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 PsiloPsychIn
PsiloPsychIn
 
Registered: 06/17/14
Posts: 8,182
Loc: up north
Last seen: 1 month, 29 days
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 Re: AGAR ENVY! (Anything and All things agar!)  [Re: SirPsycho]
#28162646 - 01/29/23 10:25 AM (1 year, 2 months ago) |
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Am I the only clumsy idiot who has ever knocked a stack of freshly poured plates into his flowhood? 🤪
If not … how did you clean the agar off your filter? The damage in this case is only an area of about 2x3 inches…
I’m thinking I will get some near boiling hot water in a spray bottle and try to spritz it way. Maybe follow with a solid dousing of Iso. Maybe a good spray of Lysol to seal the deal. I definitely don’t want to scrape or scrub on the filter.
Thoughts?
Some agar problems are hard…
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What are they saying? Listen carefully, it might be something you need to hear... 
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 bakedbeings
orbiter of truth
Registered: 09/01/20
Posts: 4,218
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Re: AGAR ENVY! (Anything and All things agar!) [Re: PsiloPsychIn]
#28162647 - 01/29/23 10:26 AM (1 year, 2 months ago) |
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mushboy did somethink like that i think
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🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
   
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Shrimps
Traveler
Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
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Re: AGAR ENVY! (Anything and All things agar!) [Re: PsiloPsychIn]
#28162650 - 01/29/23 10:26 AM (1 year, 2 months ago) |
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I dig the lavender color
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JW123
Nooby Nooby Noob
Registered: 01/17/22
Posts: 1,383
Loc: Tn
Last seen: 8 hours, 6 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
#28163483 - 01/29/23 08:16 PM (1 year, 2 months ago) |
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Damn. I always thought that was lion's mane. Beautiful.
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Scemilope
Mycoheretic
Registered: 11/16/22
Posts: 634
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Re: AGAR ENVY! (Anything and All things agar!) [Re: JW123] 5
#28164087 - 01/30/23 09:57 AM (1 year, 2 months ago) |
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A clone is from this batch of B+.
They were thicker and had wavier caps than the other 3 tubs that round.
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This hobby has taught me many things. Most of those things are patience.
Psily-Shots
Hobbit’s Secondhand Mycology Challenge
Edited by Scemilope (01/30/23 09:58 AM)
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ruawakeyet
Registered: 05/03/21
Posts: 1,862
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Scemilope] 1
#28164091 - 01/30/23 10:02 AM (1 year, 2 months ago) |
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And apparently that round of B+ went albino...
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Scemilope
Mycoheretic
Registered: 11/16/22
Posts: 634
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ruawakeyet] 2
#28164108 - 01/30/23 10:17 AM (1 year, 2 months ago) |
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Quote:
ruawakeyet said:
And apparently that round of B+ went albino...
😂 It's from an overnight time lapse. I was so excited to harvest I forgot to get pictures.
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This hobby has taught me many things. Most of those things are patience.
Psily-Shots
Hobbit’s Secondhand Mycology Challenge
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ruawakeyet
Registered: 05/03/21
Posts: 1,862
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Scemilope] 1
#28164119 - 01/30/23 10:25 AM (1 year, 2 months ago) |
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Oh, I see now. Thanks for explaining!
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San Pedro Girl
Shoebox Ninja🥷
Registered: 07/17/12
Posts: 2,447
Loc: Fuck off pig!🐷
Last seen: 27 days, 21 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ruawakeyet] 7
#28164422 - 01/30/23 01:46 PM (1 year, 2 months ago) |
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First time with APE. I hope one of these plates germinate.
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Guerrilla
Bumbaclart
Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom 
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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl]
#28164425 - 01/30/23 01:49 PM (1 year, 2 months ago) |
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Good luck! ♡
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Being pissed on does not make you a real man.
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Keefersutherland
Strange
Registered: 09/02/20
Posts: 154
Loc: Upstate NY
Last seen: 11 months, 22 days
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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl]
#28164496 - 01/30/23 02:51 PM (1 year, 2 months ago) |
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Quote:
San Pedro Girl said:
First time with APE. I hope one of these plates germinate.
I’ve never gotten APE to germ on my streak plates. I always have to jamb the swab in the agar and it germinates around that area.
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LAGM 2023
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San Pedro Girl
Shoebox Ninja🥷
Registered: 07/17/12
Posts: 2,447
Loc: Fuck off pig!🐷
Last seen: 27 days, 21 hours
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Quote:
Keefersutherland said:
Quote:
San Pedro Girl said:
First time with APE. I hope one of these plates germinate.
I’ve never gotten APE to germ on my streak plates. I always have to jamb the swab in the agar and it germinates around that area.
I figure I’ll give it a couple weeks. Then I’ll put a couple drops of sterile water on the swabs and streaks and give it another couple weeks. After that I’ll be begging for someone to send me more swabs.😂
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Guerrilla
Bumbaclart
Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom
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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl] 1
#28164580 - 01/30/23 03:46 PM (1 year, 2 months ago) |
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Would have had a higher probability of success by pulling cotton filaments with your scalpel and burying those into multiple plates  but fingers crossed.
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Being pissed on does not make you a real man.
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milkboy
Child
Registered: 05/01/21
Posts: 2,347
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
#28164707 - 01/30/23 04:55 PM (1 year, 2 months ago) |
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I once had a APE streak plate germinate after 5 weeks. It can happen
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Gummies by milkboy
Milkboys Public Display of Affection
When the student is ready, the teacher appears
   
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San Pedro Girl
Shoebox Ninja🥷
Registered: 07/17/12
Posts: 2,447
Loc: Fuck off pig!🐷
Last seen: 27 days, 21 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla] 1
#28164716 - 01/30/23 05:03 PM (1 year, 2 months ago) |
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Quote:
Guerrilla said:
Would have had a higher probability of success by pulling cotton filaments with your scalpel and burying those into multiple plates but fingers crossed.
Thanks for the tip! I’ll try that.
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Guerrilla
Bumbaclart
Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom
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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl] 4
#28164726 - 01/30/23 05:09 PM (1 year, 2 months ago) |
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Quote:
San Pedro Girl said:
Quote:
Guerrilla said:
Would have had a higher probability of success by pulling cotton filaments with your scalpel and burying those into multiple plates but fingers crossed.
Thanks for the tip! I’ll try that.
 I do recommend dipping the swab in sterile water first though. It makes the cotton much easier to separate and hydrates spores too.
I use 8oz jars to PC my water but no pour containers would work too.
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Being pissed on does not make you a real man.
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San Pedro Girl
Shoebox Ninja🥷
Registered: 07/17/12
Posts: 2,447
Loc: Fuck off pig!🐷
Last seen: 27 days, 21 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
#28165591 - 01/31/23 08:11 AM (1 year, 2 months ago) |
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Quote:
Guerrilla said:
Quote:
San Pedro Girl said:
Quote:
Guerrilla said:
Would have had a higher probability of success by pulling cotton filaments with your scalpel and burying those into multiple plates but fingers crossed.
Thanks for the tip! I’ll try that.
I do recommend dipping the swab in sterile water first though. It makes the cotton much easier to separate and hydrates spores too.
I use 8oz jars to PC my water but no pour containers would work too.
Since it was just sitting in the lab fridge, I diluted some leftover agar. It’s in the PC now. I figure dipping the swabs in sterile nutrient juice before shredding on the plate is superior to just water alone. Thanks again.
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Grogu
Registered: 07/02/22
Posts: 1,712
Last seen: 10 months, 28 days
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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl] 1
#28165617 - 01/31/23 08:23 AM (1 year, 2 months ago) |
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Quote:
San Pedro Girl said:
Quote:
Guerrilla said:
Quote:
San Pedro Girl said:
Quote:
Guerrilla said:
Would have had a higher probability of success by pulling cotton filaments with your scalpel and burying those into multiple plates but fingers crossed.
Thanks for the tip! I’ll try that.
I do recommend dipping the swab in sterile water first though. It makes the cotton much easier to separate and hydrates spores too.
I use 8oz jars to PC my water but no pour containers would work too.
Since it was just sitting in the lab fridge, I diluted some leftover agar. It’s in the PC now. I figure dipping the swabs in sterile nutrient juice before shredding on the plate is superior to just water alone. Thanks again.
Have you tried my stubborn swab tek in my sig yet?
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AspectOfTheCreator
Mastering the Art of Success
Registered: 12/07/22
Posts: 1,447
Last seen: 3 hours, 58 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grogu]
#28165685 - 01/31/23 09:10 AM (1 year, 2 months ago) |
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I'm new to agar. I've done about 20 jars (1/2 pint) of all different kinds. BRF agar, LME agar, potato flakes/corn syrup agar, PDA. Inoculated via MS syringe, transfers, steaking with a loop, G2A, etc. Every single time the mycelium just seems absolutely intent on growing upwards rather than outwards. Am I just not worthy enough? Please educate me as to my blunder!
Another thing I find strange given my dodgy track record and being a noob, is that not a single plate has contammed on me. Not even when a drop of soapy water from the ceiling of my SAB fell into one of the plates while doing transfers (never misting the ceiling of my SAB again, only sides from now on). I've shined a high powered flashlight under the plate and it all just looks like solid white healthy mycelium every time.
Edited by AspectOfTheCreator (01/31/23 10:09 AM)
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ruawakeyet
Registered: 05/03/21
Posts: 1,862
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What's your agar recipe?
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