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Re: AGAR ENVY! (Anything and All things agar!) [Re: HILLBILLY OUTLAW] * 1
    #28343185 -

HILLBILLY OUTLAW said:
sprocket said:
Thank you! I was hoping that was the situation. Is it safe to assume the ones that devour the coloring are the mose vigorous colonies?



I’m not positive on that aspect. Could be the case.
:justdontknow:



I can do a side by side with transfers from plates that are clear and one with color still in it. See how they go.


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How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket] * 2
    #28343186 -

sprocket said:
Or both :grin:  I made BRF jars today so they'll be ready to inoculate tomorrow. Think I can get 3 quarts from it or am I getting greedy.



You can do like 8 quarts+ with just the plate, or take a wedge and expand to LC and inoculate as many as you want. Or inoculate 1 wbs jar, let that colonize, and g2g like 12 off a single wedge.

Expansion is a game of “how much would you like,” not “how much can you do.”

A small piece from that dish could make a gallon of LC if you had the space and equipment.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket] * 1
    #28343189 -

sprocket said:
I made BRF jars today so they'll be ready to inoculate tomorrow.



Me too! I am running GT this time.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yahra]
    #28343192 -

Actually, I did knock up a LC jar today with the same APE6, just from a different plate.  All my APE6 plates are from one clone I took months ago.


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket]
    #28343193 -

The other day I didn't wait long enough for some freshly poured plates to cool and solidify, and noticed that the softness of the receiving agar is inversely proportional to the quality of the myc growth.

It goes like this:

1st transfer, transfer wedge got almost entirely buried in the agar. Atrocious growth.
2nd transfer, wedge stuck half into the agar. Bad growth.
3rd transfer, wedge a little bit submerged. So-so growth.
4th transfer, I let the plates cool completely. Normal growth.

I knew that myc grows much better and faster on the surface of the agar rather than through it, but I thought it would still manage to grow. Well I guess not.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 5
    #28343196 -

You should let your plates cool down to room temp before using them my friend.


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: HILLBILLY OUTLAW]
    #28343197 -

:whathesaid:


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket] * 4
    #28343200 -

Patience is crucial in this hobby. God knows it's hard to wait, but wait you must. That's why I call it cultiwaiting


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket]
    #28343201 -

haha it wasn't on purpose, I thought they were done but they weren't. :shrug:

Gotta fuck up to learn. :rollsafe:


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: altford78] * 1
    #28343203 -

If that's the case, then I am learning the shit out of this hobby lol.


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28343207 -

:curbyourenthusiasm:


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket]
    #28343210 -

sprocket said:
Actually, I did knock up a LC jar today with the same APE6, just from a different plate.  All my APE6 plates are from one clone I took months ago.



You’re keeping the first plate with the tissue sample though yeah?


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28343214 -

Unfortunately that plate contaminated some time ago. Guess I took one too many transfers from it, but once I get a flush from these, I'll get another clone. Maybe do a slant to keep it around. It's a game now to see how long I can keep it going.


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket] * 1
    #28343217 -

Ah gotcha. Bummer. I try not to transfer away from clones anymore. Lost phenotypes without ever having the opportunity to see if I saved them in the first place because I straight up transferred to like T6 before running the tissue clone.

But there’s always more shrooms to save.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28343226 -

Definitely! It would be nice to say hey remember that LC syringe I bought 5 years ago? Yeah when it's still going strong! That's getting your money's worth


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28343251 -

I've been wanting to post this for a while but haven't had time to sit down and go through it.
got some genetics from a buddy of mine, the cups below are both t2, ape & hp, in order.



I have been seeing weak, tomentose growth on alot of plates lately. Tried switching the agar recipe to see if I was missing anything.
These plates are:

500ml city tap
10g Briess LME
10g Fitlane agar-agar

Someone told me grain water can cause wispy uncertain growth like this, but I find these ape cups especially disconcerting and foul-looking for cube myc, so that was ousted.
Pictured next to it is hillbilly pumpkin, which doesn't look great either, but at least i see some willingness there.
I strongly feel it's not contaminants or bacteria, because I inoculated 10 of each at the same time, same SAB, same tools, within 30min. Every single ape cup looks exactly like the picture. Every single hillbilly cup looks exactly like that picture, no visible contam except for every ape cup looking like mold.
I would normally say "hey that looks like mold" if someone posted this, but I'm really confident it's a combination of genetics and conditions (including agar recipe)
When put to grain I firmly believe it will express totally different, as cube myc do.
Is the minerality or treatment of the water source at play here?
I am thinking about using RO or distilled water, in that order to see what works.
What do you guys think?


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----<><><>----
tail-lights, back-pockets, rearview mirrors

guides | grows | supreme air box | sporeaddict
bring back ῥͳϻ

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Adas]
    #28343334 -

Adas said:
RoscoeReturns said:
Screwup said:
RoscoeReturns said:
Let the battle begin!





What the fuck is that black Shit never seen something like that lol



Don’t know what it is, but it was on my fingers. Kind of afraid for whatever I touched after that.



Based on the color I'd say it could be a Pseudomonas. The same stuff that forms this black biofilm on wet surfaces (like your shower).



That’s not good. I work at a hospital, so it’s likely multi drug resistant. I’m growing super bugs!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28343357 -

You probably brought some MRSA home from that hospital.

:disco:


--------------------

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: HILLBILLY OUTLAW]
    #28343375 -

You got the ghonocacacaus!! What ever you do, don't touch your dick, it'll end up looking like a burnt match!!


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket]
    #28343462 -

sprocket said:
You got the ghonocacacaus!! What ever you do, don't touch your dick, it'll end up looking like a burnt match!!



You’re assuming it doesn’t already.

Why does it hurt when I pee?

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   Myyco.com APE Liquid Culture For Sale   North Spore Cultivation Supplies


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