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Offlinearabmobster
#1

Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 15 years, 2 months
agar
    #1442013 - 04/09/03 05:45 AM (21 years, 3 months ago)

when yo scrap spores on the agar ow long will it take to see signs of growth

i used this method
I will3 pkgs Gelatine pkgs
1 1/2 cups of water
1 beef boullion cube

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OfflineTeKn0
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Re: agar [Re: arabmobster] * 1
    #1442055 - 04/09/03 06:18 AM (21 years, 3 months ago)

Approximately 3-4 days, Depending on strain/conditions.


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OfflinePaid
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Re: agar [Re: TeKn0]
    #1442061 - 04/09/03 06:23 AM (21 years, 3 months ago)

Yep about 3 to 5 days normally.But tell me,why are you using animal products to grow mushrooms on?


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Offlinearabmobster
#1

Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 15 years, 2 months
Re: agar [Re: Paid]
    #1442375 - 04/09/03 09:20 AM (21 years, 3 months ago)

why wont it grow
i got it off the shroomery
and its the simplest

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Offlinearabmobster
#1

Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 15 years, 2 months
Re: agar [Re: arabmobster]
    #1442468 - 04/09/03 09:53 AM (21 years, 3 months ago)


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: agar [Re: arabmobster]
    #1442549 - 04/09/03 10:23 AM (21 years, 3 months ago)

3 days minimum , and could take up to 10-14 days at the longest , this all depends on strain , enviro conditions etc ...
Beef bulion agar mix huh ?> Never heard of that , if you keep having bad luck , perhaps it is the agar mix . Sporeworks sells several types of agar , and one jar is plenty for the average grower , you might want to consider using a premade agar mix IMHO .
Good Luck
"May The Mushroom Goddess Bless Alll Your Endeavors !"


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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OfflinePaid
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Re: agar [Re: arabmobster]
    #1442561 - 04/09/03 10:28 AM (21 years, 3 months ago)

I never said it wouldnt grow i asked why you would wont to use meat products for a species that survives on celulose.Weather it works or not.Potatoes agar and water are pretty easy things to source?So i ask again why would you use meat products?Workerbal or not.


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: agar [Re: Paid]
    #1442579 - 04/09/03 10:34 AM (21 years, 3 months ago)

I agree , I also think contams can and will set in much faster using this mixture .


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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OfflineSpasey
newbie

Registered: 04/08/03
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Re: agar [Re: tripndicular]
    #1442614 - 04/09/03 10:43 AM (21 years, 3 months ago)

Plus who wants animal remnants in their shrooms, what if you give some to a vegetarian. It just doesnt seem pure

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Offlinearabmobster
#1

Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 15 years, 2 months
Re: agar [Re: Spasey]
    #1443197 - 04/09/03 01:12 PM (21 years, 3 months ago)

ya i guess i should use potatoes
thanks

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InvisiblePrisoner#1
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Re: agar [Re: Spasey]
    #1443767 - 04/09/03 04:27 PM (21 years, 3 months ago)

Quote:

Plus who wants animal remnants in their shrooms, what if you give some to a vegetarian. It just doesnt seem pure




I've been trying to feed a couple of them bacon.....why just go for meat when you can have pork....MMMMmmmmm....bacon.....

do you really think that there are any meat products in todays over proscessed foods....Mc Donalds is using 60% mushrooms in their chicken nuggets in many countries, and looking at the chemical makeup of most agars it contains peptone, where would we derive peptone from....did you know that mushies can grow in beef broth agar? the thing that you have to be aware of is the salt content...a quarter to a half of a boullion cube is a better amount...adding dextrose or potato flakes is even better....

you guys would be amazed at just how many substances spores will grow on....

Prisoner#1

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Anonymous

Re: agar [Re: Prisoner#1]
    #1443794 - 04/09/03 04:35 PM (21 years, 3 months ago)

what exactly is agar? and where do you buy it? I've heard it talked about many times but I have no clue what it is

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Offlinearabmobster
#1

Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 15 years, 2 months
Re: agar [Re: Prisoner#1]
    #1443796 - 04/09/03 04:36 PM (21 years, 3 months ago)

i would of never guessed it grows on meat

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OfflinePantherina
Alchemist

Registered: 03/02/03
Posts: 82
Loc: Nirvana
Last seen: 21 years, 21 days
Re: agar [Re: arabmobster]
    #1443830 - 04/09/03 04:48 PM (21 years, 3 months ago)

Stupaod, read the faq. (agar=seaweed extract, where do you buy it=check out the sponsors page or the search option)




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The man who lost the plot, so had to improvise

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Offlineshirley knott
not my real name
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Re: agar [Re: Pantherina]
    #1443856 - 04/09/03 04:57 PM (21 years, 3 months ago)

or in chinese / asian supermarkets


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buh

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OfflineUnknown
Confused,dizzy,wheream I again?
Registered: 01/06/02
Posts: 380
Loc: MA
Last seen: 20 years, 3 months
Re: agar [Re: arabmobster]
    #1444034 - 04/09/03 06:06 PM (21 years, 3 months ago)

Prisoner #1 gave me this tek and I posted it on here. If you use powdered potato flakes it will work much better for use with cubes,some other species prefer the boullion though.


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The above is just like,my opinion man

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InvisiblePrisoner#1
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Re: agar [Re: Anonymous]
    #1444568 - 04/09/03 08:39 PM (21 years, 3 months ago)

Food grade agars are produced with gracilaria or gelidiella seaweed's and the gel strength of food grade agar is lower than bacteriological grade agar... gelling temperature of food grade agar is also higher than that of bacteriological grade.

bacteriological grade agar is only extracted from gelidium sesquipedalis seaweed's. Agar is colloidal substance...it is insoluble in cold water... but disolve in boiling water. After this is done to needs to be autoclaved in order to ensure it's sterility. Agar is of course what you grow multitudes of different bacteria, molds, fungi and other pathogens on... so in short...Agar is a vegetable based gelatin.....I think all of this is in the FAQ


Prisoner#1

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InvisiblePrisoner#1
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 193,665
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Re: agar [Re: Prisoner#1]
    #1444589 - 04/09/03 08:46 PM (21 years, 3 months ago)

almost forgot....you can learn more about agar at www.agar.net

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Invisiblewoodrow
journeyman
Registered: 03/17/03
Posts: 142
Re: agar [Re: arabmobster]
    #1445567 - 04/10/03 01:53 AM (21 years, 3 months ago)

i used this method
I will3 pkgs Gelatine pkgs
1 1/2 cups of water
1 beef boullion cube
-----------------------------

Gelatine is not the same as agar and it is a poor substitute.

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Offlinearabmobster
#1

Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 15 years, 2 months
Re: agar [Re: woodrow]
    #1445773 - 04/10/03 05:18 AM (21 years, 3 months ago)

did it wotk with you woodrow
did any of them get infected with bacteria

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Invisiblewoodrow
journeyman
Registered: 03/17/03
Posts: 142
Re: agar [Re: arabmobster]
    #1448810 - 04/11/03 03:28 AM (21 years, 3 months ago)

Any agar can become infected with bacteria. If the agar becomes contaminated with bacteria, it often works to flood the agar with peroxide straight from the bottle and swish it around for a few seconds and drain. Two quick rinses with peroxide will wash most bacteria away. This does not work if the contam is a mold.
I don't think the recipe would work well for shrooms because of the salt in beef bullion. Shrooms will grow on beef broth but bacteria do even better. Gelatine is not good because it is liquid at room temp unless you make it extra thick and many things that grow on gelatine can digest it and turn it to liquid in a hurry. Agar is good because it stays solid and almost nothing can digest it.
Anno's dog food agar is good for shrooms and malt extract agar is also good.
You can take just about anything (a little dog food, corn meal, BRF, V-8 juice, green beans, lima beans, molasses) cook them up, pour off the liquid, add agar and pressure cook the mixture to make an agar that is just as good as any agar you can buy. Agars with a lot of sugar discourage the growth of bacteria. You can add 4% Karo corn syrup to an agar recipe if it lacks sugar to make it more selective.


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Offlinearabmobster
#1

Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 15 years, 2 months
Re: agar [Re: woodrow]
    #1450361 - 04/11/03 04:10 PM (21 years, 3 months ago)

thank you ver much

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