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 Grogu
Registered: 07/02/22
Posts: 1,465
Last seen: 2 hours, 7 minutes
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 Re: AGAR ENVY! (Anything and All things agar!)  [Re: Tormato]  4
#28173993 - 02/05/23 08:50 PM (1 month, 20 days ago) |
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First "Wampa" clone on agar...
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Greg
always learning
Registered: 10/28/15
Posts: 1,510
Loc: an autoclave
Last seen: 1 day, 7 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grogu] 5
#28174114 - 02/05/23 09:32 PM (1 month, 20 days ago) |
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Some LAGM stuff that's grown out a bit.
 
Posted in general discussion but figured I'd post here too.
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L A G M 2 0 2 3
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Grogu
Registered: 07/02/22
Posts: 1,465
Last seen: 2 hours, 7 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Greg] 2
#28174160 - 02/05/23 09:52 PM (1 month, 20 days ago) |
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Quote:
Greg said:
Some LAGM stuff that's grown out a bit.
Posted in general discussion but figured I'd post here too.
These look really good, your photography is amazing...
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 Seeker604
Registered: 11/30/20
Posts: 279
Loc: United States 
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Greg] 2
#28174163 - 02/05/23 09:55 PM (1 month, 20 days ago) |
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Quote:
Greg said:
Some LAGM stuff that's grown out a bit.
Posted in general discussion but figured I'd post here too.
Beautiful!
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AspectOfTheCreator
Stranger
Registered: 12/07/22
Posts: 106
Last seen: 4 hours, 55 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Seeker604] 1
#28174666 - 02/06/23 09:51 AM (1 month, 19 days ago) |
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The fuzz I had transferred was apparantly trich. This happened overnight.
Still an interesting experiment. Going to try another tek to try to revive the dry sample
Edited by AspectOfTheCreator (02/06/23 09:52 AM)
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Eugene Gesuale
Jar-Sniffer
Registered: 04/12/20
Posts: 1,728
Loc: The Basement
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.
Edited by Eugene Gesuale (03/12/23 08:22 PM)
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Bobgas
Master of Mistakes
Registered: 09/01/22
Posts: 483
Last seen: 59 minutes, 28 seconds
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Eugene Gesuale] 1
#28175524 - 02/06/23 10:16 PM (1 month, 19 days ago) |
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I’m on T2 of HBOs ghost. The original germ plate looked quite good I thought and I’m now on T2. These look to me to need at least one more transfer.
 
They came from these donor plates.
I am wondering if I should take another transfer from the top left plate at 2 o’clock?
Ghost is a first for me. The myc is growing very differently than all other cubes I’ve got. Are my plates just still fucked and needing more transfers? I still don’t really know what I am looking at other than when myc looks completely uniform.
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Whyterye
Registered: 02/23/18
Posts: 1,038
Loc: Colorado
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas] 2
#28175631 - 02/07/23 12:54 AM (1 month, 18 days ago) |
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you have plenty of good rhizo spots to take transfers from. I would do like 5 transfers or so from that plate starting at 2 o clock and going down .
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fiddle_head
I'm not the dude, guy
Registered: 08/05/08
Posts: 857
Last seen: 2 days, 11 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] 3
#28175652 - 02/07/23 01:27 AM (1 month, 18 days ago) |
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Yeah that sector at 2 looks the most uniform n vigorous. I like clean uniform rhizo growth too before I send, though tomentose doesn't mean bad. Rhizo is just prettier.
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Scemilope
Mycoheretic
Registered: 11/16/22
Posts: 125
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Re: AGAR ENVY! (Anything and All things agar!) [Re: fiddle_head] 4
#28175950 - 02/07/23 08:38 AM (1 month, 18 days ago) |
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(72hrs)
This EQ is currently my fastest lil buddy.
I cut these with a 5mm punch.
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Parafilm taste, Cling-Wrap budget.
Psily-Shots
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Bobgas
Master of Mistakes
Registered: 09/01/22
Posts: 483
Last seen: 59 minutes, 28 seconds
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Scemilope]
#28177285 - 02/08/23 08:24 AM (1 month, 17 days ago) |
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I am right now PC’ing my agar media bottles without having loosened the lids. What a dumbass. I took them out of the micro, shook them and placed them straight into the PC and closed the lid. I just realized I never loosened the media lids again after the shake.
Is there anything I can do to save these bottles at this point?
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San Pedro Girl
𝓟𝓼𝔂𝓬𝓱𝓸𝓷𝓪𝓾𝓽
Registered: 07/17/12
Posts: 1,198
Loc: 🌎ℂ-𝟙𝟛𝟟
Last seen: 2 hours, 30 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas] 3
#28177300 - 02/08/23 08:36 AM (1 month, 17 days ago) |
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Quote:
Bobgas said:
I am right now PC’ing my agar media bottles without having loosened the lids. What a dumbass. I took them out of the micro, shook them and placed them straight into the PC and closed the lid. I just realized I never loosened the media lids again after the shake.
Is there anything I can do to save these bottles at this point?
Yeah, I would remove them from heat immediately and fix it.
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𝓛𝓪 𝓿𝓲𝓭𝓪 𝓮𝓼 𝓼𝓲𝓶𝓹𝓵𝓮, 𝓹𝓮𝓻𝓸 𝓲𝓷𝓼𝓲𝓼𝓽𝓲𝓶𝓸𝓼 𝓮𝓷 𝓱𝓪𝓬𝓮𝓻𝓵𝓪 𝓬𝓸𝓶𝓹𝓵𝓲𝓬𝓪𝓭𝓪.
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Bobgas
Master of Mistakes
Registered: 09/01/22
Posts: 483
Last seen: 59 minutes, 28 seconds
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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl] 1
#28177325 - 02/08/23 09:02 AM (1 month, 17 days ago) |
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Thanks SPG. I did just that. I am afraid that my agar is going to caramelize, but we’ll see what happens!
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Shrimps
Traveler
Registered: 10/13/22
Posts: 2,268
Loc: Under the sea
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas] 2
#28177326 - 02/08/23 09:04 AM (1 month, 17 days ago) |
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Better let your agar caramelize then breaking your bottles ;-)
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Bobgas
Master of Mistakes
Registered: 09/01/22
Posts: 483
Last seen: 59 minutes, 28 seconds
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shrimps]
#28177332 - 02/08/23 09:08 AM (1 month, 17 days ago) |
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Indeed!
Interestingly, when I pulled them out to loosen the lids, the agar inside the bottles was boiling - was the agar hot enough to be sterilized? Also, I use the corning media bottles and the lids are always a bit expanded due to the heat - when I tighten them down after a proper cycle, the threads are definitely expanded at least a bit.
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Steevil
Registered: 01/21/03
Posts: 103
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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl] 2
#28177341 - 02/08/23 09:11 AM (1 month, 17 days ago) |
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I always PC media bottles (and everything else) with the lids on tight, no problems so far. I believe RR did too.
Correct me if I'm wrong, but the water inside the bottles boils and pressurizes them, so they don't implode. And if pressure inside the bottles increases the boiling point also increases, so the water stops boiling and the bottles don't explode either.
Provided you don't vent the PC of course.
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San Pedro Girl
𝓟𝓼𝔂𝓬𝓱𝓸𝓷𝓪𝓾𝓽
Registered: 07/17/12
Posts: 1,198
Loc: 🌎ℂ-𝟙𝟛𝟟
Last seen: 2 hours, 30 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Steevil]
#28177523 - 02/08/23 11:25 AM (1 month, 17 days ago) |
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Quote:
Steevil said:
I always PC media bottles (and everything else) with the lids on tight, no problems so far. I believe RR did too.
Correct me if I'm wrong, but the water inside the bottles boils and pressurizes them, so they don't implode. And if pressure inside the bottles increases the boiling point also increases, so the water stops boiling and the bottles don't explode either.
Provided you don't vent the PC of course.
That’s very interesting. However, it messes up sterilization tables that assume all air has been expelled during a venting cycle.
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Please Vote if You Have Time!😊
𝓢𝓪𝓷 𝓟𝓮𝓭𝓻𝓸 G𝓲𝓻𝓵𝓼’ G𝓾𝓲𝓭𝓮𝓼
𝓛𝓪 𝓿𝓲𝓭𝓪 𝓮𝓼 𝓼𝓲𝓶𝓹𝓵𝓮, 𝓹𝓮𝓻𝓸 𝓲𝓷𝓼𝓲𝓼𝓽𝓲𝓶𝓸𝓼 𝓮𝓷 𝓱𝓪𝓬𝓮𝓻𝓵𝓪 𝓬𝓸𝓶𝓹𝓵𝓲𝓬𝓪𝓭𝓪.
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Mushymang
Stranger
Registered: 11/13/22
Posts: 99
Last seen: 11 days, 21 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl] 2
#28177975 - 02/08/23 04:43 PM (1 month, 17 days ago) |
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Amazoninan tissue clone plates here. This is t1, one transfer from the cloned tissue. First, would you all call that a t1? Second, I am trying to make sense of the two plates. One has rhizo growth that hopefully looks solid, it’s vigorous that’s for certain. Second plate (and there are a few like this) has half rhizo and what appears to be half tom growth. Is it common to see this variation from a tissue culture? Thank you.
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milkboy
shoeboxer
Registered: 05/01/21
Posts: 1,745
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mushymang]
#28177995 - 02/08/23 04:55 PM (1 month, 17 days ago) |
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Yes and yes
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fiddle_head
I'm not the dude, guy
Registered: 08/05/08
Posts: 857
Last seen: 2 days, 11 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] 1
#28178282 - 02/08/23 08:17 PM (1 month, 17 days ago) |
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Ape-R clone going along nicely.
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