Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Sporeworks
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Myyco.com APE Liquid Culture For Sale

Jump to first unread post Pages: < First | | 898 | 899 | 900 | 901 | 902 | 903 | 904 | 905 | 906 | 907 | 908 | 909 | 910 | 911 | 912 | 913 | 914 | 915 | 916 | 917 | 918 | | Last >
Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl] * 3
    #28039515 -

Day 6 after inoculation with Psilocybe Mexicana:




--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096]
    #28039550 -

Rusty2096 said:
HILLBILLY OUTLAW said:
I like to cut a swab into 4 petals and use it to knock 4 plates.


What do you cut it onto? The 1st plate (agar would look like WW2)? The inside of a plate cover?



bigfootscreepyuncl said:
more often than not I'll just jam the whole damn swab into the agar lol. Send a T1, if not germ plate wedge to grain


But that only works so often lol




Can someone tell me what is the white grow around the swab on the yeti plate?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroscopyUse]
    #28039552 -

For me it looks kind of like bacteria. But I would wait to hear what more experienced members have to say.


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Shrimps] * 1
    #28039591 -

Definitely bacteria

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #28039598 -

Is there any chance that the mycelium will outgrow it so he can make a transfer?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroscopyUse]
    #28039599 -

I don't see mycelium, only bacteria. I would say it's a lost cause... :frown:


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroscopyUse] * 1
    #28039602 -

Yes, give the plate time before discarding it.
He has a fair bit of real estate on the plate for growth so it can grow and he can transfer some clean mycelium.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #28039608 -

DERRAYLD said:
Yes, give the plate time before discarding it.
He has a fair bit of real estate on the plate for growth so it can grow and he can transfer some clean mycelium.


But it has been two weeks since he inoculated it. I am not so sure that there will be any mycelium growth. In my opinion the bacteria has already taken over the swab and keeps the mycelium from growin.

(But that is just a assumption, I could be wrong - so don't listen to much on what I say, I don't want to give false advice. It's how I would handle this case)


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Shrimps] * 1
    #28039641 -

I've had plates germinate after a few weeks that overtook bacteria giving me ample starting material to transfer.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #28039672 -

Also, it doesn't cost anything other than a tiny bit of space to just leave the plate and check it once a week.


--------------------

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD] * 1
    #28039679 -

DERRAYLD said:
Yes, give the plate time before discarding it.
He has a fair bit of real estate on the plate for growth so it can grow and he can transfer some clean mycelium.


It's bacteria but again that plate was posted to show.. bacteria.

I don't need to bother cleaning it because I already have another one that is clean.

Here - from the exact same qtip swab:
  VS 

Edit: @Shrips - if I really had to, I bet I could let it grow and manage to clean it. I've succeeded with worst. Here have a look :

(that culture was cleaned, grown, cloned and now is my favorite one atm)


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

Edited by Rusty2096 (11/08/22 05:26 AM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Shrimps] * 1
    #28039703 -

Shrimps said:
Day 6 after inoculation with Psilocybe Mexicana:





:creepylurker:


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 2
    #28039708 -

SirPsycho said:
Shrimps said:
Day 6 after inoculation with Psilocybe Mexicana:





:creepylurker:


Yeah, got them from a really awesome member here <3 :laugh: And I shared a bit of the print with an other member to spread the love :smile:

I am really happy how clean that print was, I can't see any contams from the inoculation.


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Edited by Shrimps (11/08/22 06:22 AM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Shrimps] * 1
    #28039874 -

Shrimps said:
I am really happy how clean that print was, I can't see any contams from the inoculation.


:whathesaid: Yeah, that dude is pretty awesome. We must have both started them at about the same time. Your growth is faster than mine, but I'm still trying to get my GWA on point so I'm not surprised. I'll be transferring to a new batch soon, so I hope it picks up some speed then.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 6
    #28040238 -

Phobos Squat iso project


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 5
    #28040350 -

APE swabs


--------------------
Check your vector

Gummies by milkboy
Milkboys Public Display of Affection
I'm a Semp


Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: milkboy] * 3
    #28040375 -

milkboy said:
APE swabs




Looking CLEAN MILKBOY!

MAK T4


--------------------
.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Atomsplit] * 1
    #28040395 -

Whats crackin Atomsplit! Long time no see :smoker:


--------------------
Check your vector

Gummies by milkboy
Milkboys Public Display of Affection
I'm a Semp


Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28040950 -

Yeti



and FP+



look different but nice...

:cookiemonster:


--------------------


Mushroom Cultivation Techniques...

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: milkboy]
    #28040957 -

GERMINATION Y'ALL!!!

Sorry for the excitement, my kids don't give a shit about microbiology yet, and I don't share this hobby with anyone else in my life, so you all have the privilege of dealing with my excitement here.

A couple lessons learned, I think.

First, I'm pretty sure my agar is too hard, that's why the first five plates I tried anything with did nothing. Did 10g agar, 10mg LME, 500ml and pre-boiled before PCing (learned on this thread that last step was a poor idea).

Second, pre-stretching the parafilm seems to be SOP. The parafilm has split on a handful of plates, so will be trying the pre-stretch next time.

Will also be trying 7g agar instead of 10g to 500ml water, see if softer agar works out better for germination.

Otherwise, here is a pic of an APE multispore plate I knocked up this past weekend. Of the three plates I hit with these spores, this is the only one that is showing signs of life. Also did three with psilocybe cyanescens, and three with pan cambo, and none of those have shown any signs of doing anything.



Just wanted to celebrate here a little and say thank you to all you fine people that have posted so much information, lessons learned, etc for decades. It's much appreciated! I'm working on a setup for better plate pics as well, so promise I'll quit dropping these bad ones here soon :smile:

Edited by thehiveatp (11/08/22 07:42 PM)

Extras: Filter Print Post Top
Jump to top Pages: < First | | 898 | 899 | 900 | 901 | 902 | 903 | 904 | 905 | 906 | 907 | 908 | 909 | 910 | 911 | 912 | 913 | 914 | 915 | 916 | 917 | 918 | | Last >

Shop: Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Myyco.com APE Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* CASO and Sabouraud-Glucose Agar: Any good ? JeRiKo1 2,256 5 04/19/04 04:24 AM
by JeRiKo1
* agar
( 1 2 all )		 arabmobster 5,001 21 04/11/03 04:10 PM
by arabmobster
* using agar? downforpot 1,113 2 10/21/01 12:19 PM
by oscill8
* Agar help the_pigdog 526 3 08/16/06 09:55 AM
by Roadkill
* Dam Agar the_pigdog 599 3 09/04/06 04:23 PM
by RogerRabbit
* buying agar - help sinisha 3,830 6 03/31/06 05:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 8,366 8 02/14/05 07:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,998 8 02/25/05 11:03 AM
by agar

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
769,343 topic views. 19 members, 506 guests and 141 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.05 seconds spending 0.028 seconds on 16 queries.