ReverendMyc said:

I ran the full gauntlet of recovery efforts on the ones I had trouble with. I believe I tried hydrating for 24 & 48 hours and for 1 week without success. But they were mailed to me during very high temperatures so that may be the culprit. After those failed, I just moved on instead of testing the limits. That is why I want to give these ones that I know haven't been exposed to high temps another shot.

I have tested agar baggies at local high temperatures and even fresh hydrated myc died in 75% of the samples tested 110f(44c) afternoon highs for 2 or three days. It may just be that mycelium can't take the heat here in summer. I am getting a little tired of it my own self. 82f(28c) here today for a new record.

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Ah, gotcha!
Duely noted in that case

ReverendMyc said:

fahtster said:

ReverendMyc said:

I don't know if it is heat or dryness here, but I have struggled to get any recovery from dried grains that have been sent to me. I will toss a couple of ones I did my self a while back on some plates to see if I can rule out dryness.

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They need to be cut off from drying at some point.. when I have them just sitting in a jar with large open filters, they tend to die ~12-18 months.  When I put them in a sealed container, they have lasted 2+ years.. I haven’t run into the limit yet.  Good rule of thumb is that if the starch inside the grain has dried and turned to dust/emptied, the myc is dead

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Good info. Thanks fahtster.
I put some PE and semps grains into cryo tubes in April 2022 after drying in the jar for a month or so. I will put them to agar next time I start up the ffu. Looking up those dates I discovered that the PE was the culture that produced my largest blob ever in a Faht-loc bag. I would sure love to do that again.

It would be really useful for dehydration and storage methods vs potency testing.

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Over 2.5 year old PE on dried wheat grains starting to recover after 6 days on agar. Pretty stoked to see that this is an option that does work.

ReverendMyc said:

ReverendMyc said:

fahtster said:

ReverendMyc said:

I don't know if it is heat or dryness here, but I have struggled to get any recovery from dried grains that have been sent to me. I will toss a couple of ones I did my self a while back on some plates to see if I can rule out dryness.

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They need to be cut off from drying at some point.. when I have them just sitting in a jar with large open filters, they tend to die ~12-18 months.  When I put them in a sealed container, they have lasted 2+ years.. I haven’t run into the limit yet.  Good rule of thumb is that if the starch inside the grain has dried and turned to dust/emptied, the myc is dead

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Good info. Thanks fahtster.
I put some PE and semps grains into cryo tubes in April 2022 after drying in the jar for a month or so. I will put them to agar next time I start up the ffu. Looking up those dates I discovered that the PE was the culture that produced my largest blob ever in a Faht-loc bag. I would sure love to do that again.

It would be really useful for dehydration and storage methods vs potency testing.

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Over 2.5 year old PE on dried wheat grains starting to recover after 6 days on agar. Pretty stoked to see that this is an option that does work.

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Final update on those dried grains. After 2.5+ years, they all grew out to this in 15 days. Already have transfers of the best looking spots.

Bigworm said:

Thanks for the info. I'll be switching to lower nutes for my next round of plates. I think I'm going to try to dry some grains when I spawn my next jars in a day or two.

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3g lme
8g agar
500mL h2o

my go-to recipe for low nute plates

Scemilope said:

True Albino Teacher revived from a 2.5+ year old distilled water stasis vial.

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