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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: uninc4life2010] * 1
    #28212694 -


Okay, so this is 1/3 that came out and from what I underatand it looks good. But you never know. Can anyone tell me if it looks clean and good? I would like to make another transfer tomorrow ann any suggestions would be appreciated as I'm a total newbie. It's mexican spores and I would like to know if anyone can see any contamination or know how many more transfers I need?


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Understanding is enlightening, always be open to fresh input and diversity! Be a better man respect others as you would like to be respected.


"The greatest journey of exploration is to understand our own minds. #Exploration #Understanding #Mind

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shromolo] * 1
    #28212729 -

Shromolo said:

Okay, so this is 1/3 that came out and from what I underatand it looks good. But you never know. Can anyone tell me if it looks clean and good? I would like to make another transfer tomorrow ann any suggestions would be appreciated as I'm a total newbie. It's mexican spores and I would like to know if anyone can see any contamination or know how many more transfers I need?


What do you mean by "this is 1/3 that came out"?  BTW, it's totally clean.  I would take a transfer from the 2 O' clock through the 4 O' Clock position or the 7 O' clock through the 12 O' Clock position.  Or both.


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https://www.shroomery.org/forums/showthreaded.php/Number/22158748/page/0/vc/1

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycovester]
    #28212734 -

Mycovester said:
Thank you. That's amazing.

Does it colonise the at the same speed as agar wedged into grain?


Depends on how much agar you use. Gram for gram itā€™s about the same speed as agar, maybe a bit slower since the pin has to revert to mycelium first.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: uninc4life2010] * 1
    #28212804 -

uninc4life2010 said:
How's this clone culture looking?  DAE rarely have to perform a second transfer to clean up agar plates?  I have about 10 T1 plates that came from a couple of clone cultures, and not a single one appears to have contams.



Looks ok, is that satellite @12oclock from dropped myc?


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You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cozmyc] * 1
    #28212864 -

cozmyc said:
uninc4life2010 said:
How's this clone culture looking?  DAE rarely have to perform a second transfer to clean up agar plates?  I have about 10 T1 plates that came from a couple of clone cultures, and not a single one appears to have contams.



Looks ok, is that satellite @12oclock from dropped myc?


It's like a blob of agar that formed in a little round mound.  It's def not a bacterial colony or a mycelial growth spot.


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https://www.shroomery.org/forums/showthreaded.php/Number/22158748/page/0/vc/1

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Re: AGAR ENVY! (Anything and All things agar!) [Re: uninc4life2010] * 3
    #28213358 -

Enigma T1s this evening; took six T2s:


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Seekerā€™s LAGM 2023 Journal
Jake Oncidā€™s Tek for Panaeolus | Official Ps. Zapotecorum Thread | Moober's Notes on Growing Natalensis | Muad.Dweebā€™s Breeding Resources

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Seeker604]
    #28213455 -

Question.  How careful are you about getting iso onto your plate when transferring agar to agar?

I use quite a bit spraying my surfaces and hands and inside my SAB, I often wonder if a drop would kill a plate? 5 drops? Just the vapors? I guess i could test it but first I thought I'd ask if this is a common question here.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DreadedFarms] * 1
    #28213458 -

It is not advisable to drip any amount of alcohol into any plate for any reason. Just let it vape off first. The vapors won't hurt anything.

Hope that helps.


--------------------
Question everything, it's very responsive.
culture storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl]
    #28213500 -

San Pedro Girl said:
Mycovester said:
Thank you. That's amazing.

Does it colonise the at the same speed as agar wedged into grain?


Depends on how much agar you use. Gram for gram itā€™s about the same speed as agar, maybe a bit slower since the pin has to revert to mycelium first.


Thank you so much


--------------------


I'm half pencil half mushroom.

It's great.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycovester] * 3
    #28213999 -



Oh lala!


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I'm half pencil half mushroom.

It's great.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycovester] * 1
    #28214089 -

.


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

Edited by Eugene Gesuale (03/12/23 07:57 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DreadedFarms]
    #28214100 -

DreadedFarms said:
Question.  How careful are you about getting iso onto your plate when transferring agar to agar?

I use quite a bit spraying my surfaces and hands and inside my SAB, I often wonder if a drop would kill a plate? 5 drops? Just the vapors? I guess i could test it but first I thought I'd ask if this is a common question here.


Well, considering the properties of isopropyl, I would definitely be careful to not get any on your agar or mycelium. I don't use an SAB, I work in front of a flow hood and I spray everything down before starting my transfers, but I allow the outsides of the petris, bags, scalpels, etc. to fully dry/evaporate before I expose any sterile cultures.

Hope that helps!


--------------------


Seekerā€™s LAGM 2023 Journal
Jake Oncidā€™s Tek for Panaeolus | Official Ps. Zapotecorum Thread | Moober's Notes on Growing Natalensis | Muad.Dweebā€™s Breeding Resources

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycovester]
    #28214123 -

Mycovester said:


Oh lala!


:bender:


--------------------

Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28214311 -

Blurry photo of last week's plin clones to be transferred soon, and some rhizo jar x7x.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28214355 -



This about 10 days along, cleaning up some messy spores.  Does this look good for keeping near the 12 to 3 o'clock? APE revert from an undisclosed supplier in Canada.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DreadedFarms] * 1
    #28214356 -

DreadedFarms said:


This about 10 days along, cleaning up some messy spores.  Does this look good for keeping near the 12 to 3 o'clock?


Yes. That edge looks good to transfer from.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 4
    #28216217 -

Here is the same agar plate two days apart.  I think it'll be ready to go to grain within the next 5-7 days.



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https://www.shroomery.org/forums/showthreaded.php/Number/22158748/page/0/vc/1

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Re: AGAR ENVY! (Anything and All things agar!) [Re: uninc4life2010] * 5
    #28217099 -

Melmac clone plate


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Whyterye] * 2
    #28217128 -

:idhitit:


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycovester] * 1
    #28217532 -

Mycovester said:
Mycovester said:
https://64.media.tumblr.com/d657a4365f3e842042871ba119b7ed40/7f5679f3135e7655-c9/s2048x3072/cae3113cd06e4044f1b1a408c9170835afceae16.jpg

Beautiful contamination.

I wish I could say I planned this. I wish I knew how I managed it!

Mesmerising




I can post pics! Woopeeee

This is the plate I was trying to share.

I have no idea how I managed it.


That is gorgeous actually. It doesnt even look like mold. I hope you kept it.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
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Azurescens in June!

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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