AGAR ENVY! (Anything and All things agar!) - Mushroom Cultivation - Shroomery Message Board

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Kloakk
Strange

Registered: 10/21/24
Posts: 5,888
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Last seen: 33 minutes, 12 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: fungitobewith]
#29178815 - 04/04/25 05:31 PM (1 year, 5 months ago)

Nichrome said:

Flying solo back there.

fungitobewith said:

Knee deep

Holy shit, that's what's up.. This is the true envy..

Tax:

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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett

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fungitobewith
Fungi To Be With

Registered: 07/15/07
Posts: 979
Loc: Rolling Stone, PA/Some Place C...

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Kloakk]

1
#29178944 - 04/04/25 08:03 PM (1 year, 5 months ago)

Nichrome said:

Fun fact: tartaric acid prevents the agarose in agar from forming a polymer matrix.

Kloakk said:

Nichrome said:

Flying solo back there.

fungitobewith said:

Knee deep

Holy shit, that's what's up.. This is the true envy..

Tax:

That is only one pile, I have another pile in the other paddock. I need to make the largest outdoor bed ever! :yields:

:yields:

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Edited by fungitobewith (04/04/25 08:06 PM)

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Kloakk
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: fungitobewith]
#29178951 - 04/04/25 08:15 PM (1 year, 5 months ago)

fungitobewith said:

That is only one pile, I have another pile in the other paddock. I need to make the largest outdoor bed ever!

Man, I feel that, I wish I could do that, but I'm in a place with -20 Celsius 4 months of the year. So no can do. So when you do a big bed, do one for me.

--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett

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BeefExtreme
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: unstuckPilgrim]
#29179083 - 04/04/25 11:52 PM (1 year, 5 months ago)

unstuckPilgrim said:

Thanks! I'm sure I'll be back to ask for more input on the next round.

hey dont apologize for asking questions friendo its what we are here for.

youre maybe two transfers away from a clean culture and youre on the right track and asking the right questions. keep asking. stay curious

thanks for being with us. we appreciate you. z

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Churpee Choozle
Grossly contamed

Registered: 11/11/24
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Kloakk]

3
#29179122 - 04/05/25 02:57 AM (1 year, 5 months ago)

Bookmarked as: fundamental discussions on mushroom cultivation, part IV-XX: community and personal hygiene. Archive material.

--------------------
Species grown 2025-26
https://www.shroomery.org/forums/showflat.php/Number/29367720#29367720

Collection of trip reports
https://www.shroomery.org/forums/showflat.php/Number/29242884#29242884

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DV8DUG
Hyphal Exhibitionist

Registered: 12/27/23
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: fungitobewith]
#29179210 - 04/05/25 06:54 AM (1 year, 5 months ago)

fungitobewith said:

That's some mycoporn friend. Straight up mycoporn...

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Agar Plates and Removing Hidden Contaminants - A Visual NotebookRecognizing Contamination in Grain Jars - A Visual NotebookContaminated Condiment Cup Cleanup v1.0

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fungitobewith
Fungi To Be With

Registered: 07/15/07
Posts: 979
Loc: Rolling Stone, PA/Some Place C...

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DV8DUG]

2
#29179300 - 04/05/25 09:43 AM (1 year, 5 months ago)

DV8DUG said:

fungitobewith said:

That's some mycoporn friend. Straight up mycoporn...

:flexin:

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LewDoja
mad $cientist, ganjacologist

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: fungitobewith]

4
#29179341 - 04/05/25 10:30 AM (1 year, 5 months ago)

fungitobewith said:

Knee deep

The lawnvac is great for shit shredding

Edited by LewDoja (04/05/25 10:31 AM)

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mushboy
modboy

Registered: 04/24/05
Posts: 40,543
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: LewDoja]

4
#29179373 - 04/05/25 10:56 AM (1 year, 5 months ago)

Should have taken pics but last week I used a weed whacker inside a metal trashcan.

Chopped that shit up good 👍

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MightBeARealPerson
Old toe dipper ready to dive

Registered: 05/18/23
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#29179539 - 04/05/25 02:28 PM (1 year, 5 months ago)

Noob agar journey continues. I made some no pours with glass dishes. I Think I'm going to move on to oven tek...

Things vere quite moist :rolleyes:

:rolleyes:

. Miracle if my cloning is succesful.

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AZ Starman2112
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: MightBeARealPerson]
#29179553 - 04/05/25 02:48 PM (1 year, 5 months ago)

Learning lesson & noob mistake!!!!

I poured 52 new black & blue plates yesterday. I learned not to put 500ml of liquid agar in your 500ml media bottles and then put them in the PC. Boy did I have a big mess! I probably could have poured well over 60 plates, maybe 70. My media bottles all boiled over inside creating a real mess and a real PITA to clean up. I’m thinking I should stay in the 350-400ml range next time. First time I did around 350ml per bottle and had no issues. Figured I’d do a double batch of 2000ml because I have 4 bottles. That no worky! On a positive note, the plates came out great though!

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Phaethon
Hobbyist

Registered: 07/04/22
Posts: 1,268
Loc: Colorado

Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]
#29179562 - 04/05/25 03:03 PM (1 year, 5 months ago)

Yeah, 400 ml per 500 ml bottle is the trick. You may be able to get away with a little more, but at least for me, 400 ml is enough for 20 of the 90 mm plates or 40 of the 60 mm plates, so it's actually a pretty convenient amount both for calculations (multiple of 100) and for pouring (multiple of typical petri dish sleeve size or the number of autoclavable ones that fit in a jar).

I'm assuming your 52 plates were from two bottles, not all four - otherwise those must have been really deep pours!

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nocap
for real

Registered: 09/29/24
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: MightBeARealPerson]
#29179571 - 04/05/25 03:16 PM (1 year, 5 months ago)

MightBeARealPerson said:

Noob agar journey continues. I made some no pours with glass dishes. I Think I'm going to move on to oven tek...

Things vere quite moist . Miracle if my cloning is succesful.

Never move on to oven tek. Only move on from it if unfortunate enough to not avoid altogether.

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AZ Starman2112
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Phaethon]
#29179576 - 04/05/25 03:35 PM (1 year, 5 months ago)

Phaethon said:

Yeah, 400 ml per 500 ml bottle is the trick. You may be able to get away with a little more, but at least for me, 400 ml is enough for 20 of the 90 mm plates or 40 of the 60 mm plates, so it's actually a pretty convenient amount both for calculations (multiple of 100) and for pouring (multiple of typical petri dish sleeve size or the number of autoclavable ones that fit in a jar).

I'm assuming your 52 plates were from two bottles, not all four - otherwise those must have been really deep pours!

400ml sounds like a better bet! They were 90mm plates, and yes all four. I pour about half the depth of the plate, which is probably too much. This is only my second time pouring plates. Next time I'll pour about 1/3 the height, or should I just cover the bottom with a thin layer? which I guess would be about 1/4 the height.

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MightBeARealPerson
Old toe dipper ready to dive

Registered: 05/18/23
Posts: 22
Loc: Scandinavia
Last seen: 10 days, 18 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: nocap]
#29179602 - 04/05/25 04:21 PM (1 year, 5 months ago)

nocap said:

MightBeARealPerson said:

Noob agar journey continues. I made some no pours with glass dishes. I Think I'm going to move on to oven tek...

Things vere quite moist . Miracle if my cloning is succesful.

Never move on to oven tek. Only move on from it if unfortunate enough to not avoid altogether.

What is wrong with oven tek? And then what is the best way to use glass dishes? I also have single use petris but I don't like the amount of trash.

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RoscoeReturns
We’ve outstayed our welcome

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: MightBeARealPerson]
#29179621 - 04/05/25 04:42 PM (1 year, 5 months ago)

MightBeARealPerson said:

nocap said:

MightBeARealPerson said:

Noob agar journey continues. I made some no pours with glass dishes. I Think I'm going to move on to oven tek...

Things vere quite moist . Miracle if my cloning is succesful.

Never move on to oven tek. Only move on from it if unfortunate enough to not avoid altogether.

What is wrong with oven tek? And then what is the best way to use glass dishes? I also have single use petris but I don't like the amount of trash.

There may be some confusion by your use of “oven tek.” That usually means working in front of a warm oven instead of in an SAB or flow hood. A very unsatisfactory method of sterile work. I’m hoping you are describing something else when you say oven tek. Perhaps dry heat sterilizing your glass plates in the oven to pour agar into later?

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MightBeARealPerson
Old toe dipper ready to dive

Registered: 05/18/23
Posts: 22
Loc: Scandinavia
Last seen: 10 days, 18 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: RoscoeReturns]

3
#29179633 - 04/05/25 04:53 PM (1 year, 5 months ago)

RoscoeReturns said:

MightBeARealPerson said:

nocap said:

MightBeARealPerson said:

Noob agar journey continues. I made some no pours with glass dishes. I Think I'm going to move on to oven tek...

Things vere quite moist . Miracle if my cloning is succesful.

Never move on to oven tek. Only move on from it if unfortunate enough to not avoid altogether.

What is wrong with oven tek? And then what is the best way to use glass dishes? I also have single use petris but I don't like the amount of trash.

There may be some confusion by your use of “oven tek.” That usually means working in front of a warm oven instead of in an SAB or flow hood. A very unsatisfactory method of sterile work. I’m hoping you are describing something else when you say oven tek. Perhaps dry heat sterilizing your glass plates in the oven to pour agar into later?

Yes indeed I meant dry heat sterilizing. I encountered unusual influx of alcohol in my body and got confused. I drink so rarely that even a beer or two is quite disorienting.

Anyways. I'm using a SAB and last single use petris I poured turned out to be fine, so I might try pouring with glass ones also. Still quite clumsy at everything agar but slowly getting better.

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catnip40
Puppet∴Master

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: RoscoeReturns]

2
#29179635 - 04/05/25 04:54 PM (1 year, 5 months ago)

false alarm

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Phaethon
Hobbyist

Registered: 07/04/22
Posts: 1,268
Loc: Colorado

Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]

2
#29179642 - 04/05/25 04:56 PM (1 year, 5 months ago)

AZ Starman2112 said:

Phaethon said:

Yeah, 400 ml per 500 ml bottle is the trick. You may be able to get away with a little more, but at least for me, 400 ml is enough for 20 of the 90 mm plates or 40 of the 60 mm plates, so it's actually a pretty convenient amount both for calculations (multiple of 100) and for pouring (multiple of typical petri dish sleeve size or the number of autoclavable ones that fit in a jar).

I'm assuming your 52 plates were from two bottles, not all four - otherwise those must have been really deep pours!

400ml sounds like a better bet! They were 90mm plates, and yes all four. I pour about half the depth of the plate, which is probably too much. This is only my second time pouring plates. Next time I'll pour about 1/3 the height, or should I just cover the bottom with a thin layer? which I guess would be about 1/4 the height.

1/3 to 1/2 I think is about what I do. The media pretty much comes up to the level of the lid rim when it's on...which is no help to you while pouring :lol:

:lol:

but a good reference to check after a pour.

There's nothing wrong with thicker agar, and all the ingredients are cheap. Figure out what works best for you and don't sweat it. :smile:

:smile:

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fungitobewith
Fungi To Be With

Registered: 07/15/07
Posts: 979
Loc: Rolling Stone, PA/Some Place C...

Re: AGAR ENVY! (Anything and All things agar!)

[Re: LewDoja]
#29179715 - 04/05/25 06:37 PM (1 year, 5 months ago)

LewDoja said:

fungitobewith said:

Knee deep

The lawnvac is great for shit shredding

The goats and the horse, for some reason like to play king of the hill on the pile, its flat as a pancake. :baseballfuck:

:baseballfuck:

now I have to put it in a pile again :laugh2:

:laugh2:

But its nice and broke up :shrug:

:shrug:

The lawnvac is a great idea...

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Edited by fungitobewith (04/05/25 06:41 PM)

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