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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shrimps] * 2
    #28159828 -

*New Guy* If that's not obvious... any ways I have several I'm working with I'm trying to learn from. I'll love any constructive criticism given. I expect the worst.


this is "stargazer" I received as an Isolated Spore Syringe I grew out on agar then tried to isolate Rhizomorphic growth and ended up with this. i noticed the stargazer always seems so look almost "spotted"?



here is more attempts at isolating B+ rhizo. I have a plate of where I took it from. It seemed like the more I tried to isolate this strain the less Rhizo would pop up....Maybe it's watering out the genes????


This is me testing out an LC I made out of the stargazer ISS to test for contamination before innoc. millet jars.



This right here\/ I'm very proud of "mexi" i germed from spore print this is the second transfer and it's trying to reach out for the heavens but I see contamination and sectoring.. I didn't know if this is going to effect me trying to grow this plate out for later inoculation????


This last one I think is the ugliest thing ever. With that being said. I had a healthy ape jar I was spawning and thought "Oh I should grab some genes before I throw it in the sub. So I put the grain on the plate in the SAB but it was not center at all. So I played this stupid unintentional game of roll the grain on the plate for a while. I'm just trying to keep the genetics going on... it looks like mycelium is eating most everything else. Do you think it'll grow out or trash it?????




really, thank you for any educational responses in advance.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Milonaire Mycology]
    #28160173 -

I'm using 1/2 pint jars with unmodded lids for agar. Do I do the same as with grains? In other words, should I flip the lid and leave slightly untightened for GE? Or can I put the lid on the "right" way and seal tight with no holes? Not sure how much GE (if any) agar plates need.


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Edited by AspectOfTheCreator (01/27/23 04:46 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28160189 -

They need oxygen, but should have enough in the jar with the lids tightened down.


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Mold farming ain't much, but it's honest work.

FlawdaKraka's LAGM 2.023

Clean culture
Sterile prepped grains
Properly hydrated coir
Maintaining Surface Conditions
Fungus will do the rest


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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28160203 -

AspectOfTheCreator said:
I'm using 1/2 pint jars with unmodded lids for agar. Do I do the same as with grains? In other words, should I flip the lid and leave slightly untightened for GE? Or can I put the lid on the "right" way and seal tight with no holes? Not sure how much GE (if any) agar plates need.


What kind of lids? Unless you use silicone gaskets, they wonโ€™t be air tight, even tightened down.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl]
    #28160210 -

i dunno pedro, rumfor69 was just talkin to me about some gray canning lids that have no gasket and apparently seal
up nice? he might wanna see this discussion before he runs a shit load of em


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Need a lift?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme]
    #28160235 -

I dig those green plates! makes me want to try it. what dye do you use?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme]
    #28160245 -

BeefSupremeJr said:
i dunno pedro, rumfor69 was just talkin to me about some gray canning lids that have no gasket and apparently seal
up nice? he might wanna see this discussion before he runs a shit load of em


I guess it depends on what you call a seal. I mean that there will still be some gas exchange for O2 in the same way that plastic wrap around agar plates still allows gas exchange like Parafilm so the seal isnโ€™t good enough to stop mycelium on agar or prevent it from pinning, etc.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme]
    #28160266 -

These lids?



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Re: AGAR ENVY! (Anything and All things agar!) [Re: donrjuan] * 4
    #28160290 -



Saw someone mention reviving dry mushrooms. I wanted to try, so scraped some gill tissue from dried Averyโ€™s fruit onto a couple agar plates. Lotโ€™s of growth in just a few days. I donโ€™t know if itโ€™s just spores germinating, or if the dry tissue was resurrected.


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Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28160312 -

Looks like germ plate imo. Prob a mix of both ms and cln growth.


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๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: HILLBILLY OUTLAW] * 2
    #28160329 -

ive cracked a capsule of ksss someone gave me open and put a bit on a plate.  made a real
mess but i eventually got a clean culture from it.  it was good experience.


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Need a lift?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: donrjuan]
    #28160398 -

donrjuan said:
These lids?





Those lids are air tight if you use the silicone gasket they come with.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl] * 1
    #28160403 -

Those don't even come with silicone gaskets, at least not the box I bought.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28160411 -

SirPsycho said:
Bobgas said:
Is there anything suspect on this plate?  It's clone tissue.  I am wondering if I can take 2-6 and 7-9 to grain.  It's my first clone to agar.




The centre look like a vagina.

Nice.

:thumbsup:


HAHAHAHA!!  I think the same thing each time I look at it.

Thank you Shrimps!

I learned tonight that being in a rush and doing agar work is not a good idea.

I am headed away for a week and wanted to get grain started and do transfers of HBOโ€™s Ghost and GT plates that I have.  I am fucking everything up and there is no time for a redo.

O well.  Hopefully the plates dont get whacky in a week in the fridge.


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๐Ÿ…ƒ๐Ÿ„ด๐Ÿ„ฐ๐Ÿ„ผ  ๐Ÿ„ฒ๐Ÿ„ป๐Ÿ„ธ๐Ÿ„ฝ๐Ÿ„ถ๐Ÿ…†๐Ÿ…๐Ÿ„ฐ๐Ÿ„ฟ

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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl]
    #28160467 -

San Pedro Girl said:
AspectOfTheCreator said:
I'm using 1/2 pint jars with unmodded lids for agar. Do I do the same as with grains? In other words, should I flip the lid and leave slightly untightened for GE? Or can I put the lid on the "right" way and seal tight with no holes? Not sure how much GE (if any) agar plates need.


What kind of lids? Unless you use silicone gaskets, they wonโ€™t be air tight, even tightened down.


The regular lids that come with ball mason jars with the rubber perimeter. I assure you they're air tight. Why else do you think we crack the lids when PCing? If you don't think so go seal one up and PC it. LMK how it goes.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28160483 -

I think she was assuming you were using one-piece plastic lids. Those are commonly used and highly recommended over the two-piece metal lids.

With that being said, I really dislike using jars for agar, but they can work. Mainly, I just didn't like the steep angle it forces me to use for transfers. That makes it difficult to get the agar segment placed in the middle instead of stuck on the tip of the scalpel or knife.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl]
    #28160577 -

San Pedro Girl said:
BeefSupremeJr said:
i dunno pedro, rumfor69 was just talkin to me about some gray canning lids that have no gasket and apparently seal
up nice? he might wanna see this discussion before he runs a shit load of em


I guess it depends on what you call a seal. I mean that there will still be some gas exchange for O2 in the same way that plastic wrap around agar plates still allows gas exchange like Parafilm so the seal isnโ€™t good enough to stop mycelium on agar or prevent it from pinning, etc.


Yeah the plastic Ball brand lids have a plastic bevel in them that works how the lid on a pyrex media bottle does. I put water in a jar screwed a lid down tight shook shaked jiggled for a bunch of mins not a drop came out and after opening it was dry beyond the bevel. Guess I'd say good to go? :shrug:


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Whyterye] * 1
    #28160624 -

I used three drops of food coloring i wish i would have only used 2 or 1. Less is better for sure.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Milonaire Mycology] * 2
    #28160634 -


Ive been running into a rash of penicillium (white with green center appearing later), but I think Ive finally gotten a clean culture of something beside cubes.

Im experimenting with using less agar in my recipe for a softer result as I was having trouble getting spores to germinate, and so far the myc growth looks better in general on these plates, even though they look kind of unclear. Potentially my LME is caramelizing?

Edited by shitakee (01/27/23 11:07 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: shitakee] * 1
    #28160657 -

Are you doing pour or no-pour? I doubt it's caramelizing, as I've ran no-pour MEA for 45 minutes at 15 psi and they are still clear. What recipe or percentages are you using?

I'm a fan of softer agar. I think it should be as soft as possible and still be transferrable, and not only for germ plates either.

That transfer is looking good though!

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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