. I have much greater success if i leave it overnight and the grain looks visibly dry on the outside.
5. Divide evenly among 4 quart jars, screw the lids on, and cover with foil.

Sterilization

Spawn
1. Load your prepped spawn jars into the Instant Pot without the rack. On mine the jars need to be arranged so the pressure valve pokes down between them for it to close. All four jars will fit with a little adjustment.
2. Fill with hot water about 1/3 up the jar. Close the lid, and set the valve to Vent.

3. Set the Manual cycle for 3 hours, use the high setting if yours has it. The Instant pot will not operate at 15psi (it can spike to that but rides more at at 242°F), so you must use a longer cycle. My jars are always fine. I have had oats, the dirtiest shit on the planet (in my experience), sit for weeks with no sign of contamination.
4. Vent the steam for 5 minutes or so after it comes to pressure and the red valve pops up.
5. Set the vent to Seal and walk away.


Voila.

After a shake.


Of course, this will work great with PF style jars as well. You should be able to get 12 jars in (with my size at least, ymmv). Use the rack underneath since there will be less water for the shorter jars. 90 minutes should work fine, but I haven't tried this personally.

Tools
1. Wrap your scalpel, tweezers, etc in foil.
2. Place them in a glass or pint jar.
3. Place the glass in the Instant Pot on the rack. Add hot water to the level of the rack.  Close the lid and set the Manual cycle for 15 minutes.

4. Once the steam starts venting, let it go for 5 minutes.
5. Seal the valve and walk away.


last, but not least

CHILI

Ingredients:
2 pounds stew meat (beef, pork, and/or lamb) I often just use one pound though
2 teaspoons oil
1 1/2 teaspoons kosher salt
1 (12-ounce) bottle of beer, preferably a medium ale
1 (16-ounce) container salsa
a few handfulls of tortilla chips crushed up (or a little masa harina instead if you have it)
2 chipotle peppers canned in adobo sauce, chopped
1 tablespoon adobo sauce (from the chipotle peppers in adobo)
1 tablespoon tomato paste
1 tablespoon chili powder (make some homemade powder like a boss and really set it off)
1 teaspoon ground cumin
1 small piece (1-2 inches of INDIAN cinnamon) not the reg grocery store shit

Directions

Place the meat in a large mixing bowl and toss with the oil and salt. Set aside.

Set the Instant Pot to saute and let it get to temp. Add the meat in 3 or 4 batches and stir to brown on all sides, approximately 2 minutes per batch. Let the pot reheat a little between batches. Once each batch is browned, place the meat in a clean large bowl.

Once all of the meat is browned, add the beer to the pot to deglaze.

Scrape the browned bits from the bottom of the pot. Add the meat back to the pressure cooker along with the salsa, tortilla chips, chipotle peppers, adobo sauce, tomato paste, chili powder, and ground cumin and stir to combine. Put the lid on and set to seal. Set to manual pressure for 25 minutes. When the pressure has released, it is ready to serve, but watch out and don't burn the fuck out of your mouth. It will be extremely hot. I fail to remember this every single time. Top with a little cheese and sour cream or whatever. Drink the other five beers.

The End. :thumbup:

p.s. get creative. You can throw anything in there. I just made a pot with leftover chicken and added pinto beans. Amazing. 


Edited by PudinLicous (11/27/23 10:04 AM)


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InvisiblefahtsterM
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond (moved) [Re: PudinLicous]
    #27099498 - 12/20/20 03:01 PM (3 years, 1 month ago)

This thread was moved from the user's journal.

Reason:
Op request


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OfflineShroomWeasel
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 1
    #27192529 - 02/07/21 07:59 PM (2 years, 11 months ago)

Thanks so much for sharing this!

I'm tip-toeing into the hobby and have been 100% in my head about what kinds of stuff I should be buying. Your post has made me feel comfortable starting with what I have.


--------------------
Our journey is an ouroboros, cultivating the inevitable decay.


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OfflineGastronomicus
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: ShroomWeasel] * 1
    #27192538 - 02/07/21 08:06 PM (2 years, 11 months ago)

I'm definitely using that chili recipe


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InvisiblePudinLicous
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: ShroomWeasel]
    #27192684 - 02/07/21 09:50 PM (2 years, 11 months ago)

Absolutely, just jump in there. It really doesn't matter as much as you'd think. You'll know when you need to upgrade things. Just get a basic grow or two under your belt so you understand the fundamentals and then you're off. :thumbup:


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InvisiblePudinLicous
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Gastronomicus]
    #27192691 - 02/07/21 09:56 PM (2 years, 11 months ago)

Get in there. I mix it up pretty much every time these days. I did it with chicken and added beans last week. It was awesome. I should change it to say let it cool enough to thicken up a little bit.. it's scorching right after the cycle and kind of thin until it cools to serving temp. Let me know how it goes :thumbup:


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OfflineOmnipositive
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous]
    #27192787 - 02/07/21 11:38 PM (2 years, 11 months ago)

I can definitely recommend it for people moving on to Agar, LC or LI. Quick and easy.


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InvisiblePudinLicous
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 2
    #27247320 - 03/10/21 08:49 PM (2 years, 10 months ago)

New and improved with photos. :tryingnottodie:


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InvisibleMunchauzen
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 1
    #27247364 - 03/10/21 09:45 PM (2 years, 10 months ago)

interesting, thanks for sharing!


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OfflineMatty_Rulez
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 2
    #27247718 - 03/11/21 06:57 AM (2 years, 10 months ago)

This is great!  OG Chili recipe.  :grin:

I've had a few people ask me if the instant pot is good for these things...  thanks for the great write-up.

Cheers!  Welcome back!


--------------------
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Offlinerockyfungus
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Matty_Rulez] * 1
    #27247723 - 03/11/21 07:01 AM (2 years, 10 months ago)

Instant pot is a great starter pressure cooker. Especially if you already have one. I would use a PC over the rice cooker, but I started with an instant pot.

I've squeezed bags into it, but wouldn't recommend it.

I only use an instant pot for small batches of agar, LC and sterilizing tools.


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OfflineDharmaForKarma
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: rockyfungus] * 1
    #27248042 - 03/11/21 11:11 AM (2 years, 10 months ago)

At last I can come clean about my secret shame:  I love the instant pot.  It definitely works, I’ve kept jars for 6 months with no contam.

Fwiw, I run oat sterilization for 2 hrs 30 minutes. Came to that after a bunch of experimenting.


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InvisiblePudinLicous
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: DharmaForKarma]
    #27248722 - 03/11/21 06:25 PM (2 years, 10 months ago)

Thanks everyone. I appreciate it. I'm taking pics for LC also and will add that on eventually.

No shame in this game, Dharma. If I can turn something on and leave it alone while I can go do whatever else, I'll use it whenever possible. I  now use it for agar, tools, and LC 100% of the time. Also, I was using about the same time for oats (and wbs) also, but figured why not a little bit longer. No harm, and I've had lots of contam issues with oats historically.

Rocky, glad to hear other people are rocking the pot :thumbup:


Edited by PudinLicous (03/11/21 06:31 PM)


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OfflinecronicrFacebook
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 1
    #27248827 - 03/11/21 07:12 PM (2 years, 10 months ago)

i keep a couple in my room for lc and heating agar ect


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InvisibleWall.E
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: cronicr] * 1
    #27249076 - 03/11/21 10:02 PM (2 years, 10 months ago)

Where's my man aka? This is his jam

I use mine for small batches of agar and pasteurizing.


--------------------
Life’s shit, but I’m loving it



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InvisiblePudinLicous
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Wall.E]
    #27251675 - 03/13/21 06:14 PM (2 years, 10 months ago)

Added LC for folks that need help figuring it out. Now I have to figure out what to do with all this LC, haha. Maybe some gigantic bags of ATL7 with this awesome sclerotia producing culture I have. :thumbup:


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 1
    #27265732 - 03/23/21 09:02 AM (2 years, 10 months ago)

really appreciate this write up! will give the chili recipe a shot and report back. im going to try some rice with it!


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Offlinenonamekevin
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: spicandspawn] * 1
    #27265784 - 03/23/21 09:50 AM (2 years, 10 months ago)

I've got the 6qt duo gourmet, and I can only fit 3 Kerr quart jars in at one time. FYI for those who might have the same model.

Pudin, you put the jars in to the instant pot without lids or a trivet at the bottom? No issues with burning the grain? And so I understand, you start the pc with the vent open, then close the vent after 5min of it being at pressure?


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InvisiblePudinLicous
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: nonamekevin]
    #27265838 - 03/23/21 10:30 AM (2 years, 10 months ago)

spicandspawn, sounds good. the rice will thicken it up a lot. add some extra spice too :smile:

Nonamekevin, nothing on the bottom. I've never had an issue. The Instant Pot is designed to not scorch food, and I've never had burst or burnt grains. And that is correct on the vent. :thumbup:


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Invisibleseldom seen
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 1
    #27265852 - 03/23/21 10:42 AM (2 years, 10 months ago)

Fuck yeah, nice write up.  I love this place.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: nonamekevin]
    #27267052 - 03/24/21 07:29 AM (2 years, 9 months ago)

nonamekevin,

I always use the trivet that comes with the IP. Lids would work too.

Contrary opinion: I don't leave the vent open.  The red popper takes care of releasing air as steam builds and pressure rises.  I close the lid, turn it on, walk away.


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InvisibleWall.E
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: DharmaForKarma]
    #27267082 - 03/24/21 07:51 AM (2 years, 9 months ago)

I think i read somewhere that part of venting is to allow air to escape from your vessels to increase your odds of success. Bringing it to pressure too quickly might allow the air inside the pc to escape, but not from the bottom of your jars.


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InvisiblePudinLicous
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Wall.E]
    #27267209 - 03/24/21 09:51 AM (2 years, 9 months ago)

On my model it is physically impossible to close the lid with anything under the jars, but it you can that's cool. It doesn't make any difference though. I've never had any issue in countless batches, but in the presto it's necessary of course.

For the venting, if it works for you that's cool too. In my opinion, growing is all about what you can get away with. :thumbup:


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Wall.E] * 1
    #27267211 - 03/24/21 09:53 AM (2 years, 9 months ago)

This thread inspired me to give it a try yesterday.

I pc'd a 30oz container of yellow popcorn on high pressure for 45min. Water was about an inch above the corn. It probably could have gone for an hour, as it didn't feel as tender as when using a presto pc for 30min. Dried the corn on a towel on the counter until no moisture was observed on a paper towel, and filled 3qt jars aittle less than 2/3 full. Unmodified lids flipped over and cracked open a 1/4 turn before getting wrapped in foil and placed in the instant pot.

I filled the instant pot with water so that a little more than 1/3 of the jar was in the water. I do kind of like the idea of leaving the vent open and waiting for steam to come out, not for any other reason than most times, my instant pot won't pressurize on its own without me having to push down on the lid to get it to seal. I noticed yesterday that by leaving the vent open, my pressure indicator was already popped up, so I just had to close the vent and I was rocking.

Also, I misread the time for the grain, so I went 2.5 hours instead of the 3hrs recommended. Let the pressure naturally reduce and removed the jars.

Im not ready to inoculate anytime soon, so this may be a good thing to have the jars sit and see if any contam shows up. Foil is kept on, lids are still cracked open 1/4 turn. Hopefully 2.5hrs was good enough.

:rockon:


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Invisiblesponglord69
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: nonamekevin] * 1
    #27267221 - 03/24/21 10:06 AM (2 years, 9 months ago)

Brilliant tek. Thank you for sharing. Is it safe to put quart jars in like that? The pressure cooker line indicator is lower than the top of the jars.


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InvisiblePudinLicous
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: nonamekevin]
    #27267235 - 03/24/21 10:15 AM (2 years, 9 months ago)

Kevin that's great!! The 2.5 hours is probably fine. You will find out before long if it isn't. Keep me updated.

Thanks Sponglord, I hope you find it useful. That line is for pressure cooking things like soup. You don't want to go above the line in that case, but jars it's fine and totally safe. Also when sterilizing the jars the water is not above that line. :thumbup:


Edited by PudinLicous (03/27/21 12:16 AM)


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Invisiblesponglord69
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 1
    #27271060 - 03/26/21 11:01 PM (2 years, 9 months ago)

That's really great to know Pudin, thank you. Will have to give this a shot. Can't beat a set and forget tek.


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Offlinebuellia
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: sponglord69]
    #27271087 - 03/26/21 11:51 PM (2 years, 9 months ago)

Anyone have any experience using the Crock-Pot knock-off of the Instant Pot for this? Best I can find is that it maxes out around 9ish PSI.


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InvisiblePudinLicous
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: buellia]
    #27271102 - 03/27/21 12:15 AM (2 years, 9 months ago)

It will work, but you might need a longer cook time. The instant pot runs at 10-11 psi basically. The 3 hours is a little extra long though, you can try 3-3.5 hours. Let the jars sit for a week at least if you want to test them. More likely two.


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Invisiblesponglord69
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous]
    #27277573 - 04/21/21 11:48 PM (2 years, 9 months ago)

Can't wait to give this a go. Couldn't access this page over the past month or so :frown:.

Just to be sure , when you are making grain jars,  you don't use the trivet? do you place any jar rings or anything under the jars at all?


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: sponglord69]
    #27280312 - 04/24/21 10:50 AM (2 years, 8 months ago)

I read somewhere if your lid won't close using the trivet place a folded dishtowel (or anything like it) on the bottom.


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Offlinesimplegood78
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Threads2theSoul] * 3
    #27439246 - 08/23/21 07:20 AM (2 years, 5 months ago)
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Adding a placeholder to outside verification that the Instant Pot can sterilize similar to an autoclave.  I added it here, because I come back to this post so much.

Swenson, Vaille Alena, "Assessment and verification of commercially available pressure cookers for laboratory sterilization" (2019). Masters Theses & Doctoral Dissertations. 331.
https://scholar.dsu.edu/theses/331

Dakota State University student Vaille Swenson recently conducted research upon the Instant Pot and came up with the conclusion that the Instant Pot is quite effective at sterilizing all bacteria including the toughest survivors of the lot.

Minimal Time to sterilize
  • Nutrient Agar: 15 minutes
  • Soil/Dirt Water: 45 minutes
  • Dissection scissors: 30 minutes


It took 150 minutes to inactivate the Geobacillus stearothermophilus spore.


--------------------
Tek: Uncle Ben's, Agar, PF
Equip: Instant Pot, Box Fan, Blendtec
Level: Knoobie learning


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OfflineMeatball
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 1
    #27475316 - 09/20/21 08:11 AM (2 years, 4 months ago)

I've done agar in mine a few years ago and it did great. Getting back into things so I was glad to find your post to give me all the details again to run it. And the keep warm function is brilliant...didn't think of that. Thanks again!


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OfflineThe Tao
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Meatball] * 1
    #27475558 - 09/20/21 11:29 AM (2 years, 4 months ago)

I have a 6 quart instant pot. Perfect for wide mouth 1/2 pint BRF jars. Water to the height of the trivet. 4 jars on the bottom, 3 on top. Top jars adjusted to avoid the release valve. 120 minutes on high pressure with the stay warm function off. I do not vent, just set and forget.
Also does spareribs in under 20 minutes once it’s up to pressure.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: simplegood78]
    #27555322 - 11/23/21 04:51 PM (2 years, 2 months ago)

I have been gone for a while because I lost my lab in a flood, so I am just seeing this, but thank you! That's amazing. Pretty cool that it lines up with the 2.5 hour time that people have discovered through trial and error. Thanks for sharing!


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Offlineschpat
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 1
    #27555997 - 11/24/21 07:29 AM (2 years, 2 months ago)

This is great info, I really like the links to the articles.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: schpat] * 1
    #27558455 - 11/26/21 01:21 PM (2 years, 2 months ago)

For me grain goes in my 75x, Litterally everything else goes in the IP. Agar pour and no pour, lc, water for li, syringes, tools, casing, Litterally everything. Having stuff more or less automated makes life much easier.


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Offlinerockyfungus
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Drboomer] * 1
    #27559952 - 11/27/21 07:29 PM (2 years, 1 month ago)

Instant pot is the tits. I made a roast the other day, perfection.

Next day few tools, baby jars of grain, lc, and syringes. Vent for 10ish mins close the vent and FORGET IT!!!!!!


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InvisibleaNewBeginning

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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 1
    #27573891 - 12/08/21 05:53 PM (2 years, 1 month ago)

Quote:

PudinLicous said:
I have been gone for a while because I lost my lab in a flood, so I am just seeing this, but thank you! That's amazing. Pretty cool that it lines up with the 2.5 hour time that people have discovered through trial and error. Thanks for sharing!




Sorry to hear about your lab. Are you working on the rebuild?

Thank you for this great write up.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: aNewBeginning] * 1
    #27574075 - 12/08/21 08:52 PM (2 years, 1 month ago)

Great tek! :mushroom2:

I'll try to use my InstaPot Duo for agar. Very convenient for small jobs.

For agar, I'm currently doing 20 minutes@15PSI in Presto. Are 20 minutes enough for agar in InstaPot?

There is a newer InstaPot Max model which can achieve 15 psi.


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InvisibleDrboomer
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: fungusul] * 1
    #27574196 - 12/08/21 10:46 PM (2 years, 1 month ago)

It peaks at 15 psi, it nominally runs at 11-13 psi. I do 45 mins for agar. 30 gave me no real issues but better to side with caution as the only technical paper I could find said 45.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: fungusul]
    #27583731 - 12/16/21 01:28 PM (2 years, 1 month ago)

I always do 20 minutes for agar in the instant pot and never have contam issues. To each their own! 40 isn't going to hurt


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: aNewBeginning]
    #27583733 - 12/16/21 01:32 PM (2 years, 1 month ago)

Quote:

aNewBeginning said:
Sorry to hear about your lab. Are you working on the rebuild?

Thank you for this great write up.




my pleasure, I'm glad you like it.

Yep, it sucked pretty bad, but it was an opportunity to take a break and start fresh. I'm just now getting back up and running in a minimal setup.


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OfflineMushyMom
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 1
    #27609012 - 01/06/22 11:19 AM (2 years, 21 days ago)

This is great.  I have my giant pc but this would help when I’m trying to make an inconspicuous batch of agar


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OfflineBlueAndOrange
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: MushyMom]
    #27754832 - 04/28/22 09:48 AM (1 year, 8 months ago)

I’m trying this today. I have an 8 quart IP Ultra, and I just ran the cycle on 4 bottles of agar. Going to fill 50 plates today for a bunch of upcoming experiments.

The cool thing about the ultra is you can set a custom keep warm temp, so after my Instant Pot cycle was up, I set the keep warm temp to 120 so I’ll have enough time to pour all the plates while the agar is cool enough to minimize condensation, but no risk of it getting to cold to keep pouring. I’ll report back on how well this works.


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OfflineBlueAndOrange
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: BlueAndOrange]
    #27755496 - 04/28/22 06:34 PM (1 year, 8 months ago)

So my review:

I did the IP sterilization and set my warm temp to 120. Ran it and let it sit and wait until I was ready to pour. Poured 50 plates and all 4 bottles were 118 degrees when I grabbed them. Seemed to work great. Got more condensation than I was hoping for, but that’s because I pour too thick, not the IP TEK. Hopefully these are all sterile and ready to go!


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: BlueAndOrange]
    #27755551 - 04/28/22 07:19 PM (1 year, 8 months ago)

Used this tek several times now with popcorn, 2.5hrs on high pressure, followed by NR overnight, cause I'm not in a rush.

I typically let my jars sit for a week or so before nocking up, to see if any nasties grow, and I'm not in a rush. Haven't had an issue yet.

I do need to try this tek for agar, since I'd like to dip my toes in agar work.


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OfflineBlueAndOrange
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: nonamekevin]
    #27755555 - 04/28/22 07:22 PM (1 year, 8 months ago)

Agar is the most fun part of the work imo. And the IP version was way more pleasant than running a PC.


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InvisibleRisingTide
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous]
    #27763825 - 05/05/22 01:26 AM (1 year, 8 months ago)

I upgraded to a used instant pot max just before I found this tek, mostly for the 15psi.  After reading, I decided to resell it and stick with the 8qt ip duo.  I do jars infrequently so a 3 hour run overnight is just fine.

I had the ip duo long before I used it for grain jars.  It was running 3 qt jars fine with the trivet before I read this. Trying out 4 jars tonight with a towel in the bottom.  Always happy to use what I have and not accumulate more gear.  And getting more and better use out of it now too.

Thank you!


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Invisiblekreg
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: RisingTide]
    #27763827 - 05/05/22 01:28 AM (1 year, 8 months ago)

this is hot, thank you


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OfflineKigz
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: kreg]
    #27772667 - 05/11/22 01:22 PM (1 year, 8 months ago)



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OfflineBlueAndOrange
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Kigz]
    #27772694 - 05/11/22 01:45 PM (1 year, 8 months ago)

Quote:

Kigz said:
Will this also work?

https://www.amazon.com/Instant-Pot-Multi-Use-Programmable-Pressure/dp/B00FLYWNYQ




Spend the extra $9 and get the 8 quart. I have both 6 and 8 quart instant pots and my media bottles fit better in the 8.

But yes, that duo should do the trick. The ultra is a little nicer since it has the customizable keep warm temp to keep your agar bottles at pouring temp longer.


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OfflineKigz
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: BlueAndOrange]
    #27772700 - 05/11/22 01:55 PM (1 year, 8 months ago)

Can't find the 8 quart model on the Swedish  market. Only 6. Will it be to tight for jars (15cm hight) and plastic bags?


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OfflineTight Lunchbox
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Kigz]
    #27772729 - 05/11/22 02:22 PM (1 year, 8 months ago)

These things are not ideal for grain, but for agar they work great.


--------------------
"it's all a joke between mom contractions and coffin fittings"


The most useful tool for noobs


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OfflineHuskies
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Tight Lunchbox]
    #27799803 - 05/31/22 05:01 AM (1 year, 7 months ago)

Questions :

1- Even though it's steaming, the timer for the Steam cycle never turned on, any ideas why? 8 quart just like yours

2- you say 20 mins for agar: does that include pasty plates? does the extra surface/air/containers mean it should stay longer? ive been doing 45ish. I just need to find a straight bottom cause my steam rack I use there leaves me with some slanted plates.

3- Would you say hyour success has been consistent with grains? Doing oats today because I cant figure out where the fuck my weight for my Presto went :frown:


--------------------
I call them Huskies cause you tell them to go "Mush! Mush""


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Invisiblemyc_ousin_vinny
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Huskies]
    #27799826 - 05/31/22 05:35 AM (1 year, 7 months ago)

Old school! :respect:


--------------------
Bod's Easy AF Search Engine TEK

Let's stay focused on what works consistently.

"Sitting in a bunker here behind my wall..."


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: myc_ousin_vinny]
    #27799843 - 05/31/22 05:58 AM (1 year, 7 months ago)

I wish there were more programmable PSI pressure cookers. set 15 psi, 90 minutes, etc.


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Offlinegun fuy
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: BlueAndOrange]
    #28031305 - 11/03/22 03:08 PM (1 year, 2 months ago)

Interesting thread!

Currently hesitating between getting one of these two which are available on the market where I live
- 10L PC Tefal "Eco minute"
- 7.5L Ninja Foodi 9-in-1 (model OP500EU).

The tefal one can cook at 117 C (so about 13 PSI). Clearly indicated in the spec sheet.

Can't find any such specs about the Ninja except when asking their customer service they said that it runs between 50 and 120 kPa - which is pretty vague. I'm guessing it peaks at 120 and runs a fair bit lower. There is a HI and LO setting and they couldn't even narrow down the ranges for those two settings.

I was leaning toward the PC. It has a couple of advantages apart from actually knowing the temp and pressure.
- Less bulky (for it's volume)
- More volume
- I think dishwasher safe
- Cheaper
- Doesn't take up prime real estate on a bench top


Now though, after skimming this thread, I'm less sure.
Frustrating :P


--------------------
"Most multispore projects will be more productive than most isolates. The differences is a FEW isolates will be killer, and those are the ones you want to save." - RR


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: gun fuy]
    #28031311 - 11/03/22 03:11 PM (1 year, 2 months ago)

Id be concerned that the eco minute couldnt hold enough water for 1.5-3 hour sterilization runs at those temperatures.

Why cant you hold out for something bigger?


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: cooleko] * 1
    #28031360 - 11/03/22 03:41 PM (1 year, 2 months ago)

Dude I use an Intantpot, because I've got one.  At my scale it's great.  But experiments have shown that other brands of electric pressure cooker do not perform as well.

I'll upgrade to a proper PC soon though


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Offlinegun fuy
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: cooleko]
    #28032083 - 11/04/22 02:43 AM (1 year, 2 months ago)

Quote:

cooleko said:
Id be concerned that the eco minute couldnt hold enough water for 1.5-3 hour sterilization runs at those temperatures.

Why cant you hold out for something bigger?






First I've heard of that being a problem, and plenty of others seem to have smaller PCs. Perhaps they're all 15 PSI ones?
But it's a worthwhile point to investigate, thanks.

I suppose the multicookers like IP and Foodi release less steam so that doesn't become a problem?

Edit - replying to the question in your reply
Don't want to get something bigger than that mainly for space and storage limitations in the appartement. That and I'm just starting out and don't plan on doing huge batches. I doubt that would become a thing but if it does it wont be for a few years and at that point I can invest.
Edit2 - add the quote of what i'm replying to


--------------------
"Most multispore projects will be more productive than most isolates. The differences is a FEW isolates will be killer, and those are the ones you want to save." - RR


Edited by gun fuy (11/08/22 04:42 AM)


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Offline4-HO-Gumbo
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: gun fuy]
    #28032111 - 11/04/22 03:22 AM (1 year, 2 months ago)

Here’s a study from 2018 assessing the ability of commercially available electric pressure cookers to neutralize autoclave test ampoules with Geobacillus Stearothermophilus spores, a thermophilic (clearly) bacteria

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289433/

Only the instant pot was able to kill the spores
The test was done with the ampoule submerged in the water though, so it doesn’t directly correlate to mostly hydrated grains, but is scientific proof that it gets hot enough.
This wasn’t even the newest, best instant pot, just the ones capable of 12.x psi

“These results suggest that, depending on the nature of the work undertaken, store-bought pressure cookers can be an appropriate substitute for commercial autoclaves”


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Edited by 4-HO-Gumbo (11/04/22 06:42 AM)


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: 4-HO-Gumbo]
    #28032157 - 11/04/22 04:35 AM (1 year, 2 months ago)

Even P9 will tell you that Instantpots will work to grow mushrooms, but they are not the optimal tool.  I totally get that and agree with him. You can still get good results for grain to bulk.

I've found that using crack's no prep millet in 1 quart jars for 180 min works really well.  Probably something to do with the small size of the grain.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: schpat]
    #28089373 - 12/08/22 08:29 PM (1 year, 1 month ago)

I've been using my instant pot for a couple years just for master grain jars and sometimes agar or LC, but never tried pasteurization til recently. I got trich right when my pan cyans finished colonizing the bulk, but I also probably messed up because the OP says it took 3 hours TO GET to the right temperature all the way through, but I put it on warm for just 3 hours. So does that mean I should do 4-5 hours? I typically just do the bucket tek for coir but figured this would be good for just a small amount of horse manure.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Eminence]
    #28089411 - 12/08/22 08:41 PM (1 year, 1 month ago)

Quote:

Eminence said:
I've been using my instant pot for a couple years just for master grain jars and sometimes agar or LC, but never tried pasteurization til recently. I got trich right when my pan cyans finished colonizing the bulk, but I also probably messed up because the OP says it took 3 hours TO GET to the right temperature all the way through, but I put it on warm for just 3 hours. So does that mean I should do 4-5 hours? I typically just do the bucket tek for coir but figured this would be good for just a small amount of horse manure.




in the op he sets it to Warm for 3 hours and that gets the internal temp to 165. If you like, add another half hour to test your theory of insufficient pasteurization. But that should be plenty of time. You could also pressure cook it but I’ve never tried that with manure.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: DharmaForKarma] * 1
    #28091095 - 12/09/22 10:02 PM (1 year, 1 month ago)

Awesome tek


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: DharmaForKarma]
    #28092780 - 12/11/22 02:01 AM (1 year, 1 month ago)

I know I'm saying if it takes 3 hours to get to the right temp, that isn't being clear that that is how long it takes to actually finish pasteurizing at that temp. I was just trying to be careful about adding time because I have read TC's say pasteurizing too long starts to kill off the good bacteria that helps keep it contaminant free. But then again I feel like when I do a bucket tek with coir, the boiling water stays at or above pasteurizing temps longer than it takes to pasteurize fully too, but I'm more concerned about manure contaminating than coir.


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Invisiblecooleko
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Eminence]
    #28093175 - 12/11/22 10:34 AM (1 year, 1 month ago)

Coir doesnt have 'good bacteria' necessary to balance it out, it is expected to be completely inert. Manure needs the healthy balance of good bacteria to keep the system healthy and stable. That is why your coir can stay above 165 for longer than manure.


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Offlinealexmorgan114
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: cooleko]
    #28096679 - 12/13/22 11:02 PM (1 year, 1 month ago)

Thank you!!


--------------------
:mushroom2:


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InvisiblePsillySeeEms
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: alexmorgan114]
    #28097158 - 12/14/22 11:47 AM (1 year, 1 month ago)

What would you guys say the best no pour agar containers for 6qt instant pot would be?

PP5 plastic ones? What size, and how many fit? Modded, non modded? Any recommendations?

I was looking at 6 packs of twist and lock brand from target, for about $3, but the are on the larger size..

I can't find a deal on non quilted smooth 4 oz ball jars, and willing to settle on the quilted ones if need be. Can't fit as many in the IP though.. What would be the best lids if I do get jars? Is it possible to make see through lids with clear pp plastic cut into circles, with the original rings?

I have old baby food jars, and could clean them up, fitting quite a few. Would I wanna cook with the lid loose, or drill a hole with MP tape, and cover with foil when cooking?

It would be nice to fit multiple smaller 2oz size plastic cups of some kind, which I'm guessing would fit way more per batch...

Wondering if there is a specific kind that are the best for the 6qt IP..

Thanks!


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: cooleko]
    #28113644 - 12/27/22 05:49 AM (1 year, 1 month ago)

Well either way, I got trich again after putting some horse manure in the jars on warm for 6 hours. I only ever get trich with pan cyans, but it's also the only thing I really use manure for. I'm just gonna say fuck it and not even use manure I guess


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 1
    #28151491 - 01/22/23 04:11 AM (1 year, 6 days ago)

Do you have the 6 or 8 quart instant pot? I have a six quart and it was impossible to fit more than two wide mouth quart jars using the grey plastic lids.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Floret]
    #28156340 - 01/24/23 10:37 PM (1 year, 3 days ago)

I got the 6 qt.

To answer my question above, I found that I can almost fit 15 of the Target brand twist & store containers. 14 of them fits perfect (with a little wiggle room) all stacked around on on the trivet, to give clearance for the vent protector under the lid.



I also found that the containers fit just right inside bush's baked bean cans, being as wide as they are. I was thinking I would use them like sleeves,just as long as I can fit 5 sleeves/cans in the pot side by side. Not sure on that yet, I'll have to get more baked beans first. I'll bend a can to make them all fit if needed, and use the shorter bush's bean can on the double stack. :thumbup:

The cans should be fine to pressure cook, correct? Think it would help, or better to just layer or wrap with foil?






Also, 5 of the smaller 16 oz pint jars fit like a glove:




Suppose I could safely sterilize just about any kind of grains inside those small jars in the IP, being as small as they are, right?


Edited by PsillySeeEms (01/24/23 10:45 PM)


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PsillySeeEms]
    #28164780 - 01/30/23 05:46 PM (11 months, 21 days ago)

Thanks, looks snug in there. Guess I need to switch over to pints.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PsillySeeEms] * 1
    #28164789 - 01/30/23 05:49 PM (11 months, 21 days ago)

Quote:

PsillySeeEms said:
What would you guys say the best no pour agar containers for 6qt instant pot would be?




I fit a 20 pack of holy grail no pours in my 6qt


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InvisiblePsillySeeEms
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Floret]
    #28164866 - 01/30/23 06:37 PM (11 months, 21 days ago)

I did end up reading through the entire holy grail container thread, but went ahead and snagged the twist and store containers anyway, because they were like $2.50 a 6 pack, and conveniently at the store. They are the most durable looking of all the pp5 containers I could find off the shelf, way better than zip lock brand that cost even more for a 4 pack.

I had a pack of those HG's in my shopping cart too, but heard the lids might warp.. Not that is seems to matter though.. What brand did you choose? 

It does look like a 20 pack would drop right down in the 5 pint jars perfectly in stacks of 5.

I'll have to switch over soon as I get some experience in the ones I got


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PsillySeeEms] * 1
    #28167969 - 02/01/23 06:34 PM (11 months, 19 days ago)

Prob got the same ones you have in your cart. From what I read, the lids warp if the seals are too tight, whether from not untwisting the 1/4" or stacking them too high.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: schpat]
    #28167991 - 02/01/23 06:45 PM (11 months, 19 days ago)

YES! I love mine for all my mushroom sterilization and pasteurizing! Super easy and reliable!


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: tbone8352]
    #28182071 - 02/11/23 09:56 AM (11 months, 9 days ago)

Pudin seems to be using an 8qt in his writeup. I have the exact same media bottles and they don't fit upright in my 6qt Instant Pot. Time to look at Craigslist for an 8 quart model ahaha. I have a proper PC but it's inconvenient for making small batches of agar. Instant Pot is set and forget.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous]
    #28188174 - 02/15/23 07:04 AM (11 months, 5 days ago)

I‘m trying to rule out some sterilization issues using the Ninja OM500/Foodie Max (might be similar to the ip).

I did a round 2:45h with 0.5l water, letting it vent until the pot closed the seal (swimmer) after around 20min. When opening there was only a little bit of water left in the folded towel i use as a rack. During the sterilization no steam was released.
Could this mean that there is insufficient water in the pot to produce sufficient steam i.e. use more water to begin with?

Pudin wrote no rack needed.  If i had the jar standing straight in the water would it boil the grain or rather prevent the steam from reaching the grain on the bottom of the jar?


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: nXr] * 1
    #28197050 - 02/21/23 07:32 AM (10 months, 30 days ago)

Heads up - Instant Pot Duo 8 quart on sale for $74 at Amazon. This one holds four quart jars on the trivet. I’ve used mine for years with 100% success. No green jars due to incomplete sterilization, ever. 

For someone who does not need the higher capacity of a Presto or AA, this is the bomb. I also cook food with it - it’s excellent at that too.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: DharmaForKarma]
    #28197096 - 02/21/23 08:13 AM (10 months, 30 days ago)

How long do you run your grain cycles?


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: dowodenum]
    #28197145 - 02/21/23 08:56 AM (10 months, 30 days ago)

Quote:

dowodenum said:
How long do you run your grain cycles?




Vent 10 minutes then 2:45 at max pressure. I’ve worked mainly with oats, just starting with wbs and millet. So far similar timing seems to work. Fwiw I can easily run 3 batches in 24 hours. That’s 12 jars / 3 tubs with just a few minutes attention at the beginning and end.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: DharmaForKarma] * 2
    #28197159 - 02/21/23 09:09 AM (10 months, 30 days ago)

I snagged one of these recently for agar/LC



Made for canning so it hits 15psi, and even has a built in vent timer cycle

can be set manually for much longer than my off brand 13 qt (gowise) IP. Gonna keep that one for pasteurization because its so big and damn convenient but hooing these electic “canners” get made more and bigger like IP’s did. Presto made one for s little but it got discontinued..


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: AyePlus]
    #28198599 - 02/22/23 03:23 AM (10 months, 30 days ago)

I keep waiting to find an 8qt, and passing up practically brand new 6qt duo and lux models at goodwill and value village left and right.. besides the first one I got that was mint. About to say heck with it, and buy 2 more 6 qts. I'll need roughly 30 amps to run 3 at once, lol. 15 small mouth pint jars at once isn't so bad, set and forget, right?

On Sundays, they have 50% off at goodwill, so you can get one for $20 usually, if its the right tag color. Always 2-3 of them, at any of the second hand stores you go to around here. Some you can tell were never used once, because the sealing ring doesn't smell at all, and not a scratch in the pot. Even have the protective plastic wrap on the buttons still.

People selling used IPs online all want like 50 for one they used the heck out of for the last 5 years.. ya no thanks. Brand new ones are 60-90. I'll take 3 for $60 from the second hand stores myself :wink:

How about 5? Would you rather have a 23qt presto, or 5 IPs with built in timers?


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PsillySeeEms]
    #28198601 - 02/22/23 03:35 AM (10 months, 30 days ago)

The delay start feature would come in real handy if trying to run multiple sets of IPs on your countertop, without tripping the breakers. Set them to go off in pairs, so your not pulling more than a few thousand watts at any time. Come home from work and have a fleet of like 6-8 pots full of jars good to go. IPs are a dime a dozen now, and replacement parts are cheap. Haha


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PsillySeeEms]
    #28203352 - 02/24/23 11:26 PM (10 months, 27 days ago)

Does anyone vent their instant pots for agar? OP doesn't mention it, but most people who use actual pressure cookers do. I find it odd that he vents for grain but not for agar. Anyone know the reasoning?


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Way]
    #28203382 - 02/25/23 12:20 AM (10 months, 27 days ago)

Always vent. The only thing I use my instapot for in this hobby is agar and I vent for 15 minutes.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: JW123]
    #28203463 - 02/25/23 03:19 AM (10 months, 27 days ago)

Quote:

JW123 said:
Always vent. The only thing I use my instapot for in this hobby is agar and I vent for 15 minutes.




Do you mind if I ask how long you PC for? I feel iffy about PCing agar for 20 minutes at 12 PSI when that is the minimum recommended for 15 PSI.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Way]
    #28203465 - 02/25/23 03:30 AM (10 months, 27 days ago)

I set it for 45 and vent for 15 of that.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: JW123]
    #28203469 - 02/25/23 03:59 AM (10 months, 27 days ago)

Quote:

JW123 said:
I set it for 45 and vent for 15 of that.




Hey thanks, I'll give that a shot and see how it works!


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous]
    #28203495 - 02/25/23 05:15 AM (10 months, 27 days ago)

I have been waiting on my pressure cooker but weather has affected it's delivery. I have one of these and this will help me get started while I wait for the pressure cooker. Thank you for the awesome recipe at the end


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: JW123] * 2
    #28203617 - 02/25/23 07:48 AM (10 months, 26 days ago)

Quote:

JW123 said:
Always vent. The only thing I use my instapot for in this hobby is agar and I vent for 15 minutes.




Fwiw - both methods work.

I do not do any additional venting for no-pour agar or LC.  The spaces within the plates of agar and jars of broth are far less insulated and far more conductive to heat than the centers of quart jars loaded with grain. Additionally, the IP’s internal volume is tiny compared to a Presto or AA canner. Dead air spaces just aren’t a thing in this situation.

I typically cook agar and LC for 25 minutes and have had 100% success with sterilization over years of IP use.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: DharmaForKarma] * 1
    #28204851 - 02/26/23 01:01 AM (10 months, 26 days ago)

Quote:

DharmaForKarma said:
Quote:

JW123 said:
Always vent. The only thing I use my instapot for in this hobby is agar and I vent for 15 minutes.




Fwiw - both methods work.

I do not do any additional venting for no-pour agar or LC.  The spaces within the plates of agar and jars of broth are far less insulated and far more conductive to heat than the centers of quart jars loaded with grain. Additionally, the IP’s internal volume is tiny compared to a Presto or AA canner. Dead air spaces just aren’t a thing in this situation.

I typically cook agar and LC for 25 minutes and have had 100% success with sterilization over years of IP use.




The only reason I had asked was because I have had good luck so far with doing 25 minutes for my single media bottles with no vent. The last cycle I did two 500ml bottles and almost all my plates were contammed out, with no change in my technique. I could only assume they didn't get fully sterilized. Hoping 45 minutes + vent works better this time.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Way] * 1
    #28205803 - 02/26/23 04:57 PM (10 months, 25 days ago)

This is exactly what I needed. Thanks Pudin!:cool:


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PsillySeeEms]
    #28213480 - 03/04/23 01:58 AM (10 months, 20 days ago)

Check offer up. For $50 I just scored a brand new insta pot pot as well as the instapot vision pro air fryer with works great as a dehydrator.

Would normally cost about $250 from Amazon. But they sell a lof of open/damaged box return items on offerup. Sure, the items may come with a couple dings but so what?

If your in a major metropolitan area your likely to find dozens of resellers. Just verify the item has been tested and has all parts.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous]
    #28226189 - 03/12/23 10:11 AM (10 months, 11 days ago)

I needed an electric pressure cooker because I couldn't use my parent's stove. I bought an Instant pot for like 70 dollars after tax and it actually ended up being a minor expense compared to the total of all the other supplies. I spent 10 dollars on a 56qt clear tub from Walmart if that gives you an idea of how much more expensive things are in 2023.


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Offlineomgflyingbanana
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: 45cal] * 2
    #28231872 - 03/16/23 11:00 AM (10 months, 7 days ago)

Quote:

45cal said:
Check offer up. For $50 I just scored a brand new insta pot pot as well as the instapot vision pro air fryer with works great as a dehydrator.

Would normally cost about $250 from Amazon. But they sell a lof of open/damaged box return items on offerup. Sure, the items may come with a couple dings but so what?

If your in a major metropolitan area your likely to find dozens of resellers. Just verify the item has been tested and has all parts.




I live in a city and was able to find an 8 quart Instant Pot on fb marketplace for $20. Sure it was secondhand but can't beat the price.


Edited by omgflyingbanana (03/16/23 11:01 AM)


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Offlinepsilocyboss
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: omgflyingbanana]
    #28239145 - 03/21/23 05:52 AM (10 months, 3 days ago)

what happens if you forget to let it vent at the start? I thought i had it set to vent but I didn't and when i did, it only let air out for about 1.5mins. I did a 3 hour PC


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: psilocyboss] * 1
    #28239151 - 03/21/23 05:58 AM (10 months, 3 days ago)

Potentially didn’t transfer heat well enough to the middle of your bags/jars. Steam transfers heat better. That’s the gist I believe.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: psilocyboss] * 2
    #28239311 - 03/21/23 08:54 AM (10 months, 2 days ago)

Quote:

psilocyboss said:
what happens if you forget to let it vent at the start? I thought i had it set to vent but I didn't and when i did, it only let air out for about 1.5mins. I did a 3 hour PC




You’ll be fine. Venting the IP is advised but is not quite as important as it is in the much larger canners. Those all have more air space to clear and hold at least 2 1/2 times more jars.

At the beginning (and for a couple of years) I did not vent my IP. Now, after following Stipe’s work on measuring sterilization, I do. But if I forgot I’d not lose any sleep over it. Especially with a 3 hour pressure cycle.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: DharmaForKarma]
    #28240762 - 03/22/23 04:04 AM (10 months, 2 days ago)

Venting at the beginning is very important.  Your PC timer should only start after at lease 10 min of venting.  I prefer 15-20 min.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: schpat]
    #28242778 - 03/23/23 10:29 AM (10 months, 20 hours ago)

Quote:

schpat said:
Venting at the beginning is very important.  Your PC timer should only start after at lease 10 min of venting.  I prefer 15-20 min.




Are you using a PC or an IP? With the IP venting is a good idea but not as important as with a PC.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: DharmaForKarma]
    #28242827 - 03/23/23 11:06 AM (10 months, 19 hours ago)

What seems to be the minimum water depth for a 3 hour pressure cook (plus 5 min vent)? How much does the water level seem to drop during a 3 hour cycle?

Trivet high? Deeper?

I'm doing a bunch of shallow, lightweight containers, and would rather not start out with them floating around if I can avoid it, but I also don't want to run dry before the time is up.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: eu-da-mon]
    #28242920 - 03/23/23 12:07 PM (10 months, 18 hours ago)

I've ran 3.5 cups of water for 3.5 hours with a 15 minute vent with no issues. Instruction manual says to not PC with less than 2 cups. I'd say 3 cups would be fine.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Way]
    #28242933 - 03/23/23 12:15 PM (10 months, 18 hours ago)

Instant pots are mostly sealed so you aren’t gonna lose much water compared to a traditional pc


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: MonoVision]
    #28243443 - 03/23/23 06:26 PM (10 months, 12 hours ago)

I go trivet high for 15 minute vent and 2:45 cook. There is plenty of water at the end. I’ve not measured though.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: DharmaForKarma]
    #28270856 - 04/10/23 08:52 AM (9 months, 13 days ago)

Question regarding when grain is dry enough after soaking.

Last night i did my first jar with WBS.  I soaked for 12 hours, drained, rinsed, etc.  In the strainer, there were no more drips.  I then put WBS into just a large tub i have nad put a fan on top to help air out.  I moved the WBS around with a spatula until i saw no more visible water at all on teh bottom of my container.  I grabbed afew handfuls and squeezed as hard as i could and no water drops anywhere.

Then, i put my lids on, wrapped top of lids with foil, put on instant pot for 3 hours on high.

Today i noticed water at the bottom of the jars.  Not a lot, but if you move the jar to an angle, you can see the water move.

Is this too wet or is having a minimal amount of water from the grain soaking ok at the bottom of the jar?  When i shake the jar stuff sticks to the side slightly also (i did not use any gypsum on the wbs)


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: kinadafz] * 1
    #28293555 - 04/24/23 06:47 PM (8 months, 30 days ago)

any tips for dehydrating with the instant pot?


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 1
    #28359112 - 06/14/23 12:11 AM (7 months, 11 days ago)

Glad I found this thread! Just purchased an instant pot, the pro model that we have here in Australia. 8L is big enough to fit 3-4 large mason jars and 6 half pint jars. It has a handy sterilise function for all my jars and tools, and is definitely an upgrade from my pot on the stove.

A little disappointed in the response of the "purists" when posting in the group about electric pressure cookers. Some of us just aren't ready for a 23q presto! With the amount of success people have with this method, I don't understand the hate electric cookers get. A 25-30 minute pressure cook gets the popcorn just right. I'm going to jar the it up and PC for 2 hours. Tomorrow I'm testing it out with a curry 🤤


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InvisibleFloret
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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: psilocyboss]
    #28408640 - 07/25/23 11:21 PM (6 months, 7 hours ago)

Quote:

psilocyboss said:
any tips for dehydrating with the instant pot?




Instant pots and air fryers is poor dehydrators. Consensus is to buy a dehydrator. Better than losing potency and risk mold due to poor drying.


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LAGM 2024


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: PudinLicous] * 2
    #28463174 - 09/09/23 09:31 AM (4 months, 16 days ago)

This thread deserves a bump. I found out that my Presto does not work on induction after moving from gas so I tried the instant pot. Friday I sterilized a pre-sealed small, 2 liter spawn bag of WBS in the IP for 3.5 hours, venting for 5 minutes. I will just leave it alone for a week or two and see what happens.


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If you have used a Miraculix Psilocybin QTest, could you please share your results?

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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Tiamo] * 1
    #28463973 - 09/10/23 03:47 AM (4 months, 16 days ago)

Standard instantpots will go to 15psi at the start, then back down. I have spoken before about repeatedly turning it off and on again, I have not tested it with a temperature probe but will but I think it does go through the 15psi start up cycle again.



There are sensors inside the IP on the bottom, they call them pressure sensors, I am not sure if they truly are but they can definitely be adjusted and the pressure/temp can be got higher. They are factory set and covered with white adhesive/silicone stuff, this can be scraped off and there are screws you can adjust. People get a C6, C6L or C6H error if they are faulty. Of course this is dangerous if you do not know what you are doing, but even if set to a much higher temp/pressure it is designed to just vent if it gets too high.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: blackout]
    #28464409 - 09/10/23 01:56 PM (4 months, 15 days ago)

Mine is 12.5 psi, but I never saw an issue just increasing the time by half an hour or an hour. Or maybe just 50 minutes doin agar


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Eminence]
    #28464416 - 09/10/23 02:03 PM (4 months, 15 days ago)

I have a knockoff(Farberware) that says it runs at 15PSI for the rice/risotto setting only. It's only a 14-18 minute cycle though, so that would be a pain.

I just stick with the 2hr cycle.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Tiamo]
    #28469515 - 09/15/23 05:19 AM (4 months, 11 days ago)

Quote:

Tiamo said:
This thread deserves a bump. I found out that my Presto does not work on induction after moving from gas so I tried the instant pot. Friday I sterilized a pre-sealed small, 2 liter spawn bag of WBS in the IP for 3.5 hours, venting for 5 minutes. I will just leave it alone for a week or two and see what happens.




A week later the bag looks good. I will knock it up with some LC today. (Edit: Knocked it up with 2 mL PE LC)

I will mention that the grains were a bit on the wet side and some were burst. I followed the prep from this thread, but I did rinse and remove floaters before. I also may not have let them steam long enough.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


Edited by Tiamo (09/15/23 08:34 AM)


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Tiamo]
    #28477395 - 09/21/23 07:17 AM (4 months, 4 days ago)

The bag never took off. I see a tiny bit of white, but also a slimy bacterial layer. I suspect the sterilization was not enough.

The LC on two other larger bags of rye sterilized in a Presto is doing much better.

Going to try jars in the IP in stead.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Tiamo]
    #28477521 - 09/21/23 10:19 AM (4 months, 4 days ago)

Tiamo, I use an IP for quart jars exclusively and have never had contamination related to sterilization.  My IP holds 4 quart jars and 2:45 has been enough time for WBS and oats, 2:30 for millet. I’m at around 5,500 feet in elevation which adds a bit more time.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: DharmaForKarma]
    #28477652 - 09/21/23 01:15 PM (4 months, 4 days ago)

Quote:

DharmaForKarma said:
I’m at around 5,500 feet in elevation which adds a bit more time.




That and the lower pressure of an IP. I run mine for 4 hours for oats.  I haven't wanted to "see what I can get away with." 

With elevation we're getting less than 10 PSI rather than 15.  That's why I double the (longest) time used with a 15 PSI PC.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: DharmaForKarma]
    #28477718 - 09/21/23 02:18 PM (4 months, 4 days ago)

Quote:

DharmaForKarma said:
Tiamo, I use an IP for quart jars exclusively and have never had contamination related to sterilization.  My IP holds 4 quart jars and 2:45 has been enough time for WBS and oats, 2:30 for millet. I’m at around 5,500 feet in elevation which adds a bit more time.




I don't doubt it. I just wanted to do an experiment and share results for others. :smile:

I am below sea level.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Kinoko314]
    #28477726 - 09/21/23 02:29 PM (4 months, 4 days ago)

Quote:

Kinoko314 said:
Quote:

DharmaForKarma said:
I’m at around 5,500 feet in elevation which adds a bit more time.




That and the lower pressure of an IP. I run mine for 4 hours for oats.  I haven't wanted to "see what I can get away with." 

With elevation we're getting less than 10 PSI rather than 15.  That's why I double the (longest) time used with a 15 PSI PC.




Before I started I did a bunch of experiments including weighing sterilized oats for water content and putting autoclave tape inside of jars to confirm adequate temperature. (Stipe has since confirmed that IPs do sterilize jars with his more rigorous testing equipment and methodology.) Anything over 90 minutes and the temp hits 250° F but the oats didn’t look right until 2:30. Another fifteen minutes and they had the hydration I was looking for. At 3 hours they are beginning to burst.

Elevation does affect timing, for IPs and PCs alike, due to pressure reduction. 15 psi is not a magic number for sterilization though it’s a nice number to work with. It all comes down to transference of energy via time under temperature. I want to run jars as long as it takes, no less and no more.


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Re: Pudin's Instant Pot Tek - pasteruization, sterilization, grain prep, and beyond [Re: Tiamo]
    #28477732 - 09/21/23 02:37 PM (4 months, 4 days ago)

Quote:

Tiamo said:

I am below sea level.




Where I am water boils at 94°C. Messes with cooking and baking, too.


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