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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #28001726 -

Smellyhobbit said:
Bajazly said:
Smellyhobbit said:
Some cultures I have going I wanted to share. Love how the maza played out.




Sexy AF, nice.

What is the point with the boob plate? Why two pieces in one plate?


Early on I’ll do two small transfers on the same plate instead of burning a second plate looking for clean myc.

Going forward each transfer gets a single plate. Once it gets to testing.



Well that makes infinite sense. Nice looking plates there.

Can't wait till I start getting growth like that.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bajazly] * 3
    #28002411 -


Playa x Fiji T2.


Rusty Whyte T3 (T2 was a water-agar clean up job, worked great.)


Mystery swab T2: maybe Melmak, maybe Natalensis.

All three MS cultures, probably go to grain next weekend for a round of tests.
:airguitar:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28002421 -

pretty cultures!
how did you get rid of condensation from the lid of those cups?
I'd bet my left nut that's not natalensis myc :P


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“All this buttoning and unbuttoning”

Edited by 999999999 (10/16/22 09:27 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 999999999] * 2
    #28002423 -

Gleyck said:
pretty cultures!
how did you get rid of condensation from the lid of those cups?
I'd bet my left nut that's not natalensis myc :P


I’ve got another Nat culture from a known source and it has produced some crazy variations in myc morphology. Most have not been this gently tomentose, some have been downright weird lookin. So we’ll see. I’d be hyped as fuck on some Melmak either way.

…all the typical methods. To some degree it’s inevitable in these cups (you can see the tiny micro-drops on the walls in the pics above). But I don’t let it bug me too much. The most effective prevention strategy I’ve found is to reduce the temperature differential as much as possible between the air inside the cup when pouring/sealing the lid, and the temp of the air outside the cup while the media sets. So I never pour until my media is at or below 125F (I like softer recipes so it doesn’t gel as soon), I prefer to pour in a warm setting when humidity is low, and allow sealed cups to cool slowly by stacking warm, and storing where they can stay warm for a few days until ready to use. Some will always have condensation on the lid. I just set those aside and use the clear ones first, a week passes and when I’m ready to do more work, the others have mostly cleared up.


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Edited by Egon_Spengler (10/16/22 10:06 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Egon_Spengler]
    #28002753 -

Agar advice wanted please:

This is T0 of JMF. Spore Print to PDA agar. This one grew out pretty fast.



This is also a T0 taken at the same time with (Corn syrup/Potato flakes) agar. This one looks better to me, but also grew out slower.


This is a T1 (mislabeled as T2) that I took from the first T0 above. The agar is MEA.


I read somewhere a while ago it's good to switch up your agar nutrients because if you don't, myc can become lazy as to what it eats. But, as you can see in the T1, it's super whispy, while the parent T0 is not.

Should I bother taking another transfer from the T1, or just start from base again and do a new set of T1's from the T0's. If so, which T0 would you choose?

Thanks in advance!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28003087 -

These have been transferred 3 times. Two different species. Both sclerotia. I am new to this so, are these samples considered isolates? Should I be seeing more brown spots since this is sclerotia? And if so, why am I not seeing some? Thanks guys!



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Fear is the mind killer

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mystic Merlin] * 2
    #28003151 -

They're isolates in one way but not another.

Looks like you've isolated mycelium you want away from contaminates you don't want.

As far as being genetic isolates though, I think they are a ways off yet.


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #28003173 -

LotKid said:
They're isolates in one way but not another.

Looks like you've isolated mycelium you want away from contaminates you don't want.

As far as being genetic isolates though, I think they are a ways off yet.


Ok thanks! I will keep transferring and see where this road goes :smile:


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Fear is the mind killer

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mystic Merlin] * 2
    #28003192 -

Chasing down a single set of isolated genes the way youre probably thinking isnt really super beneficial.

Having healthy clean cultures is the primary goal. Looks like youre on the right track with that. I've usually noticed stones forming on plates when theyre a lil more mature. If youre looking to transfer stones then just give em time and grab one when it forms.


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #28003276 -

LotKid said:
Chasing down a single set of isolated genes the way youre probably thinking isnt really super beneficial.

Having healthy clean cultures is the primary goal. Looks like youre on the right track with that. I've usually noticed stones forming on plates when theyre a lil more mature. If youre looking to transfer stones then just give em time and grab one when it forms.


Ah, thank you for that! Makes a ton of sense, I just needed to hear it.


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Fear is the mind killer

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28003374 -

Where is the best online source for bulk (over 100 but less than 500) agar plates?  Looking for cost and consistently sterile plates.

What's a good "soft" LME agar recipe?  Should I do 8g agar, 8 grams LME, and 400ml H20? 

I had issues with contams in my plates and chunky agar and condensation and not super clear agar last time, so I'm stepping up my game this time.

What type of filter and where is a good online source for flow hood filters?  Is there a good TEK to describe how to make a hood?  I don't need step by step so much, but more of the concepts behind a good hood design and why.


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Edited by Rabbit84 (10/17/22 04:18 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rabbit84]
    #28003440 -

Rabbit84 said:
Where is the best online source for bulk (over 100 but less than 500) agar plates?  Looking for cost and consistently sterile plates.

What's a good "soft" LME agar recipe?  Should I do 8g agar, 8 grams LME, and 400ml H20? 

I had issues with contams in my plates and chunky agar and condensation and not super clear agar last time, so I'm stepping up my game this time.

What type of filter and where is a good online source for flow hood filters?  Is there a good TEK to describe how to make a hood?  I don't need step by step so much, but more of the concepts behind a good hood design and why.


i think p9 has a good thread on that


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Confused? Well now you can!
HHG - cheapest way to start - how i roll

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bakedbeings]
    #28003521 -

Does this look like it could be mycelium and mold?  A lot of my pasty plates look like this.  It seems pretty uniform but the myc looks really tomentose and it makes think it could be mold.  Sorry for the shitty pics, unfortunately this is about as good as it gets for pasty plates. All of these pics are of the same plate. 








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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheGreatDellion]
    #28003558 -

IDK, looks pretty good to me but I don't know a lot. I've got some that look the same and I think they are doing pretty well but again, I don't have a lot of experience with this. I'll be interested in the answers you get.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheGreatDellion]
    #28003589 -

Looks fine


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Check your vector

Gummies by milkboy
Milkboys Public Display of Affection
I'm a Semp


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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheGreatDellion]
    #28003595 -

I see clean myc


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Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 3
    #28003634 -



--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28003644 -

Charcoal?


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28003657 -

nektar61 said:



Very cool looking, nice.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid] * 1
    #28003766 -

LotKid said:
Chasing down a single set of isolated genes the way youre probably thinking isnt really super beneficial.

Having healthy clean cultures is the primary goal.


I agree with this. If you're looking for better genetic isolation cloning is far superior to doing multiple agar transfers originating from a spore plate.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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