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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhenoDreamers] * 4
    #29109398 -

nasopharyngeal swabs work well. I just tried them on these tamps. Picked up and deposited spores very efficiently. I do wish they weren't plastic/nylon, I prefer degradable materials.





--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme] * 2
    #29109402 -

BeefSupremeJr said:
deng fahts.  this new yeti is a ropey sombish






--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PuffM4gicDr4gon] * 1
    #29109447 -

I went a little overboard on germ plates. I wasn't expecting them all to take off but here we are. This agar thing is pretty fun even though it has exposed how incredibly impatient I am. Any suggestions on which plates I should focus on for transfers?


Edited by bchino (01/31/25 07:07 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Scemilope] * 4
    #29109457 -

I'm ashamed of my environmental impact :frown:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bchino] * 2
    #29109462 -

bchino said:
I went a little overboard on germ plates. I wasn't expecting them all to take off but here we are. This agar this is pretty fun even though its exposed how incredibly impatient I am. Any suggestions on which plates I should focus on for transfers?





They all look great. Pick areas of nice clean uniform growth and the spots with the most dense rhizomorphs

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhenoDreamers] * 2
    #29109566 -

SupaThaRipper said:
I'm ashamed of my environmental impact :frown:


A symptom of functioning morals :lol:

It’s what made me switch to reusable plates finally


--------------------
𝕴𝖋 𝕴 π–π–†π–›π–Š π–˜π–Šπ–Šπ–“ π–‹π–šπ–—π–™π–π–Šπ–— π–Žπ–™ π–Žπ–˜ π–‡π–ž π–˜π–™π–†π–“π–‰π–Žπ–“π–Œ π–šπ–•π–”π–“ π–™π–π–Š π–˜π–π–”π–šπ–‘π–‰π–Šπ–—π–˜ 𝖔𝖋 π•²π–Žπ–†π–“π–™π–˜

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri]
    #29109887 -

I kind of think this looks neat and uniform, but should I be worried about the different growth between around 4 - 8?



edit. Too me it looks fine, just different. And I still haven’t tried figuring out how can I get rid of condensation. It’s not so much after pour. I’m just guessing the temp fluctuates quite a bit because of the central heating here.

edit edit. Just noticed that I’ve also somehow poured the plate a bit thinner there, maybe that’s all it is.

Edited by eilauver (02/01/25 06:11 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: eilauver]
    #29109997 -

Looking to start with agar. Well, in the mushroom context - I'm a lab technician and I've worked in a couple of microbiology labs during my career, and I've worked with petri dishes and agar etc, so I'm not a complete noob :laugh:

I had a spore syringe from a reputable seller/company (fwiw, they're based in a country where shrooms are legal to cultivate and possess for personal use, and they sell spores and mycelium for a lot of different edible mushroom species), and I used what was left of the spores (after PF tek cakes) to try if a liquid culture would work. Turns out it did, it's several weeks old now and I've kept it stored in the cold.

The PFtek cakes failed, but I still have that LC. FIgured I might as well try that on some agar next, before trying to get another print or syringe.


--------------------
You are a prisoner of the road you follow
Only the untrodden snowdrift is free
- A. Hellaakoski

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Sarnath]
    #29110008 -

Just fucking do it!
(Re: join a cult with me)
- Adam Sandler


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #29110013 -

LadysKnight said:
Just fucking do it!
(Re: join a cult with me)
- Adam Sandler


I'm in a lot of cults. Highlander, Gov't Mule, Dudeism... :laugh:


--------------------
You are a prisoner of the road you follow
Only the untrodden snowdrift is free
- A. Hellaakoski

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Sarnath]
    #29110043 -

Sarnath said:
LadysKnight said:
Just fucking do it!
(Re: join a cult with me)
- Adam Sandler


I'm in a lot of cults. Highlander, Gov't Mule, Dudeism... :laugh:


I understand that GMule is a band, but tell me about the others.



--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #29110234 -

Another question for you guys. Attempting a clone here, second transfer. Growth is all over the place. Where would you take transfers?



First instinct would be the rhizo growth on top of the left one, right? But is it too ropey?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: eilauver] * 1
    #29110236 -

What does the original plate look like?


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #29110261 -



Hey for this India woodlover where exactly is best place to catch that rhizomorfic growth 7 to 9 oclock??

Like middle of growth towards edge of plate?
Or at ends with the rhizo tips?

Or both?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #29110280 -

Smellyhobbit said:
What does the original plate look like?


Unfortunately I've already thrown out the plate with the actual stipe biopsy. I still have the previous transfer, but this has grown to quite a mess by now.



I guess maybe I was a bit too eager with the amount of material I scraped from the stipe. I think I even took tree samples to the T0.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: eilauver] * 1
    #29110296 -

eilauver said:
Smellyhobbit said:
What does the original plate look like?


Unfortunately I've already thrown out the plate with the actual stipe biopsy. I still have the previous transfer, but this has grown to quite a mess by now.



I guess maybe I was a bit too eager with the amount of material I scraped from the stipe. I think I even took tree samples to the T0.


My advice is to not toss the actual clone plate. Tissue plates are usually 1) totally clean if you did them right 2) what you’re after genetically, so there’s no need to transfer and 3) most likely in the cabinet to produce plate pins.

Stipe tissue is sterile. I’ve never had a clone contam unless I did something super wrong.

Keep them bad boys. Transfer when you want to expand.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #29110299 -

Doh.. Makes perfect sense to be honest. I kind of presumed that, I guess I was just a bit worried that I'm messing something up. Also it was one finicky hollow stemmed pf-cake's shroom. More of a practice one than anything of real value, to be honest.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: eilauver] * 1
    #29110303 -

Well the good news is there’s not really a wrong answer with your plate as is. Transfer with your heart and let em rip


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #29110305 -

So what do you think is going on here? These are F1, T1 swabs from a giant mutant from my mutant strain The Thing, which was a mutated clone of my Husky, which is a standard cube.







This was the original mutant, and what plates looked like.





And those tubs usually came out a bit like this



These T1 plates, despite being from a spore swab, are creating mutant looking pins. Finally I have some of this on grains, so I should find out the outcome within a few weeks.

I was honestly expecting a revert back to a more standard cube after going back to spores, but perhaps this F1 is going to be a reliable "strain". Are there cases of mutant strains yielding reliable mutant offspring via spores? I know enigma doesn't spore at all.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Huskies] * 5
    #29110677 -

Testing out if doing a cabin sequestering on scalloped cultures fixes the problem.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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