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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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the_pigdog
Myco Master

Registered: 05/06/06
Posts: 71
Last seen: 14 years, 8 months

Dam Agar

#6027472 - 09/04/06 08:51 AM (17 years, 7 months ago)

All of my 7 petri dish's have got contaiminated, i was wondering if it was the receipe or a airborn contam or the contensation or the steralization,

The Recipe i was using was:
per 250ml water
3.75g Agar
1.25 Peptone
0.75g Vegemite (Yeast extract)

Filled with boiling water, stried and then microwaved for 3mins HIGH and then on 20minutes on low/defrost and then poured before it got to cold, taped then refridgerated for a week then inculated.

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Prisoner#1
Even Dumber ThanAdvertized!

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck

Re: Dam Agar

[Re: the_pigdog]
#6027554 - 09/04/06 09:21 AM (17 years, 7 months ago)

Quote:

the_pigdog said:
microwaved

this evil device would be my guess

--------------------
California Has Legalized Child Prostitution

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ar393
old timer

Registered: 01/26/00
Posts: 702
Loc: VT

Re: Dam Agar

[Re: the_pigdog]
#6027605 - 09/04/06 09:39 AM (17 years, 7 months ago)

I don't know about the microwave technique for sterilization, but 15 minutes at 15 psi, pour and in about 2-3 hours they are ready to go.

taping them up and popping them in your fridge probably didnt help, also the more you handle it the more contams are likely.

I usually just pour them and leave them on my counter for a bit and inncoulate within 24 hours. in fact there are a few sitting and waiting for me now.

-ar393

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RogerRabbit
Bans for Pleasure

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month

Re: Dam Agar

[Re: ar393]
#6028767 - 09/04/06 04:23 PM (17 years, 7 months ago)

You can't sterilize agar with a microwave. You'll need a pressure cooker.

You should also use a container that has a filtered lid, and sterilize the agar with the lid in place. The filtered lid protects the agar as it cools.
RR

--------------------
Download Let's Grow Mushrooms

semper in excretia sumus solim profundum variat

"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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