AZ Starman2112 said:

I would really think you peeps have some sort of a degree in science. Y’all are like wizards.

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Well, your sperm is a mono, so is your wife's egg, they fuse and become a dikaryon, your child is the fruit

Or that is what I thought, and is what I'm trying to find out, but both Nichrome and tree frog are keeping me waiting

Kloakk said:

AZ Starman2112 said:

I would really think you peeps have some sort of a degree in science. Y’all are like wizards.

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Well, your sperm is a mono, so is your wife's egg, they fuse and become a dikaryon, your child is the fruit

Or that is what I thought, and is what I'm trying to find out, but both Nichrome and tree frog are keeping me waiting

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tree frog said:

@kloakk two monos clamping is a dikaryon.  And they may be vegetatively compatible but not good mating partners.  In which case they might find a better nuclei to clamp with later in the lifecycle. 

They're also amorous prior to making mushrooms, which is the parasexual cycle I mentioned above.  Because two monos might not be good mating partners, it's generally easier to clone a good fruiter from a plate that has had more opportunities to find a mate, than to run out a bunch of mono/mono dikaryons.

To isolate a single strain, take small transfers from leading organized growth.  Larger wedges and disorganized growth are more likely to have multiple strains.  Using grab and drags or other germination techniques makes this easier to do with minimal transfers.  And can even be used to pull monos (which really isn't hard, but a microscope helps to confirm).

Variety is the preferred name over strain for a large set of DNA like Penis Envy or B+.  Strains are active dikaryons on a plate, in a jar, etc.

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Archespore said:

Nichrome said:

Isolating dikaryons is not hard or equipment intensive.

Personally I find diverse, cooperative colonies to be more productive and posses more longevity than isolated dikaryons. Diverse colonies have diverse metabolism that is shared collectively.

Select for areas without an internal power struggle. Identify good cooperation, treat it well, and run with it.

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You sound like a mushroom whisperer ;_;

How? How do?

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Kloakk said:

tree frog said:

@kloakk two monos clamping is a dikaryon.  And they may be vegetatively compatible but not good mating partners.  In which case they might find a better nuclei to clamp with later in the lifecycle. 

They're also amorous prior to making mushrooms, which is the parasexual cycle I mentioned above.  Because two monos might not be good mating partners, it's generally easier to clone a good fruiter from a plate that has had more opportunities to find a mate, than to run out a bunch of mono/mono dikaryons.

To isolate a single strain, take small transfers from leading organized growth.  Larger wedges and disorganized growth are more likely to have multiple strains.  Using grab and drags or other germination techniques makes this easier to do with minimal transfers.  And can even be used to pull monos (which really isn't hard, but a microscope helps to confirm).

Variety is the preferred name over strain for a large set of DNA like Penis Envy or B+.  Strains are active dikaryons on a plate, in a jar, etc.

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I'm mostly talking about semantics here, like the definition of a word has to mean the same for everyone if we are to understand each other. So 2 monos fusing makes a dykarion, so if 3 or more monos fuse it should have a different name or everything just becomes muddy.

Like I said, I don't remember where I read it, it was on the org. Point being that a strain is 2 monos fusing making a dikaryon and that dikaryon is a strain. I have no clue of this is true or not, but naming different things the same sounds confusing and is why I asked.

And when you say find a better nucleic to clamp with, I am talking about putting 2 spores to a plate, they fuse and become dikaryon. Would there be any other nucleic to clamp with on that plate?

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Dj_karma_magnet said:

Thank you, I’m a little confused, should I toss the part that is inconsistent and transfer the part that is consistent? Thanks


Also, is anyone here incubating their plates or do you just let them sit at room temp? Any cheap devices to incubate ?

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Kloakk said:

I'm mostly talking about semantics here, like the definition of a word has to mean the same for everyone if we are to understand each other. So 2 monos fusing makes a dykarion, so if 3 or more monos fuse it should have a different name or everything just becomes muddy.

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Dikaryon means two separate nuclei in a single cell.  Monokaryon means one nuclei.

Fusion doesn't typically take place until spores are made.  And during vegetarian vegetation, a dikaryon may meet a mono or another dikaryon that is more compatible for mating.  But the result of the cross is still a two nuclei dikaryon.

The process by which this happens I don't understand well.

But it's here if you want to dig into it.

https://davidmoore.org.uk/21st_century_guidebook_to_fungi_platinum/Ch07_05.htm

Anyway, I would call these green points dikaryons.  Even the fourth one that emmerged late from two others.  And I mostly use isolate as a verb.  I.e. transferring small wedges from one of these strains is isolating them from the others.



If I took this blue circle of three strains to a jar, I would call it a multi-strain colony.

tree frog said:

Dikaryon means two separate nuclei in a single cell.  Monokaryon means one nuclei.

Fusion doesn't typically take place until spores are made.  And during vegetarian vegetation, a dikaryon may meet a mono or another dikaryon that is more compatible for mating.  But the result of the cross is still a two nuclei dikaryon.

The process by which this happens I don't understand well.

But it's here if you want to dig into it.

https://davidmoore.org.uk/21st_century_guidebook_to_fungi_platinum/Ch07_05.htm

Anyway, I would call these green points dikaryons.  Even the fourth one that emmerged late from two others.  And I mostly use isolate as a verb.  I.e. transferring small wedges from one of these strains is isolating them from the others.



If I took this blue circle of three strains to a jar, I would call it a multi-strain colony.

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I think I understand So at the plasmogamy stage the cells will fuse, but the nuclei won't, bringing two haploid nuclei close together in the same cell, using a clamp right?

And fusion of the nuclei happens in the gills before the spores are dropped, right?

So then mono + mono = dikaryon if clamps are formed. Dikaryon is what I assumed was a strain. When one or more monos or dikaryons mate with that dikaryon it is a multi strain colony.

Isolating is one thing(redusing the number of dikaryon strains from a multi-strain colony), but an isolate(one single dikaryon) I imagine is harder to do with just our eyes and a scalpel or?

Sorry for all the questions, but I would like to understand this, I will take a look at the link as well, thanks tree

Kloakk said:

I think I understand So at the plasmogamy stage the cells will fuse, but the nuclei won't, bringing two haploid nuclei close together in the same cell, using a clamp right?

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Quote:

And fusion of the nuclei happens in the gills before the spores are dropped, right?

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Quote:

When one or more monos or dikaryons mate with that dikaryon it is a multi strain colony.

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Quote:

Isolating is one thing(redusing the number of dikaryon strains from a multi-strain colony), but an isolate(one single dikaryon) I imagine is harder to do with just our eyes and a scalpel or?

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Quote:

Sorry for all the questions, but I would like to understand this, I will take a look at the link as well, thanks tree

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Tropical said:

Would you bother with a plate like that with a myc that s not very dense?

If yes why?
Which sectors would you take?

Take care everyone!

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