shroomsurf said:

So my LC is quite colonized and I put some drops on agar for both LC media bottles...

What am I looking for in terms of contamination/bacterial exactly?

Also I love LC so far, only in 7 days it's ready and if I can get it to be clean I might actually do G2G if you guys think that's a good idea?

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Shrimps said:

shroomsurf said:

So my LC is quite colonized and I put some drops on agar for both LC media bottles...

What am I looking for in terms of contamination/bacterial exactly?

Also I love LC so far, only in 7 days it's ready and if I can get it to be clean I might actually do G2G if you guys think that's a good idea?

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Well for a start, there shouldn't be any growth exept from the mycelium whatsoever.

If you only have mycelium growing, and not showing any signes of contaminations, that's what your aiming for

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The LC media bottles passed the turbidity test and it's fairly clear although I used tap water and I heard that distilled is clearer...



I guess I'll post again in a couple days with my agar cups to let you guys take a look...

Oh yea, and should I keep it in fridge or just in cubbard, my home is about 60-70F all the time with about 50'ish humidity...

Thanks...

Rusty2096 said:

I doubted a friend.

A very cool member here sent me 2 Yeti qtips swabs.
I used 1 to streak 4 plates and they all grew a lot of nothing...so I kinda thought he had swabbed nothing or not sporulating fruits

Except this one (13 days later which seems like forever for fresh spores)


1 single very lonely colonie, looking extremely clean!!!

Thank you again Screwup!

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I busted out the microscope and carefully found the two slowest spores I could find to send to you

Rusty2096 said:

I doubted a friend.

A very cool member here sent me 2 Yeti qtips swabs.
I used 1 to streak 4 plates and they all grew a lot of nothing...so I kinda thought he had swabbed nothing or not sporulating fruits

Except this one (13 days later which seems like forever for fresh spores)


1 single very lonely colonie, looking extremely clean!!!

Thank you again Screwup!

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HILLBILLY OUTLAW said:

I like to cut a swab into 4 petals and use it to knock 4 plates.

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bigfootscreepyuncl said:

more often than not I'll just jam the whole damn swab into the agar lol. Send a T1, if not germ plate wedge to grain

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But that only works so often lol

Rusty2096 said:

But that only works so often lol

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IDK man. Sounds to me like dirty spores or bad technique. I've got about a dozen pics somewhere of swab sticks, sticking straight out of clean myc on a germ plate

I've got a set of PE swabs from a certain member that I'm so confident in I think I'm just going to just snip the tip into a grain jar



Did you ever get that AA+ cleaned up?

Quote:

bigfootscreepyuncl said: I think I'm just going to just snip the tip into a grain jar

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SirPsycho said:

Quote:

bigfootscreepyuncl said: I think I'm just going to just snip the tip into a grain jar

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Somewhere a TC just had an aneurysm

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a TC was just born. this is some true boss shit

Quote:

bigfootscreepyuncl said: I think I'm just going to just snip the tip into a grain jar

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I remember Cronicr doing that a long time ago. Next time I pull spores from a plate pin I'll give it a go lol

Rusty2096 said:

???

That AA was super clean all along. The discoloration you see is only the myc eating the colored grain water

(it does that on literally all my plates. The 2 side x side pics where meant to show clean vs not clean)

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