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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: what do do with colonized plates?? [Re: LewDoja] * 4
    #29050752 -



First foray into SSLC... Free poured on 12-4 how's it looking?

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Re: what do do with colonized plates?? [Re: Pnin]
    #29050876 -

lookin great from here

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Re: Re: AGAR ENVY! (Anything and All things agar!) [Re: LewDoja]
    #29050898 -

FLHX said:
I’ve just recently started working with agar....




LewDoja said:
Quote:
I’ve just recently started working with agar


..shuuuure ya did...

Nice looking plates for "just starting".

from now on your the noob agar ambassador.

...


FLHX please don't change the title of long-running main threads here.

I've changed it back.

LewDoja, do you think FLHX is lying about just starting plates? Or is that a compliment to him that I didn't catch?

He's talked with me some and is certainly asking the right questions for a curious noob who wants to be a quick learner.

He does have great looking plates, even not for a noob, but it's possible. Some people just get shit right, especially people who follow directions without "improvising" the first time like many people.

I've noticed this a lot with engineers. (I don't know that he is one though)

For example, a person who's trained as a software engineer or electrical engineer can sometimes do a project and do every aspect of it nicely the first time.

Like designing and building a computer-driven device from scratch, then building a nice case for it, doing nice lettering on it, writing a good manual for it, etc

As for plates in fridge, I've revitalized one after 11 months. Just stored in a zip lock baggie, upside down, so water doesn't condense on the agar, and kept the fridge just above freezing. Checked in summer, had to turn the fridge down a tiny bit to keep at 34F.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (12/09/24 01:21 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 5
    #29050956 -

Locked the subject line:super:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cronicr]
    #29050983 -

cronicr said:
Locked the subject line:super:


Thank you.

I didn't know Mods could do that.

I'd recommend also locking
"Post Your Cultivation Picture of The Day"

That one gets hijacked sometimes.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 13
    #29051011 -


200 autoclavable 60 ml dishes , they didn't have the 90 but that's fine


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cronicr]
    #29051078 -

cronicr said:

200 autoclavable 60 ml dishes , they didn't have the 90 but that's fine


Hell yeah. Im going for the same as soon as the wallet allows!


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ThurmanMurman] * 1
    #29051162 -

I paid like $40 for 400...

Good addition,  just not a complete substitute for 90s.


@nektar

It was sarcastically worded compliment.

If you're the driving force behind their results- :congrats: on you.

I love seeing newly registered members achieving birdies/eagles (above par, although technically sub-par is better in golf scoring).

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LewDoja] * 2
    #29051179 -

I must have developed a special talent to transform rhizo growth into tomentose at this point :rofl:
Some x7x+ T2 that look healthy but tomentose.



I will start trying agar recipes with 3-4% brf as nutes since going 2% and lower only gave me weaker growth. At 0.5% the myc was almost invisible. I’m also going to start using a new agar supplier instead of the “dessert/food” agar I used in previous batches so maybe that helps as well.

I just hate that the same myc looks beautifully rhizo in brf pucks and grain but shit in agar.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LewDoja]
    #29051183 -

LewDoja said:
I paid like $40 for 400...

Good addition,  just not a complete substitute for 90s.


@nektar

It was sarcastically worded compliment.

If you're the driving force behind their results- :congrats: on you.

I love seeing newly registered members achieving birdies/eagles (above par, although technically sub-par is better in golf scoring).


Thats a hell of a deal! I think it's $89 for a 100pk but I think those were 90mm.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ThurmanMurman] * 1
    #29051258 -

I just noticed he said autoclavable,
To which mine unfortunately are not.

60s seem like they'd be good for that.  Just think all of things you'd be able to aquire to hold them.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ThurmanMurman]
    #29051265 -

ThurmanMurman said:
LewDoja said:
I paid like $40 for 400...

Good addition,  just not a complete substitute for 90s.


@nektar

It was sarcastically worded compliment.

If you're the driving force behind their results- :congrats: on you.

I love seeing newly registered members achieving birdies/eagles (above par, although technically sub-par is better in golf scoring).


Thats a hell of a deal! I think it's $89 for a 100pk but I think those were 90mm.


cronicr said:

200 autoclavable 60 ml dishes , they didn't have the 90 but that's fine



99 dollars for 500 sterile petris:
https://www.southernlabware.com/petri-dish-polystyrene-90-x-15mm-sterile-500-cs.html
I don't think they can be autoclaved, but I PC agar mix then pour plates.

It says 100 mm but they sent out 90 mm in my order this year, still fine. Too much hassle to return and I don't care.

Pic tax, a little rhizo growing on x7x+ jar of birdseed. I tried to post with the jars earlier but there was an issue uploading here for some reason.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (12/09/24 08:31 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #29051288 -

Not bad.  I do prefer the 10 count bags over 20. For large plates at least.

Though you guys be working in the flow.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator] * 1
    #29051301 -

AspectOfTheCreator said:


Took some early transfers from this but after growing out this plate looks the best and appears clean. Is it safe to put to grain?



I would send it to grain.
:awesomenod:


--------------------

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #29051374 -

nektar61 said:
ThurmanMurman said:
LewDoja said:
I paid like $40 for 400...

Good addition,  just not a complete substitute for 90s.


@nektar

It was sarcastically worded compliment.

If you're the driving force behind their results- :congrats: on you.

I love seeing newly registered members achieving birdies/eagles (above par, although technically sub-par is better in golf scoring).


Thats a hell of a deal! I think it's $89 for a 100pk but I think those were 90mm.


cronicr said:

200 autoclavable 60 ml dishes , they didn't have the 90 but that's fine



99 dollars for 500 sterile petris:
https://www.southernlabware.com/petri-dish-polystyrene-90-x-15mm-sterile-500-cs.html
I don't think they can be autoclaved, but I PC agar mix then pour plates.

It says 100 mm but they sent out 90 mm in my order this year, still fine. Too much hassle to return and I don't care.

Pic tax, a little rhizo growing on x7x+ jar of birdseed. I tried to post with the jars earlier but there was an issue uploading here for some reason.



I'm canadian that's like 400 bucks 😂
I don't really need much just enough to get clean growth for lc , I do agar to lc to bags


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 3
    #29051460 -

nektar61 said:
FLHX said:
I’ve just recently started working with agar....




LewDoja said:
Quote:
I’ve just recently started working with agar


..shuuuure ya did...

Nice looking plates for "just starting".

from now on your the noob agar ambassador.

...


FLHX please don't change the title of long-running main threads here.

I've changed it back.

LewDoja, do you think FLHX is lying about just starting plates? Or is that a compliment to him that I didn't catch?

He's talked with me some and is certainly asking the right questions for a curious noob who wants to be a quick learner.

He does have great looking plates, even not for a noob, but it's possible. Some people just get shit right, especially people who follow directions without "improvising" the first time like many people.

I've noticed this a lot with engineers. (I don't know that he is one though)



I didn’t realize that would change the title of the thread. I’m sorry for doing that. It was unintentional. I know now. Won’t happen again. Lesson learned.

As far as the plates go I recently had to start from scratch.
I was using LC and didn’t realize it was all contaminated so I switched to agar. I had to throw everything out and start from scratch so I shopped some new cultures and got them as research plates. The plates I got in the mail were gorgeous.

When I received the plates, I made transfers so I could grow some new plates to use to knock up some grain spawn jars.

The pictures are the first plates I poured myself and transferred from the new shopped plate. So the rhyzomorphic growth was already like that on the original plate.
I thought they looked really good too. Not bad for the first time I did a transfer, but the culture had already been….  Not sure the right term for it….  Nurtured or isolated to have the spoke wheel looking mycelium.
I think your comment about me possibly lying is because they look really cool, right?  It’s because the original plates I took a transfer from had that wagon wheel spoked look to it as well.

It was never my intention to pass the plates off as a culture I isolated and nurtured myself. It it comes across that way it is unintentional. I just wanted to show what I meant by it being fully colonized. The credit should go to the vendor the plates came from.

I’m still too new to get results like that on my own, like getting a culture to go from tomentose growth to rhyzo growth.
I posted pictures because I wasn’t sure about storage. That should tell you all you need to know about my level of experience.


I hope that clears things up.
The last thing I wanna do is pull some sketchy shit like take credit for something I didn’t do. This site has been invaluable to me. It’s important that I maintain a respectable and honest reputation since I plan on utilizing this resource as heavily in the future as I do now, and because that’s the way you’re supposed to act anyways.

If I made some sort of breach of etiquette by not explaining that when I originally posted the pictures, it was unintentional.  I wasn’t aware.


--------------------
Only those who attempt the absurd will achieve the impossible. I think it's in my basement... let me go upstairs and check.

-M. C. Escher

Edited by FLHX (12/11/24 03:13 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: FLHX] * 5
    #29051817 -

You are good my friend.

Changing the the subject and getting called out on it is practically a right of passage here. It is an easy mistake to make and not really obvious until someone notices and says something. The Moderators and the person who creates a thread can lock the subject, but I think that is a new(er) feature so it has not been fully implemented everywhere. The Cronic-meister has locked it for this thread now.

I am pretty certain that the Lew Ninja was just admiring your prodigy level start at agar. Even from LC it take some skills and patience to learn everything you need know to get to plates as pretty as yours. Good on you for taking the time to learn and do it right from the start.

Please do edit your post above to remove vendor information, though. Our sponsors support the site and so we don't want them to get in trouble for selling anything for non-microscopy or non gourmet growing related. And we don't want to promote non sponsors when the reason this place exists to help is due to the support of the Trusted Vendors.

This is a great community with amazing people, like nektar, always willing to help. Sometimes the tone and humor is hard to get over text, but most stuff you will see in cultivation is written with the best of intentions.

Except piggy, that one is a jerk. JK. Love you piggy.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

Edited by ReverendMyc (12/10/24 10:35 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] * 7
    #29051823 -

Autism baby, there ain't any emotion in those facts.

:fingerpistol:


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr Piggy] * 8
    #29051832 -



Pa. cyanescens, Tea Tree, South Africa

Looking spicy! 🥰

Edit: sequencing has not been completed.

Edited by PhenoDreamers (12/12/24 07:17 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] * 2
    #29051841 -

ReverendMyc said:
Except piggy, that one is a jerk.



thanks for the warning :lol:


--------------------
Learn it, know it, live it


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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