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Re: AGAR ENVY! (Anything and All things agar!) [Re: fungitobewith]
    #29178815 -

Nichrome said:
Flying solo back there.





fungitobewith said:
Knee deep


Holy shit, that's what's up.. This is the true envy..

Tax:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk] * 1
    #29178944 -

Nichrome said:
Fun fact: tartaric acid prevents the agarose in agar from forming a polymer matrix.


Kloakk said:
Nichrome said:
Flying solo back there.





fungitobewith said:
Knee deep


Holy shit, that's what's up.. This is the true envy..

Tax:



That is only one pile, I have another pile in the other paddock. I need to make the largest outdoor bed ever! :yields:


--------------------

Check out my My Trade List  :opensign:

Check out my wanted Strain List  :opensign:



Edited by fungitobewith (04/04/25 08:06 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fungitobewith]
    #29178951 -

fungitobewith said:
That is only one pile, I have another pile in the other paddock. I need to make the largest outdoor bed ever! :yields:


Man, I feel that, I wish I could do that, but I'm in a place with -20 Celsius 4 months of the year. So no can do. So when you do a big bed, do one for me.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: unstuckPilgrim]
    #29179083 -

unstuckPilgrim said:
Thanks!  I'm sure I'll be back to ask for more input on the next round.



hey dont apologize for asking questions friendo its what we are here for. 

youre maybe two transfers away from a clean culture and youre on the right track  and asking the right questions. keep asking.  stay curious

thanks for being with us. we appreciate you.  z


--------------------
Need a lift?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk] * 3
    #29179122 -

Bookmarked as: fundamental discussions on mushroom cultivation, part IV-XX: community and personal hygiene. Archive material.


--------------------
Species grown 2025-26
https://www.shroomery.org/forums/showflat.php/Number/29367720#29367720

Collection of trip reports
https://www.shroomery.org/forums/showflat.php/Number/29242884#29242884

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fungitobewith]
    #29179210 -

fungitobewith said:





That's some mycoporn friend. Straight up mycoporn...



--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DV8DUG] * 2
    #29179300 -

DV8DUG said:
fungitobewith said:





That's some mycoporn friend. Straight up mycoporn...




:flexin:


--------------------

Check out my My Trade List  :opensign:

Check out my wanted Strain List  :opensign:



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Re: AGAR ENVY! (Anything and All things agar!) [Re: fungitobewith] * 4
    #29179341 -

fungitobewith said:
Knee deep


The lawnvac is great for shit shredding


Edited by LewDoja (04/05/25 10:31 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LewDoja] * 4
    #29179373 -

Should have taken pics but last week I used a weed whacker inside a metal trashcan.

Chopped that shit up good 👍


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #29179539 -

Noob agar journey continues. I made some no pours with glass dishes. I Think I'm going to move on to oven tek...

Things vere quite moist :rolleyes:. Miracle if my cloning is succesful.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MightBeARealPerson]
    #29179553 -

Learning lesson & noob mistake!!!!

I poured 52 new black & blue plates yesterday. I learned not to put 500ml of liquid agar in your 500ml media bottles and then put them in the PC. Boy did I have a big mess! I probably could have poured well over 60 plates, maybe 70. My media bottles all boiled over inside creating a real mess and a real PITA to clean up. I’m thinking I should stay in the 350-400ml range next time. First time I did around 350ml per bottle and had no issues. Figured I’d do a double batch of 2000ml because I have 4 bottles. That no worky! On a positive note, the plates came out great though!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112]
    #29179562 -

Yeah, 400 ml per 500 ml bottle is the trick. You may be able to get away with a little more, but at least for me, 400 ml is enough for 20 of the 90 mm plates or 40 of the 60 mm plates, so it's actually a pretty convenient amount both for calculations (multiple of 100) and for pouring (multiple of typical petri dish sleeve size or the number of autoclavable ones that fit in a jar).

I'm assuming your 52 plates were from two bottles, not all four - otherwise those must have been really deep pours!


--------------------
LAGM 2026 grow log | My library/trade list

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MightBeARealPerson]
    #29179571 -

MightBeARealPerson said:
Noob agar journey continues. I made some no pours with glass dishes. I Think I'm going to move on to oven tek...

Things vere quite moist :rolleyes:. Miracle if my cloning is succesful.


Never move on to oven tek.  Only move on from it if unfortunate enough to not avoid altogether.


--------------------
The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Phaethon]
    #29179576 -

Phaethon said:
Yeah, 400 ml per 500 ml bottle is the trick. You may be able to get away with a little more, but at least for me, 400 ml is enough for 20 of the 90 mm plates or 40 of the 60 mm plates, so it's actually a pretty convenient amount both for calculations (multiple of 100) and for pouring (multiple of typical petri dish sleeve size or the number of autoclavable ones that fit in a jar).

I'm assuming your 52 plates were from two bottles, not all four - otherwise those must have been really deep pours!


400ml sounds like a better bet! They were 90mm plates, and yes all four. I pour about half the depth of the plate, which is probably too much. This is only my second time pouring plates. Next time I'll pour about 1/3 the height, or should I just cover the bottom with a thin layer? which I guess would be about 1/4 the height.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nocap]
    #29179602 -

nocap said:
MightBeARealPerson said:
Noob agar journey continues. I made some no pours with glass dishes. I Think I'm going to move on to oven tek...

Things vere quite moist :rolleyes:. Miracle if my cloning is succesful.


Never move on to oven tek.  Only move on from it if unfortunate enough to not avoid altogether.


What is wrong with oven tek? And then what is the best way to use glass dishes? I also have single use petris but I don't like the amount of trash.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MightBeARealPerson]
    #29179621 -

MightBeARealPerson said:
nocap said:
MightBeARealPerson said:
Noob agar journey continues. I made some no pours with glass dishes. I Think I'm going to move on to oven tek...

Things vere quite moist :rolleyes:. Miracle if my cloning is succesful.


Never move on to oven tek.  Only move on from it if unfortunate enough to not avoid altogether.


What is wrong with oven tek? And then what is the best way to use glass dishes? I also have single use petris but I don't like the amount of trash.


There may be some confusion by your use of “oven tek.” That usually means working in front of a warm oven instead of in an SAB or flow hood. A very unsatisfactory method of sterile work. I’m hoping you are describing something else when you say oven tek. Perhaps dry heat sterilizing your glass plates in the oven to pour agar into later?


--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: RoscoeReturns] * 3
    #29179633 -

RoscoeReturns said:
MightBeARealPerson said:
nocap said:
MightBeARealPerson said:
Noob agar journey continues. I made some no pours with glass dishes. I Think I'm going to move on to oven tek...

Things vere quite moist :rolleyes:. Miracle if my cloning is succesful.


Never move on to oven tek.  Only move on from it if unfortunate enough to not avoid altogether.


What is wrong with oven tek? And then what is the best way to use glass dishes? I also have single use petris but I don't like the amount of trash.


There may be some confusion by your use of “oven tek.” That usually means working in front of a warm oven instead of in an SAB or flow hood. A very unsatisfactory method of sterile work. I’m hoping you are describing something else when you say oven tek. Perhaps dry heat sterilizing your glass plates in the oven to pour agar into later?


Yes indeed I meant dry heat sterilizing. I encountered unusual influx of alcohol in my body and got confused. I drink so rarely that even a beer or two is quite disorienting.

Anyways. I'm using a SAB and last single use petris I poured turned out to be fine, so I might try pouring with glass ones also. Still quite clumsy at everything agar but slowly getting better.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: RoscoeReturns] * 2
    #29179635 -

false alarm


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112] * 2
    #29179642 -

AZ Starman2112 said:
Phaethon said:
Yeah, 400 ml per 500 ml bottle is the trick. You may be able to get away with a little more, but at least for me, 400 ml is enough for 20 of the 90 mm plates or 40 of the 60 mm plates, so it's actually a pretty convenient amount both for calculations (multiple of 100) and for pouring (multiple of typical petri dish sleeve size or the number of autoclavable ones that fit in a jar).

I'm assuming your 52 plates were from two bottles, not all four - otherwise those must have been really deep pours!


400ml sounds like a better bet! They were 90mm plates, and yes all four. I pour about half the depth of the plate, which is probably too much. This is only my second time pouring plates. Next time I'll pour about 1/3 the height, or should I just cover the bottom with a thin layer? which I guess would be about 1/4 the height.


1/3 to 1/2 I think is about what I do. The media pretty much comes up to the level of the lid rim when it's on...which is no help to you while pouring :lol: but a good reference to check after a pour.

There's nothing wrong with thicker agar, and all the ingredients are cheap. Figure out what works best for you and don't sweat it. :smile:


--------------------
LAGM 2026 grow log | My library/trade list

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LewDoja]
    #29179715 -

LewDoja said:
fungitobewith said:
Knee deep


The lawnvac is great for shit shredding




The goats and the horse, for some reason like to play king of the hill on the pile, its flat as a pancake. :baseballfuck: now I have to put it in a pile again :laugh2: But its nice and broke up :shrug: The lawnvac is a great idea...


--------------------

Check out my My Trade List  :opensign:

Check out my wanted Strain List  :opensign:



Edited by fungitobewith (04/05/25 06:41 PM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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