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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Screwup] * 1
    #27949338 -

Screwup said:
Shiiieet I remember what happened the last time some Germans got pissed. Gonna see myself to the exit.


You better come to our party, or we'll take you by fooo--ooorce! :P

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Silandril]
    #27949372 -

If a plate of agar stalls will a transfer help or is it just donesies?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shwingdings9844] * 1
    #27949387 -

Could be senescence (idk how old that myc is), could be it doesn't like the nutes, could be tam. IMO best way is to transfer a small piece from leading edge to a diff nutes plate to find out.


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27949388 -

:whathesaid:


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shwingdings9844]
    #27949389 -

How do I end this friend?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla] * 2
    #27949400 -

Guerrilla said:
No he's just German


:curbyourenthusiasm:


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27949679 -

I'm game to try agar after having injected spores to BRF with mixed results.

I have a print (B+) and a couple of syringes of PE to work with.

I believe I have almost all that I need in reading through lots of posts, and plan to start with an MEA based recipe.

For petri dishes (rather than no pour) glass seems to be ridiculously expensive and I am trying hard not to waste money getting started in this hobby!

Do most folks go with plastic dishes?  I see there are "vented" lids and assume this is not what I want.

Thanks for the help!


--------------------
๐Ÿ…ƒ๐Ÿ„ด๐Ÿ„ฐ๐Ÿ„ผ  ๐Ÿ„ฒ๐Ÿ„ป๐Ÿ„ธ๐Ÿ„ฝ๐Ÿ„ถ๐Ÿ…†๐Ÿ…๐Ÿ„ฐ๐Ÿ„ฟ

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas]
    #27949687 -

Bobgas said:
I'm game to try agar after having injected spores to BRF with mixed results.

I have a print (B+) and a couple of syringes of PE to work with.

I believe I have almost all that I need in reading through lots of posts, and plan to start with an MEA based recipe.

For petri dishes (rather than no pour) glass seems to be ridiculously expensive and I am trying hard not to waste money getting started in this hobby!

Do most folks go with plastic dishes?  I see there are "vented" lids and assume this is not what I want.

Thanks for the help!


Disposable Petri dishes are about 500 units for $100 USD. Pretty good. You can get like 5 Pyrex plates for that cost. I personally use plastic. If I had access to glass it would unquestionably be what I used.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #27949715 -

Today is a big win for me (a little early to call it that but I'm very hopeful).

I did cultivate a bit many many years ago, mostly as a bystander, learning while trying to help a friend cult.A few weeks ago, I decided I wanted to make this a hobby for myself and started learning as much as I could. I did initiate myself to agar, with the help of this community and countless hours of reading.

My 1st agar project was to clean a bacterial GT LC (I know, not the best place to start). LC inoc'd plates were straight up gross. I did many T2s. Few of them were looking much better.

Today I did 4 T3s and I'm confident they will come out clean (/pray).


Donor plate:


T3s:


While I was at it, today I also knocked-up a few plates with BM LC, hoping this one won't be as bad as the GT LC I had (only 1 pic because not much to see here).



PS: Some of my plates were wrapped with 2'' micropore tape because I was out of 1'' last time I did agar. IT WAS HORRIBLE. Hard to remove, all the plates were sticky. Today I used parafilm and I will never go back to micropore tape.


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

Edited by Rusty2096 (09/14/22 04:58 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #27949927 -

Babyโ€™s first plin. Cloned APE.



--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #27949950 -

Do I have to have a pressure cooker to make agar? Or could I just boil the jar in a pot?


--------------------
My Nichromeโ€™s Wirelock Challenge Growalong

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AGAR ENVY! (Anything and All things agar!) [Re: jakemp] * 2
    #27950180 -

jakemp said:
Do I have to have a pressure cooker to make agar? Or could I just boil the jar in a pot?


You play with fire when you don't use a PC for agar. Just do yourself the big solid and use it. Your future self will thank you. :rockon:


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #27950199 -

Smellyhobbit said:
Babyโ€™s first plin. Cloned APE.




:bender:

Congrats!


--------------------

Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #27950203 -

I friggin love your avatar @Tormato


--------------------
You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27950210 -

Tormato said:
jakemp said:
Do I have to have a pressure cooker to make agar? Or could I just boil the jar in a pot?


You play with fire when you don't use a PC for agar. Just do yourself the big solid and use it. Your future self will thank you. :rockon:


I don't think he owns one.

Tormato is right, PC for sure is the best way to do it. But if you want to try agar in the meantime (before you get your hands on a PC), you can sterilize your media bottle and it's content in a pot or even in the microwave. Not optimal but it works,


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096]
    #27950309 -

Yeah I dont have one.. I was thinking of borrowing a instant pot from a friend.
Do those also get to 15psi? Or should I just get a pressure cooker?


--------------------
My Nichromeโ€™s Wirelock Challenge Growalong

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096] * 1
    #27950316 -

Rusty2096 said:
Tormato said:
jakemp said:
Do I have to have a pressure cooker to make agar? Or could I just boil the jar in a pot?


You play with fire when you don't use a PC for agar. Just do yourself the big solid and use it. Your future self will thank you. :rockon:


I don't think he owns one.

Tormato is right, PC for sure is the best way to do it. But if you want to try agar in the meantime (before you get your hands on a PC), you can sterilize your media bottle and it's content in a pot or even in the microwave. Not optimal but it works,


Implying get one obviously if not owned it's in my signature :super:

I stand by playing with fire, but people get lucky. :goodluck:

jakemp said:
Yeah I dont have one.. I was thinking of borrowing a instant pot from a friend.
Do those also get to 15psi? Or should I just get a pressure cooker?


Instapots work. Too be fair you might even get lucky with the boiling water method, but in best practice it's crucial to get to sterilization temps and sustain it for the minimum duration.

*Side note: this shit sucks editing and quoting using a phone.... FML I need a desktop or something :facepalm:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 3
    #27950336 -

Just got off the phone with someone on craigslist, getting a 23 quart presto for $80 bucks. Used once :thumbup:


--------------------
My Nichromeโ€™s Wirelock Challenge Growalong

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Re: AGAR ENVY! (Anything and All things agar!) [Re: jakemp]
    #27950344 -

jakemp said:
Just got off the phone with someone on craigslist, getting a 23 quart presto for $80 bucks. Used once :thumbup:


Score dude! Thatโ€™s awesome.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: jakemp]
    #27950347 -

jakemp said:
Just got off the phone with someone on craigslist, getting a 23 quart presto for $80 bucks. Used once :thumbup:


Niiiiice! You got a good deal if it's in good shape!! See...it's just a matter of timing :cheers:


And ninja edits :wink:

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Shop: Myyco.com APE Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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