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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28165770 -

AspectOfTheCreator said:
I'm new to agar. I've done about 20 jars (1/2 pint) of all different kinds. BRF agar, LME agar, potato flakes/corn syrup agar, PDA. Inoculated via MS syringe, transfers, steaking with a loop, G2A, etc. Every single time the mycelium just seems absolutely intent on growing upwards rather than outwards. Am I just not worthy enough? Please educate me as to my blunder!

Another thing I find strange given my dodgy track record and being a noob, is that not a single plate has contammed on me. Not even when a drop of soapy water from the ceiling of my SAB fell into one of the plates while doing transfers (never misting the ceiling of my SAB again, only sides from now on). I've shined a high powered flashlight under the plate and it all just looks like solid white healthy mycelium every time.




Looks good. Just be patient. Itā€™ll grow out.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl] * 1
    #28166192 -

Does my growth seem slow to you? I've never felt like I've experience a spawn "run", but always merely a crawl. I am jealous when I read Paul Stamets saying a transfer will run across a plate in a week or two, I bet at this rate it would take 1 month to get a full plate. (slower if I do a few more transfers to clean the culture)

Transfer to plate on 1/21 (according to the image)

01/27/23


01/31/23


I have the plates in a styrofoam cooler tub with a glass jug of water w/ an aquarium heater inside. I've measured the heat inside the tub and even with the heater it barely gets much above 70.

I posted a few days ago about recipes, is it possible my recipe is bad or is this mostly just strain dependant?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: shitakee] * 5
    #28166200 -

:smile:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: labbar] * 1
    #28166217 -

Anyone every see a starfish like culture? Iā€™ve got a few plates I inoculated with a MSS that have grown out in a starfish like pattern, symmetrical triangle shaped gaps. 2 examples shown below along with a very rhizo culture and a MSS plate where a colony is growing up and over another one thatā€™s kinda cool.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ruawakeyet]
    #28166234 -

2 g agar, 1.6 g light malt extract, 100 mL dH2O.
3 g potato flakes, 2 g agar powder, 1 g corn syrup, 1/2 cup dH2O.
6:9:400 BRF:Agar:dH2O by mass.

Nothing exotic.


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Re: AGAR ENVY! (Anything and All things agar!) *DELETED* [Re: AspectOfTheCreator] * 1
    #28166283 -

Post deleted by Phaedra

Reason for deletion: X


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Phaedra]
    #28166331 -

Thanks for the heads up. You know what? I'm not even using telephone brand, I'm using Eden brand, not sure if that makes a difference. Didn't even consider that. Thank you for pointing out my potential oversight.


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Edited by AspectOfTheCreator (01/31/23 05:56 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Phaedra] * 1
    #28166339 -

Phaedra said:
AspectOfTheCreator said:
2 g agar, 1.6 g light malt extract, 100 mL dH2O.
3 g potato flakes, 2 g agar powder, 1 g corn syrup, 1/2 cup dH2O.
6:9:400 BRF:Agar:dH2O by mass.

Nothing exotic.


I bought my agar in bulk a long ass time ago, but if I use full strength to what most recipes show, my plates come out SUPER hard.  I've been, since noticing that, using 6.5g agar agar/400 mL H2O and much prefer it a little softer.  It still cuts and transfers as supposed to.  I just got some strong agar.  A lot of people who use "telephone" brand also find that it gels stronger than others.


:whathesaid:
I have Hoosier hill agar powder and had to adjust recipe to 6g per 500ml. Super strong stuff


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You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #28166347 -

I just started using Hoosier hill and I noticed it gets firmer than Telephone with same recipe as well.


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šŸ…ƒ šŸ„“ šŸ„° šŸ„¼    šŸ„² šŸ„» šŸ„ø šŸ„½ šŸ„¶ šŸ…† šŸ… šŸ„° šŸ„æ
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28166350 -

Eden brand is great but way too expensive.  Hoosier Hills is good and the price is right.


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šŸ…‚šŸ„øšŸ„¶šŸ„¼šŸ„°


Helpful Threads
stonesun
Mycoloradoā€™s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator] * 1
    #28166354 -

AspectOfTheCreator said:
2 g agar, 1.6 g light malt extract, 100 mL dH2O.
3 g potato flakes, 2 g agar powder, 1 g corn syrup, 1/2 cup dH2O.
6:9:400 BRF:Agar:dH2O by mass.

Nothing exotic.


Like others have said, try backing off the amount of agar agar powder. I prefer it as soft as possible and still able to make transfers. When I started doing that, I found I had a lot more visible contaminates, which would have been there all along.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28166370 -

Agreed. It's the only kind I can get OTC here from Whole Foods. Hence the price. Thanks for the advice I will adjust to make agar softer!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator] * 1
    #28167370 -

Great minds think alike, I too am using Hoosier Hill



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Be Kind to Everyone
Nobody can take away your Joy, you have to give it away willingly.
Instead, give them Mercy, give them understanding
But do not give away your Joy

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Skinflute]
    #28167378 -

Skinflute said:
Great minds think alike, I too am using Hoosier Hill




Still weird some companies call it agar agar powder instead of just agar.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl] * 1
    #28167446 -

Amazon just dropped a pound off yesterday. How does it do against telephone brand? Same amount, is it softer or harder?


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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #28167530 -

Much harder


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You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cozmyc] * 3
    #28167574 -

Azurescens T2 looking pretty good.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cozmyc] * 1
    #28167721 -

cozmyc said:
Much harder


Oh shit, I guess I'll take a gram or 1.5g off my totals


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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #28167772 -

Using Telephone brand, I'm down to 1.25% by weight for no-pour. With the amount of water I'm losing to evaporation it ends up being 1.34% by my calculation. (At the time I distribute the solution to each plate, before loading into the PCer. The water I'm losing is happening during the pre-cook/dissolving process.)

At 1.25%, it is still plenty firm enough to facilitate transfers, but I will be seeing exactly how low I can get it. I will try 1.2% as well as 1.15%. I tried 1.0% and it was definitely too soft.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ruawakeyet] * 3
    #28168103 -



EQ has been fun to work with. It's the fastest I've played with so far. This xfer was made 8 days ago.


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This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
Hobbitā€™s Secondhand Mycology Challenge
Round 2

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